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Association between p21 Ser31Arg polymorphism and the development of cervical lesion in women infected with high risk HPV

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Tumor Biology

Abstract

Infection by high-risk human papillomavirus (HR-HPV) and single nucleotide polymorphism (SNP) in genes involved in cell cycle control, as p21 and p27, are important factors in the development of different types of human cancers. This study aims at investigating whether both the p21 Ser31Arg and p27 V109G polymorphisms are associated with susceptibility to the development of cervical lesions in women HR-HPV positive. We analyzed 132 women HPV positive and with cervical lesions or CC and 154 healthy control (HPV negative and without cervical lesions). p21 Ser31Arg and p27 V109G polymorphisms were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and sequencing. The p21 31Arg allele was associated with susceptibility for the development of cervical lesions (P* = 0.0009), while p27 V109G polymorphism showed no significant differences for this association (P* = 0.89). However, the combined effect of the polymorphisms showed that the presence of the CC genotype (SNP p21 Ser31Arg) conferred protection for the development of cervical lesions (OR = 0.39). p21 Ser31Arg and p27 V109G polymorphisms were not associated with the grade of cervical lesions (CINI, CINII, and CINIII) or CC (P* > 0.05). The HR-HPV more frequent in this study were of 16 (57.6 %) and 18 (37.1 %) types; however, no association was observed when both polymorphisms and risk factors analyzed were compared (P* > 0.05). Our findings suggest a possible association between p21 Ser31tabArg polymorphism and susceptibility to the development of cervical lesions in women from Pernambuco. Brazil.

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Authors and Affiliations

  1. Post-Graduate Program in Cellular and Molecular Biology Applied, University of Pernambuco (UPE), Recife, PE, Brazil

    Géssica Lima & Paulo Souza

  2. Post-Graduate Program in Tropical Animal Science, Rural Federal University of Pernambuco (UFRPE), Recife, PE, Brazil

    Erinaldo Santos, Maria Maia & Paulo Souza

  3. Post-Graduate Program in Genetic, Federal University of Pernambuco (UFPE), Recife, PE, Brazil

    Hildson Angelo & Sergio Crovella

  4. Federal Institute of Pernambuco – Campus Garanhuns (IFPE/Garanhuns), Garanhuns, PE, Brazil

    Hildson Angelo

  5. Post-Graduate Program in Biological Science, Federal University of Pernambuco (UFPE), Recife, PE, Brazil

    Micheline Oliveira

  6. Departament of Lower Genital Tract Pathology, Women’s Healthcare Center, Instituto de Medicina Integral Prof. Fernando Figueira (IMIP), Recife, PE, Brazil

    Sandra Heráclio

  7. Department of Biology, Rural Federal University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, PE, Brazil

    Fernanda Leite, Maria Maia & Paulo Souza

  8. Departament of Physics, Federal University of Pernambuco (UFPE), Recife, PE, Brazil

    Celso de Melo

  9. Departament of Genetics, Federal University of Pernambuco (UFPE), Recife, PE, Brazil

    Sergio Crovella

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  1. Géssica Lima
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Correspondence to Paulo Souza.

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The work was performed at the Rural Federal University of Pernambuco (UFRPE), Recife, PE, Brazil.

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Lima, G., Santos, E., Angelo, H. et al. Association between p21 Ser31Arg polymorphism and the development of cervical lesion in women infected with high risk HPV. Tumor Biol. 37, 10935–10941 (2016). https://doi.org/10.1007/s13277-016-4979-0

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