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miR-448 suppressed gastric cancer proliferation and invasion by regulating ADAM10

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Tumor Biology

Abstract

MicroRNAs (miRNAs) are a class of short, noncoding RNAs that act a crucial role in tumor development. Previous studies showed that miR-448 expression was deregulated in many tumors. However, the role of miR-448 in gastric cancer (GC) remains unknown. In our study, we demonstrated that miR-448 expression was downregulated in GC tissues compared with the corresponding nontumor tissues. We also showed that miR-448 expression was downregulated in GC cell lines. Ectopic expression of miR-448 suppressed GC cell proliferation, colony formation, and invasion. Moreover, we identified A Disintegrin And Metalloproteinases 10 (ADAM10) as a direct target gene of miR-448 in GC cell. ADAM10 expression was upregulated in GC tissues and cells. Furthermore, the expression level of miR-448 was negatively correlated with the expression level of ADAM10 in GC tissues. Moreover, ADAM10 overexpression rescued the effect of miR-448-mediated GC cell proliferation, colony formation, and invasion. These results demonstrated that miR-448 might play as a tumor suppressor miRNA partly through targeting ADAM10 expression.

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Authors and Affiliations

  1. Department of Gastrointenstinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China

    Xuesong Wu, Haoran Tang, Jie Shu & Feng Sun

  2. Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China

    Guobin Liu

  3. Department of Gastroenterology, The Affiliated YanAn Hospital of Kunming Medical University, Kunming, 650051, Yunnan, China

    Hui Wang

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  1. Xuesong Wu
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  2. Haoran Tang
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  4. Hui Wang
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  5. Jie Shu
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  6. Feng Sun
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Correspondence to Feng Sun.

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Xuesong Wu and Haoran Tang are co-first authors.

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Wu, X., Tang, H., Liu, G. et al. miR-448 suppressed gastric cancer proliferation and invasion by regulating ADAM10. Tumor Biol. 37, 10545–10551 (2016). https://doi.org/10.1007/s13277-016-4942-0

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  • DOI: https://doi.org/10.1007/s13277-016-4942-0

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