Skip to main content
SpringerLink
Log in

Proteome alteration in human colon cancer cells by the treatment of HangAmDan-B

  • Original Research
  • Published:
BioChip Journal Aims and scope Submit manuscript

Abstract

Korean traditional medicine, HangAmDan (HAD) was developed in 1996 for anti-tumor purpose, and has shown positive results in clinical case studies. Recently, HAD has been recently modified as HAD-B to increase therapeutic effects. Our present study focused on the effect of HAD-B on proteome alteration in human colon cancer cells with highly aggressive invasiveness. Before the proteome assessment, invasiveness of 4 human colon cancer cell lines, SNU-407, SNU-C4, LoVo, and DLD-1 have been determined. Among cell lines tested, DLD-1 showed higher invasiveness, and was selected for investigating proteome affected by HAD-B. HAD-B reduced invasiveness of DLD-1, but it did not lead to either synergistic effect with 5-fluorouracil or apoptosis. Phosphorylation of eukaryotic initiation factor 4E (eIF-4E) binding protein 1 (4EBP1) and mammalian target of rapamycin (mTOR) in DLD-1 was increased by HAD-B treatment whereas level of AKT phosphorylation was reduced. Furthermore, expression of eIF-5A-2 and the balance between tissue inhibitor of metalloproteinases-1 (TIMP-1) and matrix metalloproteinase 9 (MMP9) were deranged in DLD-1 after treatment of HAD-B. In conclusion, overall results from our proteome assessment may provide useful information on how HAD-B suppresses proliferation and invasion of human colon cancer cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. O’Connell, J.B., Maggard, M.A. & Ko, C.Y. Colon cancer survival rates with the new American Joint Committee on Cancer sixth edition staging. J. Natl. Cancer Inst. 96, 1420–1425 (2004).

    Article  Google Scholar 

  2. Hecht, J.R. Current and emerging therapies for metastatic colorectal cancer: applying research findings to clinical practice. Am. J. Health Syst. Pharm. 65, S15–21 (2008).

    Article  CAS  Google Scholar 

  3. Christofori, G. New signals from the invasive front. Nature 441, 444–450 (2006).

    Article  CAS  Google Scholar 

  4. Choi, B.L. et al. Study on the toxicity of HangAm-Dan (HAD) in mice. J. Korean Oriental Oncol. 8, 23–35 (2002).

    Google Scholar 

  5. Lee, N.H. et al. Changes of serum VEGF, bFGF levels and platelet counts in 100 cancer patients treated with Hang-Am-Dan. Korean J. Orient. Int. Med. 26, 753–760 (2005).

    Google Scholar 

  6. Lee, D.E. et al. Antitumor effect of Hang-Am-Dan (HAD) and its ingredients on Calu6 and MCF-7 human cancer cell lines. J. Korean Oriental Med. 30, 50–60 (2009).

    Google Scholar 

  7. Kim, J.L. et al. Antitumor effect of Hang-Am-Dan non-boiled water extracts on NCI-H460 tumor regression model. J. Korean Oriental Med. 31, 34–46 (2010).

    Google Scholar 

  8. Choi, J.H. et al. Regression of small cell lung carcinoma byherbal medicine: a case with an 8-year follow-up. J. Korean Oriental Oncol. 10, 87–91 (2005).

    Google Scholar 

  9. Yoo, H.S. et al. A review of the best case series methodology: BCS program results of East-West Cancer Center. Integr. Cancer Ther. 7, 182–188 (2008).

    Article  Google Scholar 

  10. Park, B.K. et al. Retrospective cohort analysis for lung cancerpatients treated with Wheel Balance Therapy (WBT). Korean J. Orient. Int. Med. 29, 45–56 (2008).

    Google Scholar 

  11. Yoo, H.S. et al. The effects of HangAmDan (HAD) on anti-metastasis and preventing relapses, administered to 69 cancer patients. Korean J. Orient. Int. Med. 23, 165–173 (2002).

    Google Scholar 

  12. Bang, J.I. et al. Anti-Angiogenic effect of the water extract of HangAmDan, a Korean medicinal, using antibody microarray chip. BioChip J. 4, 350–355 (2010).

    Article  CAS  Google Scholar 

  13. Choi, Y.J. Inhibition of Cell Motility and Invasion by HAD-B in human non-small cell lung cancer NCIH460 Cells. Ph.D Thesis, Daejeon University, Daejeon, Korea.

  14. Itoh, N. et al. Phosphorylation of Akt/PKB is required for suppression of cancer cell apoptosis and tumor progression in human colorectal carcinoma. Cancer 94, 3127–3134 (2002).

    Article  CAS  Google Scholar 

  15. Dumstorf, C.A. et al. Modulation of 4E-BP1 function as a critical determinant of enzastaurin-induced apoptosis. Mol. Cancer Ther. 9, 3158–3163 (2010).

    Article  CAS  Google Scholar 

  16. Zhou, H. & Huang, S. Role of mTOR Signaling in Tumor Cell Motility, Invasion and Metastasis. Curr. Protein Pept. Sci. 12, 30–42 (2011).

    Google Scholar 

  17. Rojo, F. et al. 4E-binding protein 1, a cell signaling hallmark in breast cancer that correlates with pathologic grade and prognosis. Clin. Cancer Res. 13, 81–89 (2007).

    Article  CAS  Google Scholar 

  18. Castellvi, J. et al. Phosphorylated 4E binding protein 1: a hallmark of cell signaling that correlates with survival in ovarian cancer. Cancer 107, 1801–1811 (2006).

    Article  CAS  Google Scholar 

  19. Dumstorf, C.A. et al. Modulation of 4E-BP1 function as a critical determinant of enzastaurin-induced apoptosis. Mol. Cancer Ther. 9, 3158–3163 (2010).

    Article  CAS  Google Scholar 

  20. Park, M.H., Wolff, E.C. & Folk, J.E. Hypusine: its post-translational formation in eukaryotic initiation factor 5A and its potential role in cellular regulation. BioFactors 4, 95–104 (1993).

    CAS  Google Scholar 

  21. Schnier, J., Schwelberger, H.G., Smit-McBride, Z., Kang, H.A. & Hershey, J.W. Translation initiation factor 5A and its hypusine modification are essential for cell viability in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 11, 3105–3114 (1991).

    CAS  Google Scholar 

  22. Jakus, J., Wolff, E.C., Park, M.H. & Folk, J.E. Features of the spermidine-binding site of deoxyhypusine synthase as derived from inhibition studies. Effective inhibition by bis- and mono-guanylated diamines and polyamines. J. Biol. Chem. 268, 13151–13159 (1993).

    CAS  Google Scholar 

  23. Taylor, C.A. et al. Role of eIF5A in TNF-alpha-mediated apoptosis of lamina cribrosa cells. Invest. Ophthalmol. Vis. Sci. 45, 3568–3576 (2004).

    Article  Google Scholar 

  24. Li, A.L. et al. A novel eIF5A complex functions as a regulator of p53 and p53-dependent apoptosis. J. Biol. Chem. 279, 49251–49258 (2004).

    Article  CAS  Google Scholar 

  25. Clement, P.M. et al. Identification and characterization of eukaryotic initiation factor 5A-2. Eur. J. Biochem. 270, 4254–4263 (2003).

    Article  CAS  Google Scholar 

  26. Smith, L. et al. The proteomic analysis of cisplatin resistance in breast cancer cells. Oncol. Res. 16, 497–506 (2007).

    Article  CAS  Google Scholar 

  27. Crowley-Weber, C.L. et al. Development and molecular characterization of HCT-116 cell lines resistant to the tumor promoter and multiple stress-inducer, deoxycholate. Carcinogenesis 23, 2063–2080 (2002).

    Article  CAS  Google Scholar 

  28. Strey, C.W. et al. Hypoxia and reoxygenation of primary human hepatocytes induce proteome changes of glucose metabolism, oxidative protection and peroxisomal function. Int. J. Mol. Med. 26, 577–584 (2010).

    Article  CAS  Google Scholar 

  29. Møller-Sørensen, N. et al. Biology and potential clinical implications of tissue inhibitor of metalloproteinases-1 in colorectal cancer treatment. Scand. J. Gastroenterol. 43, 774–786 (2008).

    Article  Google Scholar 

  30. Nielsen, H.J. et al. Plasma TIMP-1 and CEA in detection of primary colorectal cancer: a prospective, population based study of 4509 high-risk individuals. Scand. J. Gastroenterol. 46, 60–69 (2011).

    Article  CAS  Google Scholar 

  31. Işlekel, H. et al. Matrix metalloproteinase-9,-3 and tissue inhibitor of matrix metalloproteinase-1 in colorectal cancer: relationship to clinicopathological variables. Cell Biochem. Funct. 25, 433–441 (2007).

    Article  Google Scholar 

  32. Yoo, B.C. et al. Galectin-3 stabilizes heterogeneous nuclear ribonucleoprotein Q to maintain proliferation of human colon cancer cells. Cell. Mol. Life Sci. 66, 350–364 (2009).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute, National Cancer Center, Gyeonggi, Korea

    Kyung-Hee Kim, Eun-Jin Cho, Sung-Chul Yea & Byong Chul Yoo

  2. East-West Cancer Center, College of Oriental Medicine, Daejeon University, Daejeon, Korea

    Byung-Rok Park, Chong-Kwan Cho, Yeon-Weol Lee & Hwa-Seung Yoo

  3. Cancer Research Institute, College of Medicine, Seoul National University, Seoul, Korea

    Kyung-Hee Kim

Authors
  1. Kyung-Hee Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Byung-Rok Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chong-Kwan Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yeon-Weol Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eun-Jin Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sung-Chul Yea
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Byong Chul Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hwa-Seung Yoo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hwa-Seung Yoo.

Additional information

These authors contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, KH., Park, BR., Cho, CK. et al. Proteome alteration in human colon cancer cells by the treatment of HangAmDan-B. BioChip J 5, 114–122 (2011). https://doi.org/10.1007/s13206-011-5203-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13206-011-5203-3

Keywords

Search

Navigation