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Synthesis, characterization and biological applications of mycosynthesized silver nanoparticles

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Abstract

Silver nanoparticles (AgNPs) have been known for their inhibitory and bactericidal effects. In the present study, less toxic AgNPs using Cunninghamella echinulata is reported for the first time. The obtained AgNPs were characterized using UV–Visible spectrophotometer, XRD, FT-IR, FE-SEM with EDAX and HR-TEM. AgNPs showed the maximum absorbance at 420–430 nm. The transmission electron micrograph revealed the formation of considerably uniform-sized AgNPs with an average size of 20–50 nm. The reducing and capping agents responsible for AgNP synthesis were identified by FT-IR. AgNP-incorporated cotton fabrics exhibited promising antibacterial activity against pathogenic bacteria. In addition, the in vitro cell viability of Vero cells (African green monkey kidney cells) was analyzed and the IC50 value of AgNPs was found to be 62.8 µg/mL. Taken together, these results clearly reveal less toxic AgNPs which could be exploited for various biomedical applications.

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References

  • Arun G, Eyini M, Gunasekaran P (2014) Green synthesis of silver nanoparticles using the mushroom fungus Schizophyllum commune and its biomedical applications. Biotechnol Bioprocess Eng 19:1083–1090

    Article  CAS  Google Scholar 

  • Azmath P, Baker S, Rakshith D, Satish S (2016) Mycosynthesis of silver nanoparticles bearing antibacterial activity. Saudi Pharm J 24:140–146

    Article  Google Scholar 

  • Balaji DS, Basavaraja S, Deshpande R, Mahesh DB, Prabhakar BK, Venkataraman A (2009) Extracellular biosynthesis of functionalized silver nanoparticles by strains of Cladosporium cladosporioides fungus. Colloids Surf B Biointerfaces 68:88–92

    Article  CAS  Google Scholar 

  • Balakumaran MD, Ramachandran R, Kalaichelvan PT (2015) Exploitation of endophytic fungus, Guignardia mangiferae for extracellular synthesis of silver nanoparticles and their in vitro biological activities. Microbiol Res 178:9–17

    Article  CAS  Google Scholar 

  • Balakumaran MD, Ramachandran R, Balashanmugam P, Mukeshkumar DJ, Kalaichelvan PT (2016a) Mycosynthesis of silver and gold nanoparticles: optimization, characterization and antimicrobial activity against human pathogens. Microbiol Res 182:8–20

    Article  CAS  Google Scholar 

  • Balakumaran MD, Ramachandran R, Jagadeeswari S, Kalaichelvan PT (2016b) In vitro biological properties and characterization of nanosilver coated cotton fabrics—an application for antimicrobial textile finishing. Int Biodeterior Biodegrad 107:48–55

    Article  CAS  Google Scholar 

  • Bhainsa KC, D’Souza SF (2006) Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Colloids Surf B Biointerfaces 47:160–164

    Article  CAS  Google Scholar 

  • Domsch KH, Gams W, Anderson T (1980) Compendium of Soil Fungi, vol 1. Academic Press, London, pp 1–860

    Google Scholar 

  • Fatima F, Verma SR, Pathak N, Bajpai P (2016) Extracellular mycosynthesis of silver nanoparticles and their microbicidal activity. J Glob Antimicrob Resist 7:88–92

    Article  Google Scholar 

  • Fayaz AM, Balaji K, Kalaichelvan PT, Venkatesan R (2009) Fungal based synthesis of silver nanoparticles—an effect of temperature on the size of particles. Colloids Surf B Biointerfaces 74:123–126

    Article  Google Scholar 

  • Fayaz AM, Balaji K, Girilal M, Yadav R, Kalaichelvan PT, Venketesan R (2010) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomed 6:103–109

    Article  CAS  Google Scholar 

  • Franci G, Falanga A, Galdiero S, Palomba L, Rai M, Morelli G, Galdiero M (2015) Silver nanoparticles as potential antibacterial agents. Molecules 20:8856–8874

    Article  CAS  Google Scholar 

  • Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M (2009) Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomed 5:382–386

    Article  CAS  Google Scholar 

  • Gopinath PM, Narchonai G, Dhanasekaran D, Ranjani A, Thajuddin N (2015) Mycosynthesis, characterization and antibacterial properties of AgNPs against multidrug resistant (MDR) bacterial pathogens of female infertility cases. Asian J Pharm Sci 10:138–145

    Article  Google Scholar 

  • Hamedi S, Shojaosadati SA, Shokrollahzadeh S, Hashemi-Najafabadi S (2014) Extracellular biosynthesis of silver nanoparticles using a novel and non-pathogenic fungus, Neurospora intermedia: controlled synthesis and antibacterial activity. World J Microbiol Biotechnol 30:693–704

    Article  CAS  Google Scholar 

  • Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N (2010) Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B Biointerfaces 76:50–56

    Article  CAS  Google Scholar 

  • Magdi HM, Mourad MHE, El-Aziz MMA (2014) Biosynthesis of silver nanoparticles using fungi and biological evaluation of mycosynthesized silver nanoparticles. Egypt J Exp Biol (Bot) 10:1–12

    Google Scholar 

  • Maliszewska I, Juraszek A, Bielska K (2014) Green synthesis and characterization of silver nanoparticles using ascomycota fungi Penicillium nalgiovense AJ12. J Clust Sci 25:989–1004

    Article  CAS  Google Scholar 

  • Mosselhy DA, El-Aziz MA, Hanna M, Ahmed MA, Husien MM, Feng Q (2015) Comparative synthesis and antimicrobial action of silver nanoparticles and silver nitrate. J Nanopart Res 17:473

    Article  Google Scholar 

  • Nayak RR, Pradhan N, Behera D, Pradhan KM, Mishra S, Sukla LB, Mishra BK (2011) Green synthesis of silver nanoparticle by Penicillium purpurogenum NPMF: the process and optimization. J Nanoparticle Res 13:3129–3137

    Article  CAS  Google Scholar 

  • Perelshtein I, Applerot G, Perkas N, Guibert G, Mikhailov S, Gedanken A (2008) Sonochemical coating of silver nanoparticles on textile fabrics (nylon, polyester and cotton) and their antibacterial activity. Nanotechnology 19:245705

    Article  Google Scholar 

  • Saravana Kumar P, Balachandran C, Duraipandiyan V, Ramasamy D, Ignacimuthu S, Al-Dhabi NA (2015) Extracellular biosynthesis of silver nanoparticle using Streptomyces sp. 09 PBT 005 and its antibacterial and cytotoxic properties. Appl. Nanosci 5:169–180

    Article  CAS  Google Scholar 

  • Saxena R, Mehrotra BS (1952) Fungus flora of Allahabad soil. Proc nat Acad India 22:22–43

    Google Scholar 

  • Velhal SG, Kulkarni SD, Latpate RV (2016) Fungal mediated silver nanoparticle synthesis using robust experimental design and its application in cotton fabric. Int Nano Lett 6:257–264

    Article  CAS  Google Scholar 

  • Yadav A, Kon K, Kratosova G, Duran N, Ingle AP, Rai M (2015) Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research. Biotechnol Lett 37:2099–2120

    Article  CAS  Google Scholar 

  • Zhang G, Liu Y, Gao X, Chen Y (2014) Synthesis of silver nanoparticles and antibacterial property of silk fabrics treated by silver nanoparticles. Nanoscale Res Lett 9:216

    Article  Google Scholar 

Download references

Acknowledgements

The author thanks the University Grants Commission (UGC), New Delhi, India for providing research fellowship under UGC-BSR scheme. We are also grateful to the Director, CAS in Botany, University of Madras for providing laboratory facilities. The authors are thankful to the Director, National Centre for Nanoscience and Nanotechnology (NCNSNT), University of Madras for electron microscopic facilities.

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Authors and Affiliations

  1. Nanobiotechnology Lab, Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, 600025, India

    Sathiyaseelan Anbazhagan, Shajahan Azeez, Girilal Morukattu, Ramachandran Rajan, Kaviyarasan Venkatesan & Kalaichelvan Puthupalayam Thangavelu

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  1. Sathiyaseelan Anbazhagan
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  2. Shajahan Azeez
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  3. Girilal Morukattu
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  4. Ramachandran Rajan
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  5. Kaviyarasan Venkatesan
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  6. Kalaichelvan Puthupalayam Thangavelu
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Correspondence to Sathiyaseelan Anbazhagan.

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Anbazhagan, S., Azeez, S., Morukattu, G. et al. Synthesis, characterization and biological applications of mycosynthesized silver nanoparticles. 3 Biotech 7, 333 (2017). https://doi.org/10.1007/s13205-017-0961-9

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