Abstract
Most of the health benefits of fish oil can be attributed to the presence of omega-3 fatty acids like Docosahexenoic acid (DHA) and Eicosapentaenoic acid (EPA). There are few dietary sources of EPA and DHA other than oily fish. EPA and DHA have great potential effect on human health. In this research, Supercritical carbon dioxide (scCO2) extracted mackerel oil was reacted by enzyme at different systems to improve the EPA and DHA. Different types of immobilize enzyme TL-IM, RM-IM, Novozyme 435 were assessed for improving PUFAs. Best result was found at non-pressurized system using TL-IM. Reacted oil particle were obtained with polyethylene glycol by gas saturated solution process (PGSS). Different parameters like temperature, pressure, agitation speed and nozzle size effect on particle formulation were observed. SEM and PSA analysis showed, small size non spherical particles were obtained. It was found that after particle formation poly unsaturated fatty acids (PUFAs) were present in particle as same in oil. PUFAs release from particle was almost linear against constant time duration. Oil quality in particle not change significantly, in this contrast this study will be helpful for food and pharmaceutical industry to provide high EPA and DHA containing powder.
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References
American Oil Chemists’ Society (AOCS) (1998) Official methods of analysis. 6th edn. American Oil Chemists’Society,Champaign.
Bernardez M, Pastoriza L, Sampedro G, Herrera JJR, Cabo ML (2005) Modified method for the analysis of free fatty acids in fish. Agric Food Chem 53(6):1903–1906
Budavari S (1989) An encyclopedia of chemicals, drugs and biological, 11th edn. The Merck Index, Merck and Co. Inc, Rahway, New Jersey
Cao XJ, Hur BK (2005) Separation of EPA and DHA from fish oil using modified zeolite 13× and supercritical CO2. J Ind Eng Chem 11:762–768
Cocero MJ, Martin A, Mattea F, Varona S (2009) Encapsulation and co-precipitation processes with supercritical fluids: fundamentals and applications. J Supercrit Fluids 47:546–555
Correa APA, Peixoto CA, Goncalves LAG, Cabral FA (2008) Fractionation of fish oil with supercritical carbon dioxide. J Food Eng 88:381–387
Essien EA, Umoren SA, Essien EE, Udoh AP (2012) Preparation and evaluation of cucumeropsis mannii naud seed oil metallic soaps as driers in gloss paint. J Mater Environ Sci 3(3): 477–484
Green P, Hermesh H, Monselise A, Marom S, Presburger G, Weizman A (2006) Red cell membrane omega-3 fatty acids are decreased in nondepressed patients with social anxiety disorder. Eur Neuropsychopharmacol 16(2):107–113
Gunnlaugsdottir H, Wannerberger K, Sivik B (1998) Alcoholysis and glyceride synthesis with immobilized lipase on controlled-pore glass of varying hydrophobicity in supercritical carbon dioxide. Enzym Microb Technol 22:360–367
Jeong YS, Song SK, Lee SJ, Hur BK (2006) The growth and EPA synthesis of shewanella oneidensis MR-1 and expectation of EPA biosynthetic pathway. Biotechnol Bioprocess Eng 11:127–133
Kadic A, Palmqvist B, Liden G (2014) Effects of agitation on particle-size distribution and enzymatic hydrolysis of pretreated spruce and giant reed. Biotechnol Biofuels 7:77. doi:10.1186/1754-6834-7-77
Kamal-Eldin A, Yanishlieva NV (2002) ω-3 fatty acid for human nutrition: stability considerations. Eur J Lipid Sci Technol 104(12):825–836
Knez Z, Habulin M (2002) Compressed gases as alternative enzymatic-reaction solvents: a short review. J Supercrit Fluids 23:29–42
Lanna AC, Jose IC, Oliveira MGA, Barros EG, Moreira MA (2005) Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds. Braz J Plant Physiol 17(2):213–222
Lee SJ, Jeong YS, Kim DU, Seo JW, Hur BK (2006) Eicosapentaenoic acid (EPA) biosynthetic gene cluster of shewanella oneidensis MR-1: cloning, heterologous expression and effects of temperature and glucose on the production of EPA in Escherichia coli. Biotechnol Bioprocess Eng 11:510–515
Lozano P, Villora G, Gomez D, Gayo AB, Sanchez-Conesa JA, Rubio M, Iborra JL (2004) Membrane reactor with immobilised Candica antartica lipase B for ester synthesis in supercritical carbon dioxide. J Supercrit Fluids 29:121–128
Martı’n Valverde L, Gonza’lez Moreno PA, Rodrı’guez Quevedo A, Hita Peña E, Jime’nez Callejo’n, MJ, Esteban Cerda’n L, Molina Grima E, Robles Medina A (2012) Concentration of docosahexaenoic acid (DHA) by selective alcoholysis catalyzed by lipases. J Am Oil Chem Soc 89: 1633–1645
Martin A, Cocero MJ (2008) Micronization process with supercritical fluids: fundamentals and mechanisms. Adv Drug Deliv Rev 60:339–350
Martín Á, Pham HM, Kilzer A, Kareth S, Weidner E (2010) Micronization of polyethylene glycol by PGSS (particle from gas saturated solutions)-drying of aqueous solutions. Chem Eng Process Process Intensif 49:1259–1266
Nakaya H, Miyawaki O, Nakamura K (2001) Determination of log P for pressurized carbon dioxide and its characterization as a medium for enzyme reaction. Enzym Microb Technol 28(2–3):176–182
Nalawade SP, Picchioni F, Janssen LPBM (2006) Supercritical carbon dioxide as a green solvent for processing polymer melts: processing aspects and applications. Prog Polym Sci 31:19–43
Naliwaiko K, Araújo RLF, Da Fonseca RV, Castilho JC, Andreatini R, Bellissimo MI, Oliveira BH, Martins EF, Curi R, Fernandes LC, Ferraz AC (2004) Effects of fish oil on the central nervous system: a new potential antidepressant. Nutr Neurosci 7(2):91–99
Nemets B, Stahl Z, Belmaker RH (2002) Addition of omega-3 fatty acid to maintenance medication treatment for recurrent unipolar depressive disorder. Am J Psychiatry 159(3):477–479
Okada H (1997) One- and three-month release injectable microspheres of the LH–RH superagonist leuprorelin acetate. Adv Drug Deliv Rev 28(1):43–70
Oliveira JV, Oliveira D (2000) Kinetics of enzymatic alcoholysis of palm kernel oil in SC-CO2. Ind Eng Chem Res 39:4450–4454
Oliveira D, Feihrmann AC, Rubira AF, Kunita MH, Dariva C, Oliveira JV (2006) Assessment of two immobilized lipases activity treated in compressed fluids. J Supercrit Fluids 38:373–382
Osman H, Suriah AR, Law EC (2001) Fatty acid composition and cholesterol content of selected marine fish in Malaysian waters. J Food Chem 73:55–60
Savage PE, Gopalan S, Mizan TL, Martino CJ, Brock EE (1995) Reactions at supercritical conditions–applications and fundamentals. J AIChE 41(7):1723–1778
Su KP, Huang SY, Chiu CC, Shen WW (2003) Omega-3 fatty acids in major depressive disorder. Eur Neuropsychopharmacol 13(4):267–271
Tracy MA (1998) Development and scale-up of a microsphere protein delivery system. Biotechnol Prog 14(1):108–115
Yamaguchi I, Akoh CC, Lai OM (2004) Modification of fish oil by lipozyme TL IM to produce structured lipid. J Food Lipids 11:65–73
Yehuda S, Rabinovitz S, Mostofsky DI (2005) Mixture of essential fatty acids lowers test anxiety. Nutr Neurosci 8(4):265–267
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This work was supported by a Research Grant of Pukyong National University (2015).
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Research Highlights
• Supercritical CO2 extracted mackerel oil was used
• 3 different immobilized lipase enzymes were used to improve polyunsaturated fatty acid.
• Gas saturated solution (PGSS) process was used for particle formation
• Different parameters were observed for optimization in PGSS process
• Control release were observed of PUFAs from particle
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Tanbirul Haque, A.S.M., Chun, BS. Particle formation and characterization of mackerel reaction oil by gas saturated solution process. J Food Sci Technol 53, 293–303 (2016). https://doi.org/10.1007/s13197-015-2000-3
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DOI: https://doi.org/10.1007/s13197-015-2000-3