Abstract
Vibrio vulnificus is an opportunistic pathogen widely distributed in estuarine and coastal seawaters. In this study, a culture-free method was developed to rapid detection of V. vulnificus in all seasons, based on loop-mediated isothermal amplification (LAMP) targeting virulent-correlated gene (vcg). The new assay method allows differentiation between the virulent and non-virulent strain of V. vulnificus accurately. This method also allows effective detection of the pathogeny in winter when the bacterium lives in the viable but non-culturable (VBNC) state. A total of 30 costal seawater samples collected in all seasons were used for the evaluation of this method. The results show that the method is sensitive, accurate and convenient.
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Foundation item: The National High-Tech Research and Development Program of China (“863”) under contract No.2006AA09Z170.
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Li, Y., Zheng, Z., Zhao, Y. et al. A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene. Acta Oceanol. Sin. 29, 93–97 (2010). https://doi.org/10.1007/s13131-010-0027-x
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DOI: https://doi.org/10.1007/s13131-010-0027-x