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Development of 14 microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus) using next generation sequencing technology

  • Microsatellite Letters
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Abstract

We report the development of 14 new microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus). Ten unrelated Queensland koala individuals from the San Diego Zoo, USA, were genotyped. The number of alleles per locus ranged from 2 to 7, with an average of 5.14 alleles per locus. Across all loci, the average observed and expected heterozygosity values were both 0.69. These polymorphic microsatellite loci will be useful for genetic studies relevant to the conservation of the koala, a species listed as vulnerable.

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References

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Acknowledgments

For generously providing koala samples, we thank the San Diego Zoo, Columbus Zoo, San Francisco Zoo, and Dallas Zoo. We thank K. Zhao for modifying the MSATCOMMANDER program and J. R. Brandt for creating a PERL script to search primer sequences against the 454 sequence database. We thank the UIUC Core Sequencing Facility for fragment analysis. The project described was supported by grant number R01GM092706 from the National Institute of General Medical Sciences (NIGMS). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIGMS or the National Institutes of Health.

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Correspondence to Alfred L. Roca.

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Ruiz-Rodriguez, C.T., Ishida, Y., Greenwood, A.D. et al. Development of 14 microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus) using next generation sequencing technology. Conservation Genet Resour 6, 429–431 (2014). https://doi.org/10.1007/s12686-013-0115-2

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  • DOI: https://doi.org/10.1007/s12686-013-0115-2

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