Abstract
The infection of human parvovirus B19 (B19V) is a common event in the general population, including volunteer blood donors. In some cases it can be asymptomatic and can remain persistent for a long period of time. The objective of this study was to examine the B19V DNA prevalence and viral load in first-time volunteer blood donors. Blood samples were collected from 91 primary blood donors at the Regional Blood Center of Ribeirão Preto, Southeast Brazil. Viral detection and quantitation was performed by an in-house TaqMan® real-time PCR with high sensitivity. B19V DNA was detected in one male blood donor (1.0 %) and was characterized by a very low viral load (537.36 copies/mL). Our studies demonstrate that B19V DNA at low titer may be present in apparently healthy individuals. Sensitive molecular diagnostic tools can be applied for the screening of fresh blood derived products in order to prevent transfusion-transmitted B19V infection.
References
Heegaard ED, Brown KE (2002) Human parvovirus B19. Clin Microbiol Rev 15:485–505
Slavov SN, Haddad SK, Silva-Pinto AC, Amarilla AA, Alfonso HL, Aquino VH, Covas DT (2012) Molecular and phylogenetic analyses of human parvovirus B19 isolated from Brazilian patients with sickle Cell disease, beta-thalassemia major and healthy blood donors. J Med Virol 84:1652–1665
Juhl D, Steppat D, Görg S, Hennig H (2014) Parvovirus B19 infections and blood counts in blood donors. Transfus Med Hemother 41:52–59
Corcioli F, Zakrzewska K, Rinieri A, Fanci R, Innocenti M, Civinini R, De Giorgi V, Di Lollo S, Azzi A (2008) Tissue persistance of p23-3arvovirus B19 genotypes in asymptomatic persons. J Med Virol 80:2005–2011
Lefrère JJ, Servant-Delmas A, Candotti D, Mariotti M, Thomas I, Brossard Y, Lefrère F, Girot R, Allain JP, Laperche S (2005) Persistent B19 infection in immunocompetent individuals: implications for transfusion safety. Blood 106:2890–2895
Matsukura H, Shibata S, Tani Y, Shibata H, Furuta RA (2008) Persistent infection by human parvovirus B19 in qualified blood donors. Transfusion 48:1036–1037
Corcoran A, Doyle S (2004) Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19. J Med Microbiol 53:459–475
Kleinman SH, Glynn SA, Lee TH, Tobler L, Montalvo L, Todd D, Kiss JE, Shyamala V, Busch MP, National Heart, Lung, Blood Institute, Retrovirus Epidemiology Donor Study (REDS-II) (2007) Prevalence and quantitation of parvovirus B19 DNA levels in blood donors with a sensitive polymerase chain reaction screening assay. Transfusion 47:1756–1764
Thomas I, Di Giambattista M, Gérard C, Mathys E, Hougardy V, Latour B, Branckaert T, Laub R (2003) Prevalence of human erythrovirus B19 DNA in healthy Belgian blood donors and correlation with specific antibodies against structural and non-structural viral proteins. Vox Sang 84:300–307
Ke L, He M, Li C, Liu Y, Gao L, Yao F, Li J, Bi X, Lv Y, Wang J, Hirsch ML, Li W (2011) The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers. Transfusion 51:1909–1918
Kooistra K, Mesman HJ, de Waal M, Koppelman MH, Zaaijer HL (2011) Epidemiology of high-level parvovirus B19V viraemia among Dutch blood donors, 2003–2009. Vox Sang 100(3):261–266
Juhl D, Görg S, Hennig H (2014) Persistence of Parvovirus B19 (B19V) DNA and humoral immune response in B19V-infected blood donors. Vox Sang 107:226–232
Lee TH, Kleinman SH, Wen L, Montalvo L, Todd DS, Wright DJ, Tobler LH, Busch MP, NHLBI, Retrovirus Epidemiology Donor Study-II (REDS-II) (2011) Distribution of parvovirus B19 DNA in blood compartments and persistence of virus in blood donors. Transfusion 51:1896–1908
US Department of Health and Human Services, Food and Drug Administration, Center for Biologics Evaluation and Research. (2009) Nucleic Acid Testing (NAT) to Reduce the Possible Risk of Human Parvovirus B19 Transmission by Plasma-Derived Products. Available via: http://www.fda.gov/downloads/BiologicsBloodVaccines/GuidanceComplianceRegulatoryInformation/Guidances/Blood/ucm078510.pdf
Tsitsikas DA, Gallinella G, Patel S, Seligman H, Greaves P, Amos RJ (2014) Bone marrow necrosis and fat embolism syndrome in sickle cell disease: increased susceptibility of patients with non-SS genotypes and a possible association with human parvovirus B19 infection. Blood Rev 28:23–30
Acknowledgements
The study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, Brazil (Grants No. 2009/16623-1, CTC-1998/14.247-6 and INCTC-2008/57.877-3), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (INCTC-573.754/2008-0).
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Slavov, S.N., Otaguiri, K.K., Covas, D.T. et al. Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil. Indian J Hematol Blood Transfus 32 (Suppl 1), 323–325 (2016). https://doi.org/10.1007/s12288-015-0607-1
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DOI: https://doi.org/10.1007/s12288-015-0607-1