Skip to main content
SpringerLink
Log in

Enhanced production of heteropolysaccharide-7 by Beijerinckia indica HS-2001 in pilot-scaled bioreactor under optimized conditions involved in dissolved oxygen using sucrose-based medium

  • Research Paper
  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Heteropolysaccharide-7 (PS-7) is a possible alternative to xanthan or gellan used as food additives due to its properties and potential applications. Sucrose was developed as a carbon source for production of PS-7 by Beijerinckia indica HS-2001 to overcome catabolite repression against glucose. The inoculum size was established to be 5.0% (v/v) for the pilot-scaled production of PS-7 by B. indica HS-2001 based on productivity and economic aspect. The optimal agitation speed and aeration rate for cell growth of B. indica HS-2001 were found to be 495 rpm and 1.8 vvm using response surface methodology (RSM), whereas those for production of PS-7 were 440 rpm and 1.2 vvm. The optimal inner pressure for cell growth of B. indica HS-2001 in a 100 L bioreactor was 0.02 MPa, whereas that for production of PS-7 was 0.04 MPa. The production of PS-7 by B. indica HS-2001 from 30.0 g/L sucrose under an optimized inner pressure in a 100 L bioreactor was 10.20 g/L, which was 1.32 times higher than that without inner pressure. The maximal production of PS-7 under optimal conditions involved in the dissolved oxygen using the sucrose-based medium developed in this study was 1.55 times higher than that before optimization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Jin, H., H. S. Kim, S. K. Kim, M. K. Shin, J. H. Kim, and J. W. Lee (2002) Production of heteropolysaccharide-7 by Beijerinckia indica from agro-industrial byproducts. Enz. Microb. Technol. 30: 822–827.

    Article  CAS  Google Scholar 

  2. Baup, A. A. S., N. Gondrexon, J. Magnin, and J. Willison (2011) Enhancement of mass transfer characteristics and phenanthrene degradation in a two-pahse partitioning bioreactor equipped with internal static mixers. Biotechnol. Bioproc. Eng. 16: 413–418.

    Article  Google Scholar 

  3. Kang, K. S. and W. H. McNeely (1976) Polysaccharide and bacterial fermentation process for its preparation. US Patent 3,960,832.

    Google Scholar 

  4. Lee, J. W., W. G. Yeoman, A. L. Allen, R. A. Gross, and D. L. Kaplan (1997) Compositional consistency of a heteropolysaccharide-7 produced by Beijerichia indica. Biotechnol. Lett. 19: 803–807.

    Article  CAS  Google Scholar 

  5. Palaniraj, A. and V. Jayaraman (2011) Production, recovery and applications of xanthan gum by Xanthamonas campestris. J. Food Eng. 106: 1–12.

    Article  CAS  Google Scholar 

  6. Shah, N., J. H. Ha, and J. K. Park (2010) Effect of reactor surface on production of bacterial cellulose and water soluble oligosaccharides by Gluconacetobacter bansenii PJK. Biotechnol. Bioproc. Eng. 15: 110–118.

    Article  CAS  Google Scholar 

  7. Chen, Y., C. J. Lin, H. Lan, S. Fu, and H. Zhan (2010) Evaluation of kinetic parameters and mass transfer of glucose-fed granules under hypoxic conditions. Biotechnol. Bioproc. Eng. 15: 931–936.

    Article  CAS  Google Scholar 

  8. Jin, H., J. K. Yang, K. I. Jo, C. H. Chung, S. K. Kim, S. W. Nam, and J. W. Lee (2006) Mass production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica HS-2001 with soybean pomace as a nitrogen source. Proc. Biochem. 41: 270–275.

    Article  CAS  Google Scholar 

  9. González-Sáiz, J., D. Garrido-Vidal, and C. Pisarro (2009) Modeling the industrial production of vinegar in aerated-stirred fermentors in terms of process variables. J. Food Eng. 91: 183–196.

    Article  Google Scholar 

  10. Giavasis, I., L. M. Harvey, and B. McNeil (2006) The effect of agitation and aeration on the synthesis and molecular weight of gellan in batch cultures of Sphingmonas paucimobilis. Enz. Microb. Technol. 38: 101–108.

    Article  CAS  Google Scholar 

  11. Thiry, M. and D. Cinogolani (2002) Optimizing scale-up fermentation process. Trend. Biotechnol. 20: 103–105.

    Article  CAS  Google Scholar 

  12. Junker, B. H. (2004) Scale-up methodologies for Escherichia coli and yeast fermentation process. J. Biosci. Bioeng. 97: 347–364.

    CAS  Google Scholar 

  13. Gao, W., Y. J. Kim, C. H. Chung, J. Li, and J. W. Lee (2010) Optimization of mineral salts in medium for enhanced production of pullulan by Aureobasidium pullulans HP-2001 using an orthogonal array method. Biotechnol. Bioproc. Eng. 15: 837–845.

    Article  CAS  Google Scholar 

  14. Gao, W., C. H. Chung, J. Li, and J. W. Lee (2011) Application of statistical experimental design for optimization of physiological factors and their influences on production of pullulan by Aureobasidium pullulans HP-2001 using an orthogonal array method. Kor. J. Chem. Eng. 28: 2184–2189.

    Article  CAS  Google Scholar 

  15. Lee, Y. J., H. J. Kim, W. Gao, C. H. Chung, and J. W. Lee (2012) Statistical optimization for production of carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a recombinant Escherichia coli JM109/DL-3 from rice bran using response surface method. Biotechnol. Bioproc. Eng. 17: 227–235.

    Article  CAS  Google Scholar 

  16. Gassara, F., S. K. Brar, R. D. Tyagi, R. P. John, M. Verma, and J. R. Valero (2011) Parameter optimization for production of ligninolytic enzymes using agro-industrial wastes by response surface method. Biotechnol. Bioproc. Eng. 16: 343–351.

    Article  CAS  Google Scholar 

  17. Kim, H. J., Y. J. Lee, W. Gao, C. H. Chung, and J. W. Lee (2012) Optimization of salts in medium for production of carboxymethylcellulase by a psychrophilic marine bacterium, Psychrobacter aquimaris LBH-10 using two statistical methods. Kor. J. Chem. Eng. 29: 384–391.

    Article  CAS  Google Scholar 

  18. Wu, J. R., J. H. Son, H. J. Seo, K. H. Kim, Y. K. Nam, J. W. Lee, and S. K. Kim (2005) Metabolic flux analysis of Beijerinckia indica for PS-7 production. Biotechnol. Bioproc. Eng. 10: 91–98.

    Article  CAS  Google Scholar 

  19. Jin, I. H., D. Y. Jung, C. W. Son, S. K. Kim, W. Gao, C. H. Chung, and J. W. Lee (2011) Enhanced production of heteropolysaccharide-7 in repeated batch culture with optimized substitution of culture medium. Biotechnol. Bioproc. Eng. 16: 245–255.

    Article  CAS  Google Scholar 

  20. Oliverira, M. R., R. S. S. F. Silva, J. B. Buzato, and M. A. P. C. Celligoi (2007) Study of levan production by Zymomans mobilis using regional low-cost carbohydrate sources. Biochem. Eng. J. 37: 177–183.

    Article  Google Scholar 

  21. Survase, S. A., P. S. Saudagar, and R. S. Singhal (2006) Production of scleroglucan from Sclerotium rolfsii MTCC 2156. Bioresour. Technol. 97: 989–993.

    Article  CAS  Google Scholar 

  22. Seo, H. P., C. H. Chung, S. K. Kim, R. A. Gross, D. L. Kaplan, and J. W. Lee (2004) Mass production of pullulan with optimized concentrations of carbon and nitrogen sources by Aureobaisdium pullulans HP-2001 in a 100 L bioreactor with the inner pressure. J. Microbiol. Biotechnol. 14: 237–242.

    CAS  Google Scholar 

  23. Seo, H. P., K. I. Jo, C. W. Son, J. K. Yang, C. H. Chung, S. W. Nam, S. K. Kim, and J. W. Lee (2006) Continuous production of pullulan by Aureobasidium pullulans HP-2001 with feeding of high concentration of sucrose. J. Microbiol. Biotechnol. 16: 374–380.

    CAS  Google Scholar 

  24. Vaheed, H., S. A. Shojaosadati, and H. Galip (2011) Evaluation and optimization of ethanol production from carob pod extract by Zymomonas mobilis using response surface methodology. J. Ind. Microbiol. Biotechnol. 38: 101–111.

    Article  CAS  Google Scholar 

  25. Loc, N. H. and N. T. T. An (2010) Asiaticoside production from centella (Centella asistica L. Urban) cell culture. Biotechnol. Bioproc. Eng. 15: 1065–1070.

    Article  Google Scholar 

  26. Nampoothiri, K. M., R. R. Singhania, C. Sabarinath, and A. Pandey (2003) Fermentative production of gellan using Sphingomo nas paucimobilis. Proc. Biochem. 38: 1513–1519.

    Article  CAS  Google Scholar 

  27. Niladevi, K. N., R. K. Sukumaran, and P. Prema (2007) Utilization of rice straw for laccase production by Streptomyces psammoticus in solid-state fermentation. J. Ind. Microbiol. Biotechnol. 34: 665–674.

    Article  CAS  Google Scholar 

  28. Charoenrat, T., M. Ketudat-Cairns, M. Jahic, A. Veide, and S. Enfors (2006) Increased total air pressure versus oxygen limitation for enhanced oxygen transfer and product formation in a Pichia pastoris recombinant protein process. Biochem. Eng. J. 30: 205–211.

    Article  CAS  Google Scholar 

  29. Papagianni, M., S. K. Psomas, L. Batsilas, S. V. Paras, D. A. Kyriakidis, and M. Liakopoulou-Kyriakides (2001) Xanthan production by Xanthomonas campestris in batch cultures. Proc. Biochem. 37: 73–80.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Korea Bio-Solution Co., Busan, 612-020, Korea

    Dae-Young Jung & Chang-Woo Son

  2. Department of Biotechnology, Pukyung National University, Busan, 608-737, Korea

    Sung-Koo Kim

  3. BK21 Bio-Silver Program of Dong-A University, Busan, 604-714, Korea

    Wa Gao & Jin-Woo Lee

  4. Department of Biotechnology, Dong-A University, Busan, 604-714, Korea

    Jin-Woo Lee

Authors
  1. Dae-Young Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chang-Woo Son
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sung-Koo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wa Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jin-Woo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Woo Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jung, DY., Son, CW., Kim, SK. et al. Enhanced production of heteropolysaccharide-7 by Beijerinckia indica HS-2001 in pilot-scaled bioreactor under optimized conditions involved in dissolved oxygen using sucrose-based medium. Biotechnol Bioproc E 18, 94–103 (2013). https://doi.org/10.1007/s12257-012-0520-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-012-0520-5

Keywords

Search

Navigation