Abstract
Imbalance between neutrophil elastase and alpha-1-antitrypsin (AAT) leads to emphysema in smokers as well as in patients with inherited alpha-1-antitrypsin deficiency. AAT as a proven inhibitor of apoptosis may play role in lung cancer (LC) progression. The aim was to analyse AAT protein variants and polymorphism in promoter region of the neutrophil elastase gene (ELA2) in patients with primary lung cancer. AAT phenotypisation by isoelectric focusing method and ELA2 gene promoter characterization by DNA sequencing were performed in 66 patients with primary lung cancer. Results showed that the frequency of M1 allele and PiM1 homozygotes in LC patients was significantly higher when compared to the healthy subjects (f = 0.6360 and 0.7424 respectively). The most frequent ELA2 promoter region genotypes in LC patients were −903TT and −741GG. There were significantly more patients with intermediate and high ELA2 genotype activity, compared to those with low activity (91% vs. 9%, respectively). In conclusion, we found that PiM1 homozygosity could be associated with the lung cancer, probably due to increased synthesis of this antiapoptotic protein. Non-MM variants of AAT and ELA2 genotypes with predicted intermediate or high activity could also represent a risk factor for aggressive form of lung cancer associated with extrathoracic metastases.
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References
Cancer Research UK (2005) Commonly diagnosed cancers worldwide. Retrieved on 2008-01-11. http://info.cancerresearchuk.org/cancerstats/world/index.htm
Mattson ME, Pollack ES, Cullen JW (1987) What are the odds that smoking will kill you? Am J Public Health 77:425–431
Bach PB, Kattan MW, Thornquist MD, Kris MG, Tate RC, Barnett MJ, Hsieh LJ, Begg CB (2003) Variations in lung cancer risk among smokers. J Natl Cancer Inst 95:470–478
Yao H, Rahman I (2009) Current concepts on the role of inflammation in COPD and lung cancer. Curr Opin Pharmacol 9:375–383
Carp H, Miller F, Hoidal JR, Janoff A (1982) Potential mechanism of emphysema: alpha 1-proteinase inhibitor recovered from lungs of cigarette smokers contains oxidized methionine and has decreased elastase inhibitory capacity. Proc Natl Acad Sci USA 79:2041–2045
Taggart C, Cervantes-Laurean D, Kim G, McElvaney NG, Wehr N, Moss J, Levine RL (2000) Oxidation of either methionine 351 or methionine 358 in alpha 1-antitrypsin causes loss of anti-neutrophil elastase activity. J Biol Chem 75:27258–27265
Walser T, Cui X, Yanagawa J, Lee JM, Heinrich E, Lee G, Sharma S, Dubinett SM (2008) Smoking and lung cancer: the role of inflammation. Proc Am Thorac Soc 5:811–815
Petrache I, Fijalkowska I, Zhen L, Medler TR, Brown E, Cruz P, Choe KH, Taraseviciene-Stewart L, Scerbavicius R, Shapiro L, Zhang B, Song S, Hicklin D, Voelkel NF, Flotte T, Tuder RM (2006) A novel antiapoptotic role for α1-antitrypsin in the prevention of pulmonary emphysema. Am J Respir Crit Care Med 173:1222–1228
Zimmer M, Medcalf RL, Fink TM, Mattmann C, Lichter P, Jenne DE (1992) Three human elastase-like genes coordinately expressed in myelomonocytic lineage are organized as a single genetic locus on 19pter. Proc Natl Acad Sci 89:8215–8223
Takahashi H, Nukiwa T, Basset P, Crystal RG (1988) Myelomonocytic cell lineage expression of the neutrophil elastase gene. J Biol Chem 263:2543–2547
Kostmann R (1956) Infantile genetic agranulocytosis. Acta Paediatr 45(suppl 105):1–78
Ancliff PJ, Gale RE, Liesner R, Hann IM, Linch DC (2001) Mutations in the ELA2 gene encoding neutrophil elastase are present in most patients with sporadic severe congenital neutropenia but only in some patients with the familial form of the disease. Blood 98:2645–2650
Yamashita J, Tashiro K, Yoneda S, Kawahara K, Shirakusa T (1996) Local increase in polymorphonuclear leukocyte elastase is associated with tumor invasiveness in non-small cell lung cancer. Chest 109:1328–1334
Taniguchi K, Yang P, Jett J, Bass E, Meyer R, Wang Y, Deschamps C, Liu W (2002) Polymorphisms in the promoter region of the neutrophil elastase gene are associated with lung cancer development. Clin Cancer Res 8:1115–1120
Park JY, Chen L, Lee J, Sellers T, Tockman MS (2005) Polymorphisms in the promoter region of neutrophil elastase gene and lung cancer risk. Lung Cancer 48:315–321
Topic A, Juranic Z, Jelic S, Magazinovic Golubicic I (2009) Polymorphism of alpha-1-antitrypsin in haematological malignancies. Genet Mol Biol 32:716–719
Kishimoto Y, Yamada S, Hirayama C (1990) An association between AAT phenotype and chronic liver disease. Hum Genet 84:132–136
Topic AS, Jelic-Ivanovic ZD, Spasojevic-Kalimanovska VV, Spasic SM (2006) Association of moderate alpha-1-antitrypsin deficiency with lung cancer in the Serbian population. Arch Med Res 37:866–870
Yang P, Wentzlaff KA, Katzmann JA, Marks RS, Allen MS, Lesnick TG, Lindor NM, Myers JL, Wiegert E, Midthun DE, Thibodeau SN, Krowka MJ (1999) Alpha1-antitrypsin deficiency allele carriers among LC patients. Cancer Epidemiol Biomarkers Prev 8:461–465
Pott GB, Chan ED, Dinarello CA, Shapiro L (2009) α-1-Antitrypsin is an endogenous inhibitor of proinflammatory cytokine production in whole blood. J Leukoc Biol 85:886–895
Hadzic R, Nita I, Tassidis H, Riesbeck K, Wingren AG, Janciauskiene S (2006) α1-Antitrypsin inhibits Moraxella catarrhalis MID protein-induced tonsillar B cell proliferation and IL-6 release. Immunol Lett 102:141–147
Molano RD, Pileggi A, Song S, Zahr E, Jose SS, Molina J, Fort A, Wasserfall C, Ricordi C, Atkinson MA, Inverardi L (2008) Prolonged islet allograft survival by alpha-1 antitrypsin: the role of humoral immunity. Transplant Proc 40:455–456
Churg A, Dai J, Zay K, Karsan A, Hendricks R, Yee C, Martin R, MacKenzie R, Xie C, Zhang L, Shapiro S, Wright JL (2001) Alpha-1-antitrypsin and a broad spectrum metalloprotease inhibitor, RS113456, have similar acute anti-inflammatory effects. Lab Invest 81:1119–1131
Petrache I, Fijalkowska I, Medler TR, Skirball J, Cruz P, Zhen L, Petrache HI, Flotte TR, Tuder RM (2006) α-1 antitrypsin inhibits caspase-3 activity, preventing lung endothelial cell apoptosis. Am J Pathol 169:1155–1166
Maneckjee R, Minna JD (1994) Opioids induce while nicotine suppresses apoptosis in human lung cancer cells. Cell Growth Differ 5:1033–1040
Yamashita J, Ogawa M, Abe M, Hayashi N, Kurusu Y, Kawahara K, Shirakusa T (1997) Tumor neutrophil elastase is closely associated with the direct extension of non-small cell lung cancer into the aorta. Chest 111:885–890
Yang P, Bamlet WR, Sun Z, Ebbert JO, Aubry MC, Taylor WR, Marks RS, Deschamps C, Swensen SJ, Wieben ED, Cunningham JM, Melton LJ, de Andrade M (2005) α1-antitrypsin and neutrophil elastase imbalance and lung cancer risk. Chest 128:445–452
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This work was supported by grants 145036 and 143051 from the Ministry of Science and Environmental Protection, Republic of Serbia.
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The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.
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Topic, A., Ljujic, M., Nikolic, A. et al. Alpha-1-antitrypsin Phenotypes and Neutrophil Elastase Gene Promoter Polymorphisms in Lung Cancer. Pathol. Oncol. Res. 17, 75–80 (2011). https://doi.org/10.1007/s12253-010-9283-5
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DOI: https://doi.org/10.1007/s12253-010-9283-5