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Rhabdomyosarcoma Subtyping by Immunohistochemical Assessment of Myogenin: Tissue Array Study and Review of the Literature

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Pathology & Oncology Research

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Abstract

Myogenin immunostaining has been described as a useful marker of the alveolar subtype of rhabdomyosarcoma and as a tool for distinguishing it from the more common embryonal subtype. To add to the growing body of literature describing this phenomenon we analysed myogenin immunohistochemical staining in 152 tumors using a rhabdomyosarcoma tissue array. Results were analysed blinded to histological type by two independent investigators. Samples were excluded if any samples failed to stain with desmin and/or myogenin. Mean percentage of myogenin positive cells was significantly greater for ARMS (n = 31; mean percentage positivity 59% (95% confidence intervals ± 7%) than ERMS (n = 41, mean percentage positivity 16%, 95% confidence intervals ± 4; P < 0.0001). This data is consistent with previously published studies identifying strong nuclear myogenin staining in a high proportion of cells as a marker of alveolar histology.

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Acknowledgements

John Anderson’s work is supported by a Clinician Scientist Fellowship from Cancer Research UK.

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Authors and Affiliations

  1. Unit of Molecular Haematology and Cancer Biology, Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK

    D. A. Morgenstern, H. Rees & John Anderson

  2. Department of Histopathology, Great Ormond Street Hospital for Children, London, UK

    N. J. Sebire

  3. Department of Molecular Cytogenetics, Intsitute of Cancer Research, Sutton, Surrey, UK

    J. Shipley

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  1. D. A. Morgenstern
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  2. H. Rees
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  3. N. J. Sebire
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  4. J. Shipley
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  5. John Anderson
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Correspondence to John Anderson.

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Morgenstern, D.A., Rees, H., Sebire, N.J. et al. Rhabdomyosarcoma Subtyping by Immunohistochemical Assessment of Myogenin: Tissue Array Study and Review of the Literature. Pathol. Oncol. Res. 14, 233–238 (2008). https://doi.org/10.1007/s12253-008-9012-5

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  • DOI: https://doi.org/10.1007/s12253-008-9012-5

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