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Colorado Potato Beetle Resistance in Solanum oplocense X Solanum tuberosum Intercross Hybrids and Metabolite Markers for Selection

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Abstract

S. oplocense Hawkes, a wild relative of the potato S. tuberosum L. and source of resistance against the Colorado potato beetle Leptinotarsa decemlineata (Say) (CPB), was intercrossed with S. tuberosum. Backcross clones carried varying levels of resistance. Differences in foliar metabolites between resistant and susceptible clones were analyzed using liquid chromatography-mass spectrometry (LC-MS). Supervised machine learning classification methods uncorrelated shrunken centroids (USC), k-nearest neighbor (KNN) and support vector machines (SVM) were applied to develop algorithms that can classify resistant and susceptible plants using the metabolite data. Five metabolites were found to have a low error rate of prediction of CPB resistance. The five metabolites included two glycoalkaloids previously associated with resistance and susceptibility to CPB, dehydrocommersonine and solanine, respectively. Resistance was associated with a change in composition of glycoalkaloids to higher ratios of dehydrocommersonine over solanine.

Resumen

S. oplocense Hawkes, un pariente silvestre de la papa S. tuberosum L., y fuente de resistencia contra el escarabajo de Colorado Leptinotarsa decemlineata (Say) (CPB), se intercruzó con S. tuberosum. Los clones de la retrocruza conservaron diversos niveles de resistencia. Se analizaron las diferencias en los metabolitos foliares entre los clones resistentes y susceptibles usando espectrometría de cromatografía líquida de masas (LC-MS). Métodos supervisados de clasificación de aprendizaje de máquina no correlacionados con centroides encogidos (USC), k-cercanía de vecinos (KNN) y máquinas de respaldo de vector (SVM) se aplicaron para desarrollar algoritmos que pueden clasificar plantas resistentes y susceptibles usando los datos de los metabolitos. Se encontró que cinco metabolitos tenían un nivel bajo de error de predicción de la resistencia al CPB. Los cinco metabolitos incluyeron dos glicoalcaloides asociados previamente con resistencia y susceptibilidad al CPB, la deshidrocommersonina y la solanina, respectivamente. La resistencia se asoció con un cambio en la composición de los glicoalcaloides a altas proporciones de deshidrocommersonina sobre la solanina.

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Acknowledgments

The authors would like to thank Charlotte Davidson, Catherine Clark and Katherine Douglass who provided technical assistance. The work was funded by Agriculture and Agri-Food Canada Agricultural Bioproducts Innovation Program and the Developing Innovative Agri-products program.

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Authors and Affiliations

  1. Agriculture and Agri-Food Canada Potato Research Centre, P. O. Box 20280, 850 Lincoln Rd., Fredericton, N. B., Canada, E3B 4Z7

    Helen H. Tai, Kraig Worrall, David De Koeyer, Yvan Pelletier & George C. C. Tai

  2. Department of Chemistry, University of New Brunswick, Fredericton, N. B., Canada

    Kraig Worrall & Larry Calhoun

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  1. Helen H. Tai
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  2. Kraig Worrall
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  6. Larry Calhoun
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Correspondence to Helen H. Tai.

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Tai, H.H., Worrall, K., De Koeyer, D. et al. Colorado Potato Beetle Resistance in Solanum oplocense X Solanum tuberosum Intercross Hybrids and Metabolite Markers for Selection. Am. J. Potato Res. 92, 684–696 (2015). https://doi.org/10.1007/s12230-015-9484-2

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