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Combination of Accelerated Solvent Extraction and Micro-Solid-Phase Extraction for Determination of Trace Antibiotics in Food Samples

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Abstract

In this study, a novel sample preparation technique coupled with liquid chromatography was developed to determine the trace residues of antibiotics in food samples. Target analytes were extracted by accelerated solvent extraction (ASE), followed by the concentration and cleanup using micro-solid-phase extraction (μ-SPE). Variables affecting the ASE and μ-SPE were optimized to achieve the maximum extraction efficiency, besides the performance of the developed method was evaluated. Low detection limits of 7.4–16.3 ng/g and low quantification limits of 24.7–53.8 ng/g were achieved under optimized conditions. The recoveries of antibiotics ranged from 92 to 105 % with relative standard deviation of less than 9.3 %. The predominance was showed when compared to conventional ASE and μ-SPE. According to the results, ASE-μ-SPE was proved to be a simple and effective sample preparation method for the analysis of trace organic contaminants in food samples.

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Acknowledgment

This research was supported by National Natural Science Foundation of China (No. 21305013) and The Scientific Research Fund of Zhejiang Provincial Education Department (Y201328959) .

Conflict of Interest

Zhe Jiao declares that she has no conflict of interest. Dan Zhu declares that she has no conflict of interest. Weixuan Yao declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. College of Chemistry and Environmental Engineering, Dongguan University of Technology, Dongguan, 523808, China

    Zhe Jiao

  2. Henan Textile Products Supervision and Inspection Institute, Zhengzhou, 450007, China

    Dan Zhu

  3. Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou, 310053, China

    Weixuan Yao

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  1. Zhe Jiao
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  2. Dan Zhu
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  3. Weixuan Yao
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Correspondence to Zhe Jiao.

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Jiao, Z., Zhu, D. & Yao, W. Combination of Accelerated Solvent Extraction and Micro-Solid-Phase Extraction for Determination of Trace Antibiotics in Food Samples. Food Anal. Methods 8, 2163–2168 (2015). https://doi.org/10.1007/s12161-015-0105-y

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  • DOI: https://doi.org/10.1007/s12161-015-0105-y

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