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Rapid Screening of Oxytetracycline Residue in Fish Muscle by Dispersive Liquid-Liquid Microextraction and Europium-Sensitized Luminescence

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Abstract

A screening protocol was developed for oxytetracycline (OTC) residue in catfish muscle based on dispersive liquid-liquid microextraction (DLLME) and europium-sensitized luminescence (ESL). The analyte was extracted in HCl-ethylenediaminetetraacetic acid (EDTA)-acetonitrile, cleaned up by DLLME in water-acetonitrile-hexane-CH2Cl2, and detected by ESL using a portable luminescence photometer. A threshold was established at T = x 2 − 3σ2, where x 2 and σ2 were mean and standard deviation of ESL signals of catfish muscle samples (n = 20) spiked at 2 μg g−1, the tolerance set by the US Food and Drug Administration (FDA). Among 48 samples randomly spiked with OTC at 0–4 μg g−1, 46 were screened correctly without false negative, and 2 negatives were classified as presumptive positives. This reliable method cut usage of chlorinated solvent to 0.75 mL, reduced cost, and improved sample throughput.

Screening of OTC in catfish by DLLME-ESL.

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Compliance with Ethics Requirements

Guoxin Lai has received financial support from the Training Program of Fujian Excellent Talents in Universities, Fujian, China.

Conflict of Interest

Guoying Chen declares that he has no conflict of interest. Tuanwei Chen declares that he has no conflict of interest. Qiongqiong Li declares that she has no conflict of interest. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture (USDA). USDA is an equal opportunity employer. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Department of Chemical Engineering, Meizhouwan Vocational Technology College, Putian, Fujian, 351254, China

    Guoxin Lai

  2. U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA, 19038, USA

    Guoying Chen

  3. College of Food Science, Fujian University of Agriculture and Forestry, Fuzhou, Fujian, 350002, China

    Tuanwei Chen

  4. Shanghai Institute for Food and Drug Control, Shanghai, 201213, China

    Qiongqiong Li

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  1. Guoxin Lai
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  2. Guoying Chen
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  4. Qiongqiong Li
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Correspondence to Guoying Chen.

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Lai, G., Chen, G., Chen, T. et al. Rapid Screening of Oxytetracycline Residue in Fish Muscle by Dispersive Liquid-Liquid Microextraction and Europium-Sensitized Luminescence. Food Anal. Methods 8, 2052–2058 (2015). https://doi.org/10.1007/s12161-014-0076-4

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  • DOI: https://doi.org/10.1007/s12161-014-0076-4

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