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1H, 13C and 15N resonance assignments of the C-terminal domain of the 43 kDa subunit of the chloroplast signal recognition particle

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Abstract

We report the assignment of a 109 amino acid C-terminal chromo domain of the chloroplast signal recognition particle cpSRP43 subunit. cpSRP43 plays a crucial role in the targeting of light harvesting chlorophyll proteins to the thylakoids.

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References

  • Cavanagh J, Fairbrother WJ, Palmer AG III, Skelton NJ (1996) Protein NMR spectroscopy. Academic Press, San Diego

    Google Scholar 

  • Eichacker LA, Henry R (2001) Function of a chloroplast SRP in thylakoid protein export. Biochem Biophys Acta 1541:120–134

    Article  Google Scholar 

  • Funke S, Knechten T, Ollesch J, Schuenemann D (2005) A unique sequence motif in the 54-kDa subunit of the chloroplast signal recognition particle mediates binding to the 43-kDa subunit. J Biol Chem 280:8912–8917

    Article  Google Scholar 

  • Goddard TD, Kneller DG (1993) SPARKY 3. University of California, San Francisco

    Google Scholar 

  • Goforth RL, Peterson EC, Yuan J, Moore MJ, Kight AD, Lohse MB, Sakon J, Henry RL (2004) Regulation of the GTPase cycle in post-translational signal recognition particle-based protein targeting involves cpSRP43. J Biol Chem 279:43077–43084

    Article  Google Scholar 

  • Groves MR, Man A, Kahn A, Koch J, Dubel S, Robinson C, Sinning I (2001) Functional characterization of recombinant chloroplast signal recognition particle. J Biol Chem 276:27778–27786

    Article  Google Scholar 

  • Moore M, Goforth RL, Mori H, Henry R (2003) Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. J Cell Biol 162:1245–1254

    Article  Google Scholar 

  • Sivaraja V, Kumar TK, Leena PST, Chang An-ni, Vidya C, Goforth RL, Rajalingam D, Arvind K, Chou J, Henry RL, Yu C (2005) Three-dimensional solution structures of the chromo domains of cpSRP43. J Biol Chem 280:41465–41471

    Article  Google Scholar 

  • Tzvetkova-Chevolleau T, Hutin C, Noël LD, Goforth R, Carde JP, Caffarri S, Sinning I, Groves M, Teulon JM, Hoffman NE, Henry R, Havaux M, Nussaume L (2007) Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts. Plant Cell 19:1635–1648

    Article  Google Scholar 

  • Wishart DS, Sykes BD, Richards FM (1992) The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy. Biochemistry 31:1647–1651

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the National Institute for Health (NIH NCRR COBRE Grant 1 P20RR15569), the Department of Energy (DE-FGF02-01ER15161) and the Arkansas Biosciences Institute.

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Correspondence to Thallapuranam Krishnaswamy Suresh Kumar.

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Ananthamurthy, K., Kathir, K.M., Kight, A. et al. 1H, 13C and 15N resonance assignments of the C-terminal domain of the 43 kDa subunit of the chloroplast signal recognition particle. Biomol NMR Assign 2, 37–39 (2008). https://doi.org/10.1007/s12104-007-9078-0

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  • DOI: https://doi.org/10.1007/s12104-007-9078-0

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