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Serum miR-518e-5p is a potential biomarker for secondary imatinib-resistant gastrointestinal stromal tumor

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Abstract

Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumor of the intestinal tract. Imatinib is used as first-line therapy for GIST patients; however, secondary imatinib resistance poses a significant clinical challenge. Here, we analyzed serum miRNA expression profiles to identify specific serum miRNAs that could be used as early diagnostic markers. Candidate miRNAs were validated using Taqman quantitative PCR with serum samples from secondary imatinib-resistant GIST patients (n = 39), imatinib-sensitive GIST patients (n = 37), and healthy controls (n = 28). Serum miR-518e-5p and miR-548e levels were higher in secondary imatinib-resistant GIST than imatinib-sensitive GIST patients or healthy controls (P < 0.0001). However, ROC analysis indicated that only miR-518e-5p could distinguish imatinib-resistant GIST. To discriminate imatinib-resistant from imatinib-sensitive GIST patients, the AUC for serum miR-518e-5p was 0.9938, with 99.8% sensitivity and 82.1% specificity. Serum miR-518e-5p could also discriminate imatinib-resistant GIST patients from healthy controls with 99.9% sensitivity and 97.4% specificity. These data indicate that serum miR-518e-5p is a potentially promising non-invasive biomarker for early detection and diagnosis of secondary imatinib-resistant GIST.

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Acknowledgments

We would like to thank Dr. Weiwei Li from Beijing Microread Genetics Co., Ltd., for providing technical support for this study.

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Authors and Affiliations

  1. Department of Gastrointestinal and Nutriology Surgery, Affiliated Shengjing Hospital of China Medical University, Shenyang, 110004, China

    Youwei Kou, Ren Yang & Qiang Wang

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  1. Youwei Kou
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  2. Ren Yang
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  3. Qiang Wang
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Correspondence to Qiang Wang.

Additional information

Communicated by Sorab Dalal.

Corresponding editor: Sorab Dalal

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Kou, Y., Yang, R. & Wang, Q. Serum miR-518e-5p is a potential biomarker for secondary imatinib-resistant gastrointestinal stromal tumor. J Biosci 43, 1015–1023 (2018). https://doi.org/10.1007/s12038-018-9805-y

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  • DOI: https://doi.org/10.1007/s12038-018-9805-y

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