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Arabidopsis FLOWERING LOCUS D influences systemic-acquired-resistance-induced expression and histone modifications of WRKY genes

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Abstract

A plant that is in part infected by a pathogen is more resistant throughout its whole body to subsequent infections – a phenomenon known as systemic acquired resistance (SAR). Mobile signals are synthesized at the site of infection and distributed throughout the plant through vascular tissues. Mechanism of SAR development subsequent to reaching the mobile signal in the distal tissue is largely unknown. Recently we showed that FLOWERING LOCUS D (FLD) gene of Arabidopsis thaliana is required in the distal tissue to activate SAR. FLD codes for a homologue of human-lysine-specific histone demethylase. Here we show that FLD function is required for priming (SAR induced elevated expression during challenge inoculation) of WRKY29 and WRKY6 genes. FLD also differentially influences basal and SAR-induced expression of WRKY38, WRKY65 and WRKY53 genes. In addition, we also show that FLD partly localizes in nucleus and influences histone modifications at the promoters of WRKY29 and WRKY6 genes. The results altogether indicate to the possibility of FLD’s involvement in epigenetic regulation of SAR.

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Acknowledgements

This work is supported by Department of Biotechnology (DBT) grant (BT/PR14656/BRB/10/864/2010) to AKN, University Grant Commission merit fellowship to DS and Council for Scientific and Industrial Research fellowship to SR. We acknowledge Jyoti Shah for comments and help in manuscript preparation.

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Authors and Affiliations

  1. School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India

    Vijayata Singh, Shweta Roy, Deepjyoti Singh & Ashis Kumar Nandi

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  1. Vijayata Singh
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  2. Shweta Roy
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  3. Deepjyoti Singh
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  4. Ashis Kumar Nandi
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Correspondence to Ashis Kumar Nandi.

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Corresponding editor: Utpal Nath

[Singh V, Roy S, Singh D and Nandi AK 2014 Arabidopsis FLOWERING LOCUS D influences systemic-acquired-resistance-induced expression and histone modifications of WRKY genes. J. Biosci. 39 1–8] DOI 10.1007/s12038-013-9407-7

Supplementary materials pertaining to this article are available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/mar2014/supp/Singh.pdf

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Singh, V., Roy, S., Singh, D. et al. Arabidopsis FLOWERING LOCUS D influences systemic-acquired-resistance-induced expression and histone modifications of WRKY genes. J Biosci 39, 119–126 (2014). https://doi.org/10.1007/s12038-013-9407-7

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