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Efficient Non-sterilized Fermentation of Biomass-Derived Xylose to Lactic Acid by a Thermotolerant Bacillus coagulans NL01

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Abstract

Xylose is the major pentose and the second most abundant sugar in lignocellulosic feedstock. Its efficient utilization is regarded as a technical barrier to the commercial production of bulk chemicals from lignocellulosic biomass. This work aimed at evaluating the lactic acid production from the biomass-derived xylose using non-sterilized fermentation by Bacillus coagulans NL01. A maximum lactic acid concentration of about 75 g/L was achieved from xylose of 100 g/L after 72 h batch fermentation. Acetic acid and levulinic acid were identified as important inhibitors in xylose fermentation, which markedly reduced lactic acid productivity at 15 and 1.0 g/L, respectively. But low concentrations of formic acid (<2 g/L) exerted a stimulating effect on the lactic acid production. When prehydrolysate containing total 25.45 g/L monosaccharide was fermented with B. coagulans NL01, the same preference for glucose, xylose, and arabinose was observed and18.2 g/L lactic acid was obtained after 48 h fermentation. These results proved that B. coagulans NL01 was potentially well-suited for producing lactic acid from underutilized xylose-rich prehydrolysates.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31070513) and Excellent Youth Foundation of Jiangsu Province of China (BK2012038). The authors are also grateful to the Major Program of the Natural Science Foundation of Jiangsu Higher Education of China (10KJA22019) and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) for partial funding of this study.

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Authors and Affiliations

  1. College of Chemical Engineering, Nanjing Forestry University, No. 159 Long Pan Road, Nanjing, 210037, China

    Jia Ouyang, Cong Cai, Hai Chen, Ting Jiang & Zhaojuan Zheng

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  1. Jia Ouyang
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  2. Cong Cai
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  3. Hai Chen
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  4. Ting Jiang
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  5. Zhaojuan Zheng
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Correspondence to Jia Ouyang.

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Ouyang, J., Cai, C., Chen, H. et al. Efficient Non-sterilized Fermentation of Biomass-Derived Xylose to Lactic Acid by a Thermotolerant Bacillus coagulans NL01. Appl Biochem Biotechnol 168, 2387–2397 (2012). https://doi.org/10.1007/s12010-012-9944-9

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  • DOI: https://doi.org/10.1007/s12010-012-9944-9

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