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Gaseous NO2 effects on stomatal behavior, photosynthesis and respiration of hybrid poplar leaves

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Abstract

In this study, we used poplar as a model plant and investigated the effects of gaseous nitrogen dioxide (NO2, 4 µl 1−1) on stomatal conductance, photosynthesis, dark- and photorespiration of Populus alba × Populus berolinensis hybrid leaves using the photosynthesis system and scanning electron microscope technique. The results showed that net photosynthetic rates were significantly reduced in leaves exposed to 4 µl 1−1 NO2 for 48 h as compared with leaves exposed to ambient carbon dioxide 380 µl 1−1 and ambient NO2 <0.1 µl 1−1 (the control) and the leaves exposed for 14 h. The decline of net photosynthetic rate was caused mainly by NO2 treatment. Dark respiration rates were dependent on co-action of the two factors (leaf temperature and NO2 treatment time). Post-illumination carbon dioxide burst in the exposed leaves occurred at 13–15 s after turning the light off, whereas this phenomenon was absent in the control leaves.

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Acknowledgments

This work was supported by grants from the “Fundamental Research Funds for the Central Universities” (Grant No. 2572014CA24; DL10BB24), ‘National Natural Science Foundation’ (Grant No. 31300506), and The Major project for the Heilongjiang Province Science and Technology Program (GZ13B004). The authors gratefully acknowledged Mr. Thomas D. Dahmer and Dr. Josirley de FC Carvalho for language correction.

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Authors and Affiliations

  1. College of Life Science, Northeast Forestry University, 26# Hexing Road, P. O. Box 123, Harbin, 150040, People’s Republic of China

    Yanbo Hu, Jian Diao, Ke Wang, Rui Yang & Guangyu Sun

  2. USDA-ARS, Crop Genetics Research Unit, Stoneville, USA

    Nacer Bellaloui

  3. Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O.Box 49, SE-230 50, Alnarp, Sweden

    Mulualem Tigabu

  4. College of Landscape, Northeast Forestry University, Harbin, 150040, People’s Republic of China

    Jinghong Wang

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  1. Yanbo Hu
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  2. Nacer Bellaloui
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  6. Ke Wang
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  8. Guangyu Sun
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Correspondence to Yanbo Hu.

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Communicated by Z. Miszalski.

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Hu, Y., Bellaloui, N., Tigabu, M. et al. Gaseous NO2 effects on stomatal behavior, photosynthesis and respiration of hybrid poplar leaves. Acta Physiol Plant 37, 39 (2015). https://doi.org/10.1007/s11738-014-1749-8

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  • DOI: https://doi.org/10.1007/s11738-014-1749-8

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