Skip to main content
Log in

mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP

  • Research Article
  • Published:
Frontiers of Agriculture in China

Abstract

The polymorphism of mitochondrial DNA (mtD-NA) D-loop of 83 individuals from nine Chinese indigenous sheep breeds and two imported sheep breeds was studied with five endonucleases, Hinf I, Msp I, Sau3 A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The average polymorphic degree (π value = 0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very low.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • An Z Y, Li Y L (1995). Investigation and verification of Hu sheep history. Chinese Agricultural History, 14(1): 15–18 (in Chinese)

    Google Scholar 

  • Cao D G, Du L X, Li S Q (2002). RAPD analysis of 4 sheep breeds. Chinese Journal of Animal Science, 38(2): 12–14 (in Chinese)

    Google Scholar 

  • Compiling Group of Sheep and Goat Breeds in China (1989). Sheep and Goat Breeds in China. Shanghai: Shanghai Scientific & Technical Publishers, 3–4 (in Chinese)

    Google Scholar 

  • Gong Y F, Li X L, Liu Z Z, Li J Q (2002). Study on RAPD of Chinese main indigenous sheep breeds. Hereditas (Beijing), 24(4): 423–426 (in Chinese)

    Google Scholar 

  • Hiendleder S, Mainz K, Plante Y, Lewalski H (1998). Analysis of mitochondrial DNA indicates that domestic sheep are derived from two different ancestral maternal sources: No evidence for contributions from Urial and Argali sheep. Hered, 89(2): 113–120

    Article  CAS  Google Scholar 

  • Hiendleder S, Phua S H, Hecht W (1999). A diagnostic assay discriminating between two major Ovis aries mitochondrial DNA haplogroups. Anim Genet, 30(30): 211–213

    Article  PubMed  CAS  Google Scholar 

  • Lan R, Hong Q H, Gao Y H, Zhang J, Su B, Wang W, Liu A H, Zhang Y P, Shi L M (1998). Study on genetic polymorphism of mtDNA of Yunnan sheep. Hereditas (Beijing), 20(1): 20–23 (in Chinese)

    CAS  Google Scholar 

  • Li Q (1997). Re-discussion on origin and history of Hu sheep. Chinese Agricultural History, 16(2): 91–95 (in Chinese)

    Google Scholar 

  • Li X L, Gong Y F, Zhang J W, Liu Z Z, Allessio V (2004a). Study on polymorphisms of microsatellites DNA of six Chinese indigenous sheep breeds. Acta Genetica Sinica, 31(11): 1203–1210 (in Chinese)

    PubMed  CAS  Google Scholar 

  • Li X L, Zhang Z L, Gong Y F, Liu Z Z, Wang X J (2004b). Random amplified polymorphic DNA of Chinese indigenous sheep breeds. Chinese Journal of Veterinary Science, 24(5): 508–510 (in Chinese)

    Article  CAS  Google Scholar 

  • Li X L, Liu Z Z, Tian Q Y (1998). Study on RFLP of mtDNA of Ujumqin sheep. Journal of Hebei Agrotechnical Teachers College, 12(3): 1–4 (in Chinese)

    CAS  Google Scholar 

  • Li X L, Ma G X, Tian Q Y, Liu Z Z (2000). Study on RFLP of mtDNA of Mongolia sheep. Journal of Inner Mongolia Agricultural University, 21(3): 50–54 (in Chinese)

    CAS  Google Scholar 

  • Li X L, Tian Q Y, Liu Z Z, Sun N Q, Ma G X (2001). Study on RFLP of mtDNA of several indigenous sheep breeds in China. Acta Veterinaria et Zootechnica Sinica, 32(4): 295–298 (in Chinese)

    Google Scholar 

  • Liu Z L, Wei H, Zeng Y Z, Wang A D, Gan S X (2001). Analysis of the polymorphism of mtDNA D-loop in three breeds of laboratory miniature pig in China. Hereditas (Beijing), 23(2): 123–127 (in Chinese)

    CAS  Google Scholar 

  • Nei M, Li W H (1979). Mathematic model for studying genetic variation in terms of restriction endonucleases. PNAS USA, 76: 5269–5273

    Article  PubMed  CAS  Google Scholar 

  • Tu Z C, Zhang Y P (1998). Study on genetic diversity of mtDNA of Tibetan sheep. Acta Veterinaria et Zootechnica Sinica, 29(2): 132–135 (in Chinese)

    Google Scholar 

  • Xie C X (1985). History of Chinese Cattle, Sheep and Goat Feeding (appended by simple history of deer feeding). Beijing: China Agricultural Publishing House, 140 (in Chinese)

    Google Scholar 

  • Zhao X B, Chu M X, Li N, Gong G C, Wu C X (2001). PCR-SSCP and sequencing analysis on 5′ terminal region of mtDNA control region in sheep. Acta Genetica Sinica, 28(3): 225–228 (in Chinese)

    PubMed  CAS  Google Scholar 

  • Zhu X H (1986). Collection of Historical Data on Chinese Animal Husbandry. Beijing: Beijing Science & Technology Press, 160–167 (in Chinese)

    Google Scholar 

  • Zou J Z (1994). The Ancient History of Animal Farming and Veterinary in China. Beijing: China Agricultural Science & Technology Press, 101 (in Chinese)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Li Xianglong.

Additional information

Translated from Hereditas, 2006, 28(2): 165–170 [译自: 遗传]

About this article

Cite this article

Li, X., Zhang, Z., Gong, Y. et al. mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP. Front. Agric. China 1, 352–356 (2007). https://doi.org/10.1007/s11703-007-0059-y

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11703-007-0059-y

Keywords

Navigation