Summary
To re-identify and further group 25 isolates of Trichosporon spp. identified morphologically previously, sequences of D1/D2 region of large subunit (LSU) of ribosomal DNA (rDNA) of 25 tested strains for identification and those of ribosomal intergenic space 1 (IGS1) region of 11 strains for subgrouping were detected. The identifications of tested strains were changed except 6 strains. According to the alignment of the IGS1 region, 6 T. asahii isolates tested fell into 4 groups and 5 T. faecale isolates into 3 groups. Polymorphism of 2 T. japonicum isolates was found in 10 positions. With the alignments obtained in this research compared with the relative GenBank entries, it was found that T. asahii, T. faecale and T. japonicum species were divided into 7, 3 and 2 subtypes respectively. Morphological and biophysical methods are not sufficient for Trichosporon spp. identification. Sequencing becomes necessary for Trichosporon diagnosis. There is obvious diversity within a species.
Similar content being viewed by others
References
Hoy J, Hsu KC, Rolston, et al. Trichosporon beigelii infection: a review. Rev Infect Dis, 1986,8(6):959–967
Marty FM, Barouch DH, Coakley EP, et al. Disseminated trichosporonosis caused by Trichosporon loubieri. J Clin Microbiol, 2003,41(12):5317–5320
Nahass GT, Rosenberg SP, Leonardi, CL, et al. Disseminated infection with Trichosporon beigelii. Report of a case and review of the cutaneous and histologic manifestations. Arch Dermatol, 1993,129(8):1020–1023
Watson KC, Kallichurum S. Brain abscess due to Trichosporon cutaneum. J Med Microbiol, 1970,3(1):191–193
Yang R, Ao J, Wang W, et al. Disseminated trichosporonosis in China. Mycoses, 2003, 46(11–12):519–523
Fell JW, Boekhout T, Fonseca A, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol, 2000,50(3):1351–1371
Rodriguez-Tudela JL, Diaz-Guerra TM, Mellado E, et al. Susceptibility patterns and molecular identification of Trichosporon species. Antimicrob Agents Chemother, 2005, 49(10):4026–4034
Sugita T, Nishikawa A, Ikeda R, et al. Identification of medically relevant Trichosporon species based on sequences of internal transcribed spacer regions and construction of a database for Trichosporon identification. J Clin Microbiol, 1999,37(6):1985–1993
Sugita T, Ikeda R, Nishikawa A. Analysis of Trichosporon isolates obtained from the houses of patients with summer-type hypersensitivity pneumonitis. J Clin Microbiol, 2004,42(12): 5467–5471
Fell JW, Scorzetti G. Reassignment of the basidiomycetous yeasts Trichosporon pullulans to Guehomyces pullulans gen. nov., comb. nov. and Hyalodendron lignicola to Trichosporon lignicola comb. nov. Int J Syst Evol Microbiol, 2004,54(3):995–998
Middelhoven WJ. Trichosporon wieringae sp. nov., an anamorphic basidiomycetous yeast from soil, and assimilation of some phenolic compounds, polysaccharides and other non-conventional carbon sources by saprophytic Trichosporon species. Antonie Van Leeuwenhoek, 2004, 86(4):329–337
Middelhoven WJ, Scorzetti G, Fell JW. Systematics of the anamorphic basidiomycetous yeast genus Trichosporon Behrend with the description of five novel species: Trichosporon vadense, T. smithiae, T. dehoogii, T. Scarabaeor-um and T. gamsii. Int J Syst Evol Microbiol, 2004,54(3):975–986
Middelhoven WJ, Scorzetti G, Fell JW. Trichosporon veenhuisii sp. nov., an alkane-assimilating anamorphic basidiomycetous yeast. Int J Syst Evol Microbiol, 2000, 50(1):381–387
Molnar O, Schatzmayr G, Fuchs E, et al. Trichosporon mycotoxinivorans sp. nov., a new yeast species useful in biological detoxification of various mycotoxins. Syst Appl Microbiol, 2004,27(6):661–671
Sugita T, Nakase T. Trichosporon japonicum sp. nov. isolated from the air. Int J Syst Bacteriol, 1998,48(4):1425–1429
Sugita T, Takashima M, Nakase T, et al. Two new yeasts, Trichosporon debeurmannianum sp. nov. and Trichosporon dermatis sp. nov., transferred from the Cryptococcus humicola complex. Int J Syst Evol Microbiol, 2001,51(3): 1221–1228
Sugita T, Nakajima M, Ikeda R, et al. Sequence analysis of the ribosomal DNA intergenic spacer 1 regions of Trichosporon species. J Clin Microbiol, 2002,40(5):1826–1830
Abliz P, Fukushima K, Takizawa K,et al. Identification of the first isolate of Trichosporon asahii var asahii from disseminated trichospornosis in China. Diagn Microbiol Infect Dis, 2002,44(1): 17–22
Chang SE, Kim KJ, Lee WS, et al. A case of Trichosporon cutaneum folliculitis and septicaemia. Clin Exp Dermatol, 2003,28(1):37–38
Gueho E, Improvisi L, de Hoog GS, et al. Trichosporon on humans: a practical account. Mycoses, 1994,37(1–2):3–10
Nettles RE, Nichols LS, Bell-McGuinn K, et al. Successful treatment of Trichosporon mucoides infection with fluconazole in a heart and kidney transplant recipient. Clin Infect Dis, 2003,36(4):E63–E66
Sugita T, Nishikawa A, Shinoda T, et al. Taxonomic position of deep-seated, mucosa-associated, and superficial isolates of Trichosporon cutaneum from trichosporonosis patients. J Clin Microbiol, 1995,33(5): 1368–1370
Author information
Authors and Affiliations
Corresponding author
Additional information
This project was partly supported by the National Bio-Resource Project “Pathogenic microbes” from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Governments.
Rights and permissions
About this article
Cite this article
Zeng, J., de Souza Motta, C.M., Fukushima, K. et al. Identification of Trichosporon spp. strains by sequencing D1/D2 region and sub-typing by sequencing ribosomal intergenic spacer region of ribosomal DNA. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 655–658 (2009). https://doi.org/10.1007/s11596-009-0524-3
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11596-009-0524-3