Abstract
Utilization as dry mycelial fertilizer (DMF) produced from penicillin fermentation fungi mycelium (PFFM) with an acid-heating pretreatment is a potential way. To study the transformation and stability of water-extractable organic matter in DMF-amended soil via fluorescence regional integration (FRI) of fluorescence excitation-emission matrix (EEM), a soil experiment in pot was carried out. The results showed that residual penicillin (about 32 mg/kg) was almost degraded in the first 5 days, indicating that the drug pollution was in control. The pH value, DOC, DON, and DOC/DON presented a classical profile, but germination index (GI) leveled off about 0.13 till day 13 in DMF-12% treatment due to the severe phytotoxicity. The addition of DMF significantly increased the soil microbial populations in contrast to the CON treatment. The EEM showed that the protein-like and microbial byproduct-like matters vanished on the 25th and 33rd days, whereas the fulvic-like substances appeared on the 7th day. The humic-like substances existed in original samples but their content greatly enhanced finally. The FRI results showed that P V, n/P III, n reached the highest value of 1.84 on the 25th day, suggesting that DMF maintained stable in amended soil. Because of its consistency with the results of GI and DOC/DON, the EEM-FRI has a potential to evaluate the stability of DMF in soil.
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References
Antízar-Ladislao B, Lopez-Real J, Beck AJ (2006) Investigation of organic matter dynamics during in-vessel composting of an aged coaltar contaminated soil using fluorescence excitation-emission spectroscopy. Chemosphere 64:839–847
Bahram M, Bro R, Stedmon C, Afkhami A (2010) Handling of Rayleigh and Raman scatter for PARAFAC modeling of fluorescence data using interpolation. J Chemom 20:99–105
Baker DA, Inverarity R (2004) Protein-like fluorescence intensity as a possible tool for determining river water quality. Hydrol Process 18:2927–2945
Bernal MP, Alburquerque JA, Moral R (2009) Composting of animal manures and chemical criteria for compost maturity assessment. A review. Bioresour Technol 100:5444–5453
Chen S, Dong H, Fan Y, Li W, Cohen Y (2006) Dry mycelium of Penicillium chrysogenum induces expression of pathogenesis-related protein genes and resistance against wilt diseases in Bt transgenic cotton. Biol Control 39:460–464
Chen W, Westerhoff P, Leenheer JA, Booksh K (2003) Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environ Sci Technol 37:5701–5710
Chen Z, Zhang S, Wen Q, Zheng J (2015) Effect of aeration rate on composting of penicillin mycelial dreg. J Environ Sci 37:172–178
Coble PG (1996) Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy. Mar Chem
Gao M, Li B, Yu A, Liang F, Yang L, Sun Y (2010) The effect of aeration rate on forced-aeration composting of chicken manure and sawdust. Bioresour Technol 101:1899–1903
He X, Xi B, Wei Z, Jiang Y, Geng C, Yang Y, Yuan Y, Liu H (2011a) Physicochemical and spectroscopic characteristics of dissolved organic matter extracted from municipal solid waste (MSW) and their influence on the landfill biological stability. Bioresour Technol 102:2322–2327
He X, Xi B, Wei Z, Guo X, Li M, An D, Liu H (2011b) Spectroscopic characterization of water extractable organic matter during composting of municipal solid waste. Chemosphere 82:541–548
He X, Xi B, Jiang Y, He L, Li D, Pan H, Bai S (2013) Structural transformation study of water-extractable organic matter during the industrial composting of cattle manure. Microchem J 106:160–166
Ko H, Kim K, Kim H, Kim C, Umeda M (2008) Evaluation of maturity parameters and heavy metal contents in composts made from animal manure. Waste Manag 28:813–820
Lv B, Xing M, Yang J, Qi W, Lu Y (2013) Chemical and spectroscopic characterization of water extractable organic matter during vermicomposting of cattle dung. Bioresour Technol 132:320–326
Lv B, Xing M, Zhao C, Yang J, Xiang L (2014) Towards understanding the stabilization process in vermicomposting using PARAFAC analysis of fluorescence spectra. Chemosphere 117:216–222
Marschner P, Kandeler E, Marschner B (2003) Structure and function of the soil microbial community in a long-term fertilizer experiment. Soil Biol Biochem 35:453–461
Pascual JA, García C, Hernandez T (1999) Comparison of fresh and composted organic waste in their efficacy for the improvement of arid soil quality. Bioresour Technol 68:255–264
Picón-Rubio FJ, Kawas JR, Fimbres-Durazo H, Ibarra-Gil H, Garza-Cazares F, Ledezma-Torres R, Andrade-Montemayor H (2010) Effect of substituting soybean meal with mycelium of Penicillium chrysogenum in lamb diets on performance and carcass quality. Small Rumin Res 91:127–131
Raj D, Antil RS (2012) Evaluation of maturity and stability parameters of composts prepared from agro-industrial wastes. Arch Agron Soil Sci 58:817–832
Said-pullicino D, Erriquens FG, Gigliotti G (2007) Changes in the chemical characteristics of water-extractable organic matter during composting and their influence on compost stability and maturity. Bioresour Technol 98:1822–1831
Sellami F, Hachicha S, Chtourou M, Medhioub K, Ammar E (2008a) Maturity assessment of composted olive mill wastes using UV spectra and humification parameters. Bioresour Technol 99:6900–6907
Sellami F, Hachicha S, Chtourou M, Medhioub K, Ammar E (2008b) Maturity assessment of composted olive mill wastes using UV spectra and humification parameters. Bioresour Technol 99:6900–6907
Shao Z, He P, Zhang D, Shao L (2009) Characterization of water-extractable organic matter during the biostabilization of municipal solid waste. J Hazard Mater 164:1191–1197
Stedmon CA, Markager S, Bro R (2003) Tracing dissolved organic matter in aquatic environments using a new approach to fluorescence spectroscopy. Mar Chem 82:239–254
Suthar S, Sharma P (2013) Vermicomposting of toxic weed—Lantana camara biomass: chemical and microbial properties changes and assessment of toxicity of end product using seed bioassay. Ecotoxicol Environ Saf 95:179–187
Tian W, Li L, Liu F, Zhang Z, Yu G, Shen Q, Shen B (2012) Assessment of the maturity and biological parameters of compost produced from dairy manure and rice chaff by excitation-emission matrix fluorescence spectroscopy. Bioresour Technol 110:330–337
Wang B, Liu H, Cai C, Thabit M, Wang P, Li G, Duan Z (2016) Effect of dry mycelium of Penicillium chrysogenum fertilizer on soil microbial community composition, enzyme activities and snap bean growth. Environ Sci Pollut Res 23:20728–20738
Wang K, Li W, Gong X, Li Y, Wu C, Ren N (2013) Spectral study of dissolved organic matter in biosolid during the composting process using inorganic bulking agent: UV–vis, GPC, FTIR and EEM. Int Biodeterior Biodegrad 85:617–623
Wu C, Li Y, Li W, Wang K (2015) Characterizing the distribution of organic matter during composting of sewage sludge using a chemical and spectroscopic approach. RSC Adv 5:95960–95966
Wu D, Lü F, Gao H, Shao L, He P (2011) Mesophilic bio-liquefaction of lincomycin manufacturing biowaste: the influence of total solid content and inoculum to substrate ratio. Bioresour Technol 102:5855–5862
Wu H, Zhou Z, Zhang Y, Chen T, Wang H, Lu W (2012) Fluorescence-based rapid assessment of the biological stability of landfilled municipal solid waste. Bioresour Technol 110:174–183
Xing M, Li X, Yang J, Huang Z, Lu Y (2012) Changes in the chemical characteristics of water-extracted organic matter from vermicomposting of sewage sludge and cow dung. J Hazard Mater 205-206:24–31
Yang L, Zhang S, Chen Z, Wen Q, Wang Y (2016) Maturity and security assessment of pilot-scale aerobic co-composting of penicillin fermentation dregs (PFDs) with sewage sludge. Bioresour Technol 204:185–191
Zhang G, Li C, Ma D, Zhang Z, Xu G (2015b) Anaerobic digestion of antibiotic residue in combination with hydrothermal pretreatment for biogas. Bioresour Technol 192:257–265
Zhang S, Chen Z, Wen Q, Ma J, He Z (2016b) Assessment of maturity during co-composting of penicillin mycelial dreg via fluorescence excitation-emission matrix spectra: characteristics of chemical and fluorescent parameters of water-extractable organic matter. Chemosphere 155:358–366
Zhang S, Chen Z, Wen Q, Yang L, Wang W, Zheng J (2016a) Effectiveness of bulking agents for co-composting penicillin mycelial dreg (PMD) and sewage sludge in pilot-scale system. Environ Sci Pollut Res 23:1362–1370
Zhang S, Chen Z, Wen Q, Zheng J (2016c) Assessing the stability in composting of penicillin mycelial dreg via parallel factor (PARAFAC) analysis of fluorescence excitation–emission matrix (EEM). Chem Eng J 299:167–176
Zhang Z, Zhao J, Yu C, Dong S, Zhang D, Yu R, Wang C, Liu Y (2015a) Evaluation of aerobic co-composting of penicillin fermentation fungi residue with pig manure on penicillin degradation, microbial population dynamics and composting maturity. Bioresour Technol 198:403–409
Zmora-Nahum S, Markovitch O, Tarchitzky J, Chen Y (2005) Dissolved organic carbon (DOC) as a parameter of compost maturity. Soil Biol Biochem 37:2109–2116
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This work was financially supported by HIT Environment and Ecology Innovation Special Funds (No. HSCJ201613), National Natural Science Foundation of China (51678189).
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Wang, B., Cai, C., Li, G. et al. Assessing the stability in dry mycelial fertilizer of Penicillium chrysogenum as soil amendment via fluorescence excitation-emission matrix spectra: organic matter’s transformation and maturity. Environ Sci Pollut Res 24, 28258–28267 (2017). https://doi.org/10.1007/s11356-017-0086-z
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DOI: https://doi.org/10.1007/s11356-017-0086-z