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Agrobacterium-Mediated Transformation of Common Bermudagrass (Cynodon dactylon)

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Abstract

Common bermudagrass, Cynodon dactylon, is a widely used warm-season turf and forage species in the temperate and tropical regions of the world. We have been able to transform the species using Agrobacterium-mediated approach. In seven experiments reported here, a total of 67 plates of calluses and suspensions were infected with Agrobacterium tumefaciens strains, and nine hygromycin B resistant calluses were obtained after selection. Among them two green independent transgenic plants were recovered. The plants growing in pots looked relatively compact at the beginning, but the ploidy level of the plants, as determined by nuclear DNA content, was not altered.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

ABA:

abscisic acid

BAP:

6-benzylaminopurine

GA:

gibberellic acid

Hph:

hygromycin phosphate transferase

hyg B:

hygromycin B

NAA:

α-naphthaleneacetic acid

SEC:

somatic embryo cluster

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Authors and Affiliations

  1. Department of Crop Science, North Carolina State University, Raleigh, NC, 27695-7620, USA

    L. Li, R. Li & R. Qu

  2. Department of Horticulture, Iowa State University, Ames, IA, 50011, USA

    S. Fei

Authors
  1. L. Li
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  2. R. Li
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  3. S. Fei
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  4. R. Qu
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Correspondence to R. Qu.

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Li, L., Li, R., Fei, S. et al. Agrobacterium-Mediated Transformation of Common Bermudagrass (Cynodon dactylon). Plant Cell Tiss Organ Cult 83, 223–229 (2005). https://doi.org/10.1007/s11240-005-6838-3

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  • DOI: https://doi.org/10.1007/s11240-005-6838-3

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