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Molecular Cloning, Sequence Analysis, and Expression of the Polygalacturonase-inhibiting Protein (PGIP) Gene in Mulberry

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Abstract

A full-length cDNA sequence encoding polygalacturonase-inhibiting protein (PGIP) from mulberry, which we designated MPGIP (GenBank accession no.: HM044383), was cloned based on mulberry expressed sequence tags (ESTs). Sequence analysis showed that the MPGIP is 1,274 base pairs (bp) in length, encoding 333 amino acids with a predicted molecular weight of 37.29 kDa and an isoelectric point of 7.25. The expression levels of MPGIP at different developmental stages in mulberry leaves and flowers and in different tissues were investigated. The results showed that MPGIP transcripts were most abundantly expressed in the young leaf, and the expression level of the mRNA could be increased significantly under salicylic acid (SA), abscissic acid (ABA), and NaCl stress conditions compared to the normal growth environment. And we also expressed the recombinant protein. This is to help understand a molecular basis for signal transduction mechanism underlying the stress response in mulberry.

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References

  • Abu-Goukh AA, Strand LL, Labavitch JM (1983) Development related changes in decay susceptibility and PG inhibitor content of ‘Bartlett’ pear fruit. Physiol Plant Pathol 23:101–109

    Article  CAS  Google Scholar 

  • Albersheim P, Anderson AJ (1971) Protein from plant cell wall inhibit polygalacturonases secreted by plant pathogens. PNAS USA 68:1815–1819

    Article  PubMed  CAS  Google Scholar 

  • Bergmann CW, Ito Y, Singer D et al (1994) Polygalacturonase-inhibiting protein accumulates in Phaseolus vulgaris L. in response to wounding, elicitors and fungal infection. Plant J 5:625–634

    Article  PubMed  CAS  Google Scholar 

  • De Lorenzo G, D’Ovidio R, Cervone F (2001) The role of polygalacturonce inhibiting proteins (PGIPs) in defense against pathogenic fungi. Annu Rev Phytopathol 39:313–315

    Article  PubMed  Google Scholar 

  • Di Matteo A, Federici L, MaRei B, Salvi G, Johnson KA, Savino C, De Lorenzo G, Tsernoglou D, Cervone F (2003) The crystal structure of polygalacturonase inhibiting protein (PGIP), a leucine rich repeat protein involved in plant defense. PNAS USA 100:10124–10128

    Article  PubMed  Google Scholar 

  • Di Matteo A, Bonivento D, Tsemoglou D, Fedefici L, Cervone F (2006) Polygalacturonase-inhibiting protein (PGIP) in plant defense: a structural view. Phytochemistry 67:528–533

    Article  PubMed  Google Scholar 

  • D’Ovidio R, Mattei B, Roberti S, Bellincampi D (2004) Polygalacturonases, polygalacturonase inhibiting proteins and pectic oligomers in plant–pathogen interactions. Biochim Biophys Acta 1696:237–244

    PubMed  Google Scholar 

  • Fang RJ, Qi JL, Hu DQ et al (2008) Construction of cDNA expression library from the young leaves of mulberry and EST Analysis. Sericultural Sci 34:581–586

    CAS  Google Scholar 

  • Gao Y, Zhao Y (2010) Cloning and characterization of a G protein β Subunit gene responsive to plant hormones and abiotic stresses in Brassica napus. Plant Mol Biol Rep 28:450–459

    Article  CAS  Google Scholar 

  • Gazendama I, Oelofse D, Berger DK (2004) High-level expression of apple PGIP1 is not sufficient to protect transgenic potato against Verticillium dahliae. Physiol Plant Pathol 65(3):145–155

    Article  Google Scholar 

  • Gotoh Y, Nalumpang S, Isshiki A et al (2002) A cDNA encoding polygalacturonase inhibiting protein induced in citrus leaves by polygalacturonase of Alternaria citri. J Gen Plant Pathol 68:57–61

    Article  CAS  Google Scholar 

  • Guo S, Tian RX et al (2011) Cloning and expression analysis of the smooth-edge seed gene GSE-1 from Cucurbita pepo L. Plant Mol Biol Rep 29:498–504

    Article  CAS  Google Scholar 

  • Haas BJ, Volfvsky N, Town CD et al (2002) Full-length messenger RNA sequences greatly improve genome annotation. Genome Biol 3: Research 0029.01–0029.12

  • Johhnston DJ, Rananthan V, Williamson B (1993) A protein from immature raspberry fruits which inhibits endopolygalacturonases from Botrytis cinerea and other micro-organisms. J Exp Bot 44:971–976

    Article  Google Scholar 

  • Lang C, Dornenburg H (2000) Perspectives in the biological function and the technological of polygalacturonases. Appl Microbiol Biotechnol 53:366–375

    Article  PubMed  CAS  Google Scholar 

  • Leckie F, Mattei B, Capodicasa C, Hemmings A, Nuss L, Aracri B, De Lorenzo G, Cervone F (1999) The specificity of polygalacturonase-inhibiting protein (PGIP): a single amino acid substitution in the solvent-exposed p-strand/13·turn region of the leucine-rich repeats (LRRs) confers a new recognition capability. EMBO J 18:2352–2363

    Article  PubMed  CAS  Google Scholar 

  • Liang F, Zhang K, Zhou C et al (2005) Cloning, characterization and expression of the gene encoding polygalacturonase-inhibiting proteins (PGIPs) of peach [Prunus persica (L.) Batch]. Plant Sci 168:481–486

    Article  CAS  Google Scholar 

  • Nalumpang S, Gotoh Y, Tsuboi H et al (2002a) Functional characterization of citrus polygalacturonase-inhibiting protein. J Gen Plant Pathol 68:118–127

    Article  CAS  Google Scholar 

  • Nalumpang S, Gotoh Y et al (2002b) Comparison and characterization of zation polygalacturonase inhibiting protein genes from the genus of Citrus and its close related genera. Thai J Agric Sci 35:112–117

    Google Scholar 

  • Pan G, Lou C (2008) Isolation of an 1-aminocyclopropane-1-carboxylate oxidase gene from mulberry (Morus alba L.) and analysis of the function of this gene in plant development and stresses response. J Plant Physiol 165:1204–1213

    Article  PubMed  CAS  Google Scholar 

  • Rai MK, Asthana P, Singh SK, Jaiswal VS, Jaiswal U (2009) The encapsulation technology in fruit plants: a review. Biotechnol Adv 27:671–679

    Article  PubMed  Google Scholar 

  • Ruan QP, Zhou L, Zheng Y (2000) Progresses of research on PGIP of resisting plant diseases. Chin Bull Bot 17:60–63

    Google Scholar 

  • Simpson CG, MacRae E, Gardner RC (1995) Cloning of a polygalacturonase inhibiting protein from Kiwifruit (Actinidia deliciosa). Plant Physiol 108:1748

    Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar, S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Evol 24:1596–1599

    Article  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • Toubart P, Desiderio A, Salvi G et al (1992) Cloning and characterization of the gene encoding the polygalacturonase-inhibiting protein(PGIP) of Phaseolus vulgaris L. Plant J 2:367–373

    PubMed  CAS  Google Scholar 

  • Xu ZL (2010) ABA Rice, cotton, features receptor gene expression, cloning and genetic transformation. Dissertation, Hebei Agricultural University, China

  • Yang CL, Chen ZL et al (1997) LRR proteins of higher plants: structure and function. Biotechnol Prog 17:43–47

    Google Scholar 

  • Zhao WG (2008) Construction of mulberry cDNA library and analysis of genetic diversity. Nanjing University, Nanjing, Postdoctoral thesis

    Google Scholar 

  • Zhang F, Liu X et al (2011) Molecular cloning and characterization of a novel Gossypium barbadense L. RAD-Like gene. Plant Mol Biol Rep 1572–9818

  • Zhao DQ, Zhou CH et al (2010) Molecular cloning and expression of phytoene synthase, lycopene beta-cyclase, and beta-carotene hydroxylase genes in persimmon (Diospyros kaki L.) fruits. Plant Mol Biol Rep 29:345–351

    Article  Google Scholar 

Download references

Acknowledgements

We thank three anonymous reviewers and the editor for critically reviewing the manuscript. This work was supported by China (20060400926), Jiangsu (0602004C) Postdoctoral Science Foundation Project, and Public Industry (Agriculture) Specific Research Program (nyhyzx07-020), and the sericulture industry technology in China program (nycytx-27-gw303).

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Authors and Affiliations

  1. College of Biological and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, 212018, China

    Dongqing Hu, Ruiqiang Dai, Yuhua Wang, Yinghua Zhang, Zhaoyue Liu, Rongjun Fang, Weiguo Zhao, Long Li & Liu Li

  2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, 212018, China

    Dongqing Hu, Ruiqiang Dai, Yuhua Wang, Yinghua Zhang, Zhaoyue Liu, Weiguo Zhao & Long Li

  3. School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210093, China

    Rongjun Fang

  4. General Station of Sericulture Technology Extension of Guangxi Zhuang Autonomous Region, Nanning, 530007, China

    Qiang Lin

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  1. Dongqing Hu
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  2. Ruiqiang Dai
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  3. Yuhua Wang
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  4. Yinghua Zhang
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  5. Zhaoyue Liu
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  7. Weiguo Zhao
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  8. Long Li
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  9. Qiang Lin
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  10. Liu Li
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Correspondence to Weiguo Zhao or Long Li.

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Hu, D., Dai, R., Wang, Y. et al. Molecular Cloning, Sequence Analysis, and Expression of the Polygalacturonase-inhibiting Protein (PGIP) Gene in Mulberry. Plant Mol Biol Rep 30, 176–186 (2012). https://doi.org/10.1007/s11105-011-0324-3

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