Abstract
Glioblastoma multiforme (GBM) is the most malignant brain tumor in humans. Previous studies have demonstrated that microRNA plays important roles in the development and proliferation of GBM cells. Here we defined the mechanism by which miR-212-3p regulated the proliferation of GBM. In this study, we showed that miR-212-3p expression was significantly down-regulated and negatively correlated with serum and glucocorticoid-inducible kinase 3 (SGK3) in GBM. Either over-expression of miR-212-3p or silence of SGK3 decreased viability of GBM cells. Moreover, miR-212-3p directly bound to 3′UTR of SGK3 and inhibited its mRNA and protein expression. And over-expression of SGK3 rescued the decreased proliferation of GBM cells induced by miR-212-3p. Importantly, miR-212-3p also suppressed tumor growth in vivo. Collectively, our results demonstrated that miR-212-3p inhibited proliferation of GBM cells by directly targeting SGK3, and could potentially serve as a new therapeutic target for GBM.
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Acknowledgments
This study was funded by the First Affiliated Hospital of Harbin Medical University Foundation (NO.2014B05 to Huailei Liu, NO.2011BS011 to Bingjie Zheng) and the National Natural Science Foundation of China (NO.81402061 to Huailei Liu, NO.81100854 to Bingjie Zheng, NO. 81372701 and 81172388 to Yaohua Liu, NO.81272788 to Shiguang Zhao).
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None of the authors has any conflict of interest associated with the present work.
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Huailei Liu, Chenguang Li and Chen Shen have contribute equally to this work.
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Liu, H., Li, C., Shen, C. et al. MiR-212-3p inhibits glioblastoma cell proliferation by targeting SGK3. J Neurooncol 122, 431–439 (2015). https://doi.org/10.1007/s11060-015-1736-y
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DOI: https://doi.org/10.1007/s11060-015-1736-y