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Expression of PiABP19, Picdc2 and PiSERK3 during induction of somatic embryogenesis in leaflets of Prunus incisa (Thunb.)

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Abstract

Somatic embryogenesis is a useful tool of plant breeding. In this context, a procedure for inducing somatic embryogenesis in Prunus incisa leaf explants had been previously developed. The original in vitro protocol relies on picloram treatments and exposure to darkness as inductive conditions, the best frequency of embryogenesis being obtained on the second leaf (F2) exposed to 4 μM picloram during 30 days. The morphological and biochemical changes observed during somatic embryogenesis occur in response to alterations in gene expression regulation patterns. A molecular study was conducted in order to provide deeper insight into the fundamental biological factors involved in the induction of this process using a gene candidate strategy and semi-quantitative reverse transcription polymerase chain reaction analysis. So far, no sequence data related to somatic embryogenesis has been available in cherry. In the present study, we cloned and sequenced cDNA fragments of putative genes encoding auxin-binding protein, cell cycle regulator and somatic embryogenesis receptor kinase. Time-course differential transcript accumulations were observed for all investigated genes in leaves or derived callus tissues during the observation period (first month of culture). Their possible involvement in the sequential steps of the embryogenic pathway (dedifferentiation, cell proliferation, differentiation through somatic embryogenesis) is presented and discussed.

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Acknowledgments

This research was carried out with financial support from CGRI (Commissariat Générale des Relations Internationales), Brussels (Belgium). The authors would like to thank Christophe Leroy and Ghassen Abid for technical assistance.

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Authors and Affiliations

  1. National Agronomic Institute of Tunisia, Cité El Mahrajène, 1082, Tunis, Tunisia

    Kaouther Ben Mahmoud

  2. Department of Life Sciences, Bioengineering Unit, Walloon Agricultural Research Centre, Chaussée de Charleroi 234, 5030, Gembloux, Belgium

    Fabienne Delporte, Yordan Muhovski & Philippe Druart

  3. Laboratory of Horticulture, National Agronomic Research Institute of Tunisia, Rue Hédi Karray, 2080, Ariana, Tunisia

    Nadhra Elloumi & Ahmed Jemmali

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  1. Kaouther Ben Mahmoud
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  2. Fabienne Delporte
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  6. Philippe Druart
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Correspondence to Kaouther Ben Mahmoud.

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Ben Mahmoud, K., Delporte, F., Muhovski, Y. et al. Expression of PiABP19, Picdc2 and PiSERK3 during induction of somatic embryogenesis in leaflets of Prunus incisa (Thunb.). Mol Biol Rep 40, 1569–1577 (2013). https://doi.org/10.1007/s11033-012-2205-8

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