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Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)

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Abstract

Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5′-terminal untranslated region (5′-UTR) of 153 bp, and a 3′-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5′-UTR sequence contains an exon and an intron, and the 3′-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic–polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper.

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Acknowledgments

This research was supported by National Basic Research Program of China (2004CB117401), the National Natural Science Foundation of China (U0631007).

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Authors and Affiliations

  1. Pearl River Fishery Research Institute, Chinese Academic of Fishery Sciences, 510380, Guangzhou, China

    Yan Shi, Xin-Ping Zhu & Jing-Kui Yin

  2. Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China

    Qi-Ya Zhang & Jian-Fang Gui

  3. College of Life Sciences, Guangzhou University, 510006, Guangzhou, China

    Xin-Ping Zhu

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  1. Yan Shi
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Corresponding author

Correspondence to Xin-Ping Zhu.

Additional information

The nucleotide sequence data reported in this paper has been submitted to the GenBank under accession number EU877102 for EcIRF-1 cDNA, FJ376610 for EcIRF-1 genomic DNA.

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Shi, Y., Zhu, XP., Yin, JK. et al. Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides). Mol Biol Rep 37, 1483–1493 (2010). https://doi.org/10.1007/s11033-009-9544-0

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  • DOI: https://doi.org/10.1007/s11033-009-9544-0

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