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Apolipoprotein E polymorphism in normal Han Chinese population: frequency and effect on lipid parameters

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Abstract

Apolipoprotein E (ApoE) genotypes were studied in order to determine the prevalence and effect on lipid parameters in normal Han Chinese population. Fragments of ApoE gene forth exon containing codon 112 and 158 polymorphic locus were amplified by PCR, and then digested with Cfo I endonuclease. Genotypes and alleles frequencies of 168 healthy Han Chinese were calculated. The frequency of genotypes ε3/3, ε3/4, and ε2/3 was found to be 75.00, 10.70, and 11.90%, respectively, and 0.60, 1.20, and 0.60% for ε2/2, ε2/4, and ε4/4. The effects of ApoE genotypes and alleles on lipid parameters were analyzed. The effects of ApoE alleles on TC, LDL-C, ApoB was: along a decreasing gradient ε4 > ε3 > ε2. The effect of ε4 allele was to increase serum levels of TC, LDL-C and ApoB, and ε2 allele had an effect opposite to that of ε4 allele. Results obtained in this study indicate that ApoE polymorphism is an independent genetic factor on individual serum levels of lipids and apolipoproteins.

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Abbreviations

ApoAI:

Apolipoprotein AI

ApoB:

Apolipoprotein B

ApoE:

Apolipoprotein E

CHD:

Coronary heart disease

CM:

Chylomicron

HDL-C:

High density lipoprotein-cholesterol

LDL-C:

Low density lipoprotein-cholesterol

Lp(a):

Lipoprotein (a)

TC:

Serum levels of total cholesterol

TG:

Triglyceride

VLDL:

Very low density lipoprotein

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Authors and Affiliations

  1. Department of Neurology, Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215000, People’s Republic of China

    Shu Liang, Jin-Jun Qian & Chun-Feng Liu

  2. Department of Ophthalmology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, People’s Republic of China

    Shu Liang, Hui Chen & Li-Qun Gu

  3. Department of Cardiology, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, People’s Republic of China

    Min Pan, Hai-Hua Geng, Xiao-Tong Qin & Jian-Hua Zhu

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  1. Shu Liang
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  2. Min Pan
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Correspondence to Chun-Feng Liu.

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Shu Liang and Min Pan should both be considered first authors.

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Liang, S., Pan, M., Geng, HH. et al. Apolipoprotein E polymorphism in normal Han Chinese population: frequency and effect on lipid parameters. Mol Biol Rep 36, 1251–1256 (2009). https://doi.org/10.1007/s11033-008-9305-5

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  • DOI: https://doi.org/10.1007/s11033-008-9305-5

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