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Manganese superoxide dismutase expressed in silkworm larvae, Bombyx mori L enhances the NK activity and splenocyte proliferation against Sarcoma 180 tumor cells in vivo

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Abstract

Natural killer cell (NK) is known as a major immune system in body through mediating cell death via several possible pathways, and one of three subpopulations of lymphocytes functioning as scavenger of tumor, virus infected cells etc. Our present results found that the SOD-contained silkworm larvae powder caused an enhancement of the effect on NK cell cytotoxicity, which implied this material modulated the immune system in mice in vivo. The NK cell activities of S180 tumor modeled mice treated with silkworm powder including SOD were enhanced significantly ranging from 30% to 48%, respectively, compare to a distilled water feeding control and silkworm powder without SOD. Meanwhile, the ConA-stimulated splenocyte proliferation of all three treated groups was higher than that of the control both in T cells or B cells. The average tumor weight of S180 modeled mice treated with doses of SOD-contained silkworm powder was lighter than that of water control showing the tumor inhibition rates (IR) reached to 22.51% to 37%, respectively. In conclusion, these findings demonstrate that administration of silkworm larvae powder containing SOD results in activation of NK cells and immune T-cell and B-cell, suggesting the silkworm larvae powder containing SOD play a positive role in tumor inhibition.

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References

  1. Oldham R (1983) Natural killer cells: artifact to reality: an odyssey in biology. Cancer Metastasis Rev 2(4):323–336

    Article  PubMed  CAS  Google Scholar 

  2. Middleton D, Curran M, Maxwell L (2002) Natural killer cells and their receptors. Transpl Immunol 10(2–3):147–164

    Article  PubMed  CAS  Google Scholar 

  3. Harty J, Tvinnereim A, White D (2000) CD8+ T cell effector mechanisms in resistance to infection. Annu Rev Immunol 18:275–308

    Article  PubMed  CAS  Google Scholar 

  4. Kawai T, Akira S (2006) Innate immune recognition of viral infection. Nat Immunol 7(2):131–137

    Article  PubMed  CAS  Google Scholar 

  5. Andersen MH, Schrama D, Thor Straten P, Becker JC (2006) Cytotoxic T cells. J Invest Dermatol 126(1):32–41

    Article  PubMed  CAS  Google Scholar 

  6. Boon T, van der Bruggen P (1996) Human tumor antigens recognized by T lymphocytes. J Exp Med 183: 725–729

    Article  PubMed  CAS  Google Scholar 

  7. Guevara-Patino JA, Turk MJ, Wolchok JD, Houghton AN (2003) Immunity to cancer through immune recognition of altered self: studies with melanoma. Adv Cancer Res 90:157–177

    Article  PubMed  CAS  Google Scholar 

  8. Renkvist N, Castelli C, Robbins PF, Parmiani G (2001) A listing of human tumor antigens recognized by T cells. Cancer Immunol Immunother 50:3–15

    Article  PubMed  CAS  Google Scholar 

  9. Weisiger RA, Fridovich I (1973) Superoxide dismutase: organelle specificity. J Biol Chem, 248:3582–3592

    PubMed  CAS  Google Scholar 

  10. Sandstrom J, Nilsson P, Karlsson K (1994) 10 fold increase in human plasma extracellular superoxide dismutase content caused by a mutation in heparin-binding domain. J Biol Chem 269(29):19163–19166

    PubMed  CAS  Google Scholar 

  11. Yue WF, Miao YG, Li XH, Wu XF, Zhao AC, Nakagaki M (2006) Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-Bac/BmNPV Baculovirus expression system. Appl Microbiol Biotechnol 73:181–186

    Article  PubMed  CAS  Google Scholar 

  12. Yue W-F, Li G-L, Liu J-M, Sun J-T, Sun H-X, Li X-H, Wu X-F, Miao Y-G (2007) Anti-oxidation and immune responses in mice upon exposure to manganese superoxide dismutase expressed in silkworm larvae, Bombyx mori L. Cell Biol Int 31:974–978

    Article  PubMed  CAS  Google Scholar 

  13. Yue W-F, Liu J-M, Sun J-T, Li G-L, Li X-H, Wu X-F, Sun H-X, Zhou J-Y, Miao Y-G (2007) Immunity promotion and proteomic identification in mice upon exposure to manganese superoxide dismutase expressed in silkworm larvae. J Proteome Res 6(5):1875–1881

    PubMed  CAS  Google Scholar 

  14. Pan HJ, Sun HX, Pan YJ (2005) Adjuvant effect of ethanol extract of Semen Cuscutae on the immune responses to ovalbumin inmice. J Ethnopharmacol 99:99–103

    Article  PubMed  Google Scholar 

  15. Chandra RK (1997) Nutrition and the immune system: an introduction. Am J Clin Nutr 66:460S–463S

    PubMed  CAS  Google Scholar 

  16. Sproul T, Cheng P, Dykstra M, Pierce S (2000) A role for MHC class II antigen processing in B cell development. Int Rev Immunol 19(2–3):139–155

    Article  PubMed  CAS  Google Scholar 

  17. Middleton D, Curran M, Maxwell L (2002) Natural killer cells and their receptors. Transpl Immunol 10(2–3):147–164

    Article  PubMed  CAS  Google Scholar 

  18. Holtmeier W, Kabelitz D (2005) Gammadelta T cells link innate and adaptive immune responses. Chem Immunol Allergy 86:151–183

    Article  PubMed  CAS  Google Scholar 

  19. Keller MA, Stiehm ER (2000) Passive Immunity in Prevention and Treatment of Infectious Diseases. Clin Microbiol Rev 13(4):602–614

    Article  PubMed  CAS  Google Scholar 

  20. Louvel E, Hugon J, Doble A (1997) Therapeutic advances in amyotrophic lateral sclerosis. Trends Pharm Sci, 18:196–203

    PubMed  CAS  Google Scholar 

  21. Maier CM, Chan PH (2002) Role of superoxide dismutases in oxidative damage and neurodegenerative disorders. Neuroscientist 8:323–334

    Article  PubMed  CAS  Google Scholar 

  22. Maeda S (1994) Expression of foreign genes in insect cells using baculovirus vectors. In: Maramorosch K, McIntosh AH (eds) Insect cell biotechnology. CRC Press, p 1–31

  23. Smyth MJ, Cretney E, Kelly JM, Westwood JA, Street SE, Yagita H (2005) Activation of NK cell cytotoxicity. Mol Immunol 42:501–510

    Article  PubMed  CAS  Google Scholar 

  24. Frumento G, Piazza T, Di Carlo E, Ferrini S (2006) Targeting tumor-related immunosuppression for cancer immunotherapy. Endocr Metab Immune Disord Drug Targets 6(3):233–237

    Article  PubMed  Google Scholar 

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Acknowledgment

This work was supported by the Hi-Tech Research and Development Program of China (No. 2006AA10A119).

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Authors and Affiliations

  1. Institute of Sericulture and Apiculture, Zhejiang University, Hangzhou, 310029, P.R. China

    Wan-Fu Yue, Jian-Mei Liu, Guang-Li Li, Xing-Hua Li, Xiao-Feng Wu & Yun-Gen Miao

  2. College of Medicine, Zhejiang Chinese Medical University, Hangzhou, 310053, P.R. China

    Min-Li Yao

  3. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310029, P.R. China

    Wen Deng, Hong-Xiang Sun & Ji-Yong Zhou

  4. Institute of Insect Sciences, Zhejiang University, Hangzhou, 310029, P.R. China

    Chuan-Xi Zhang

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  1. Wan-Fu Yue
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  11. Yun-Gen Miao
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Correspondence to Yun-Gen Miao.

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Yue, WF., Yao, ML., Liu, JM. et al. Manganese superoxide dismutase expressed in silkworm larvae, Bombyx mori L enhances the NK activity and splenocyte proliferation against Sarcoma 180 tumor cells in vivo. Mol Biol Rep 36, 187–192 (2009). https://doi.org/10.1007/s11033-007-9165-4

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  • DOI: https://doi.org/10.1007/s11033-007-9165-4

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