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Cereal cyst nematode resistance gene CreV effective against Heterodera filipjevi transferred from chromosome 6VL of Dasypyrum villosum to bread wheat

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Abstract

Cereal cyst nematodes (CCN) are a global economic problem for cereal production. Heterodera filipjevi is one of the most commonly identified and widespread CCN species found in many wheat production regions of the world. Transferring novel genes for resistance to H. filipjevi from wild relatives of wheat is a promising strategy for protection of wheat crops. A set of wheat–Dasypyrum villosum chromosome addition lines, T6V#4S·6AL translocation lines and their donor parental lines were tested for their response to the nematode. D. villosum and wheat–D. villosum disomic addition line DA6V#4 were resistant. As T6V#4S·6AL translocation lines were susceptible, resistance was presumed to be located on chromosome 6V#4L. The objective of this study was to produce and characterize wheat–6V#4L translocations and confirm the chromosome location of the resistance. Introgression lines T6V#4L·6AS, T6V#4L-4BL·4BS and DT6V#4L were developed and subjected to molecular cytogenetic analysis. These and four additional wheat–6V#4 introgression lines were tested for response to H. filipjevi in the greenhouse. The results indicated that introgression lines DA6V#4, T6V#4L·6AS, T6V#4L-4BL·4BS, T6V#4L·6V#4S-7BS and DT6VL#4 had higher levels of H. filipjevi resistance than their recurrent parent. However, Del6V#4L-1 and translocation line T6V#4S·6AL were equally susceptible to wheat cv. Chinese Spring. The CCN resistance gene, temporarily named CreV, was therefore physically mapped to chromosome arm 6V#4L FL 0.80–1.00. Translocation chromosomes T6V#4L·6AS transferred to a modern wheat cv. Aikang 58 with its co-dominant molecular markers could be utilized as a novel germplasm for CCN resistance breeding in wheat.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Project no. 30871519) and The 863 High-tech Program (Project no. 2012AA101105). The authors would like to thank Dr. Ian Riley, Australia and Prof. Bob McIntosh, the University of Sydney Plant Breeding Institute Cobbitty, NSW, Australia, for their comments on the manuscript.

Author contributions

Conceived and designed the experiments: Ruiqi Zhang; Developed translocation lines NAU424 and NAU426: Yigao Feng; Developed the translocation line NAU423: Haifeng Li; Identified the CCN resistance: Hongxia Yuan, Junli Dai and Honglian Li; Wrote the paper: Ruiqi Zhang, Aizhong Cao and Liping Xing.

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Authors and Affiliations

  1. College of Agronomy, Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/JCIC-MCP, Nanjing, 210095, Jiangsu, China

    Ruiqi Zhang, Yigao Feng, Haifeng Li, Aizhong Cao & Liping Xing

  2. College of Plant Protection, Henan Agricultural University/National Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops, Zhengzhou, 450002, Henan, China

    Hongxia Yuan, Junli Dai & Honglian Li

  3. Yangzhou Polytechnic College, Yangzhou, 225009, Jiangsu, China

    Haifeng Li

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  1. Ruiqi Zhang
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  2. Yigao Feng
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  4. Hongxia Yuan
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  8. Honglian Li
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Correspondence to Ruiqi Zhang or Honglian Li.

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Supplemental Figure S1

PCR patterns of markers in cv. Chinese Sprint, D. villosum, Triticum durum cv. ZY1286–D. villosum amphiploid (AABBVV), T. aestivum–D. villosum disomic substitution line DS6V#4 (6A), translocation line T6V#4S·6AL, homozygous translocation lines NAU423 and NAU424 and nullisomic 6A–tetrasomic 6B (N6AT6B). The straight line (right) indicates the fragments that can be assigned to a certain chromosome. a PCR pattern of 6L-4. b PCR pattern of SSR marker WMC256. c PCR pattern of SSR marker GWM570. d PCR pattern of CINAU15. Translocation line NAU423 harbors the 6VL- and 6AS-specific fragments, but is missing the 6VS-specific and 6AL-specific fragments, indicating they are homozygous for T6V#4S·6AL. (TIFF 1082 kb)

Supplemental Figure S2

a Plants of T6V#4S·6AL translocation line 14zrq7-29-1 (left) and Aikang 58 (right). b Spikes of T6V#4S·6AL translocation line 14zrq7-29-1 (left) and Aikang 58 (right. c Seeds of T6V#4S·6AL translocation line 14zrq7-29-1 (left) and Aikang 58 (right). d Root phenotype of Aikang 58 after infected with H. filipjevi; arrow indicates the CCN eggs attached on the root resulting in abnormal branching. e Root phenotype of T6V#4S·6AL translocation line 14zrq7-29-1 after infection with H. filipjevi. (TIFF 2644 kb)

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Zhang, R., Feng, Y., Li, H. et al. Cereal cyst nematode resistance gene CreV effective against Heterodera filipjevi transferred from chromosome 6VL of Dasypyrum villosum to bread wheat. Mol Breeding 36, 122 (2016). https://doi.org/10.1007/s11032-016-0549-9

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