Abstract
Zinc deficiency in humans is recognized as a widespread public health problem worldwide, but can be combated via genetic biofortification through breeding high zinc containing wheat varieties. CIMMYT (International Maize and Wheat Improvement Center, Int.) is engaged in enhancing, among others, the grain zinc concentration (GZnC) of high-yielding wheat germplasm under the HarvestPlus initiative of the Consultative Group on International Agricultural Research consortium. In the present study, we determined GZnC in a recombinant inbred line population from the cross between PBW343 and Kenya Swara in replicated trials grown in Zn-enriched field. An integrated genetic map with 1,133 loci (diversity arrays technology and simple sequence repeats markers) covering all 21 wheat chromosomes was constructed and used for quantitative trait loci (QTL) analysis. Two novel QTL of large effect were stably detected for increasing GZnC on chromosomes 2Bc (centromeric region) and 3AL (long arm). The two QTL individually explained about 10–15 % of the total phenotypic variation. The 2Bc QTL from PBW343 have pleiotropic effect and can increase thousand-kernel weight at significant level. The QTL and the closely linked markers identified will make selection for this difficult trait feasible in breeding program.
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AACC International (2010) Approved methods of analysis, 11th edn. Method 55-1001 test weight per bushel. AACC International, St. Paul, MN, USA. doi:10.1094/AACCIntMethod-55-1001
Andreini C, Banci L, Bertini I, Rosato A (2006) Zinc through the three domains of life. J Proteome Res 5:3173–3178
Black RE, Victora CG, Walker SP, Bhutta ZA, Christian P, de Onis M, Ezzati M, Grantham-McGregor S, Katz J, Martorell R, Uauy R (2013) Maternal and child undernutrition and overweight in low-income and middle-income countries. Lancet 382:427–451
Bouis HE, Welch RM (2010) Biofortification—a sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop Sci 50:S20–S32
Bouis HE, Hotz C, McClafferty B, Meenakshi J, Pfeiffer WH (2011) Biofortification: a new tool to reduce micronutrient malnutrition. Food Nutr Bull 32:31S–40S
Braun HJ, Atlin G, Payne T (2010) Multi-location testing as a tool to identify plant response to global climate change. In: Reynolds MP (ed) Climate change and crop production. CABI Press, Oxford, pp 115–138
Broadley MR, White PJ, Hammond JP, Zelko I, Lux A (2007) Zinc in plants. New Phytol 173:677–702
Broman KW, Wu H, Sen Ś, Churchill GA (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19:889–890
Cakmak I (2000) Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol 146:185–205
Cakmak I (2008) Enrichment of cereal grains with zinc: agronomic or genetic biofortification? Plant Soil 302:1–17
Cakmak I (2012) HarvestPlus zinc fertilizer project: HarvestZinc. Better Crop 96:17–19
Cakmak I, Ozkan H, Braun H, Welch R, Romheld V (2000) Zinc and iron concentrations in seeds of wild, primitive, and modern wheats. Food Nutr Bull 21:401–403
Chasapis C, Loutsidou A, Spiliopoulou C, Stefanidou M (2012) Zinc and human health: an update. Arch Toxicol 86:521–534
Graham RD, Welch RM, Bouis HE (2001) Addressing micronutrient malnutrition through enhancing the nutritional quality of staple foods: principles, perspectives and knowledge gaps. Adv Agron 70:77–142
Graham R, Knez M, Welch R (2012) How much nutritional iron deficiency in humans globally is due to an underlying zinc deficiency? Adv AgronAdv Agron 115:1–40
Hao Y, Chen Z, Wang Y, Bland D, Buck J, Brown-Guedira G, Johnson J (2011) Characterization of a major QTL for adult plant resistance to stripe rust in US soft red winter wheat. Theor Appl Genet 123:1401–1411
Hershfinkel M (2006) Zn2+, a dynamic signaling molecule. In: Tamas M, Martinoia E (eds) Molecular biology of metal homeostasis and detoxification. Springer, Berlin, pp 131–153
Jiang Q, Hou J, Hao C, Wang L, Ge H, Dong Y, Zhang X (2011) The wheat (T. aestivum) sucrose synthase 2 gene (TaSus2) active in endosperm development is associated with yield traits. Funct Integr Genomics 11:49–61
Krämer U, Clemens S (2006) Functions and homeostasis of zinc, copper, and nickel in plants. In: Tamas M, Martinoia E (eds) Molecular biology of metal homeostasis and detoxification. Springer, Berlin, pp 216–271
Lopes M, Reynolds M, Jalal-Kamali M, Moussa M, Feltaous Y, Tahir I, Barma N, Vargas M, Mannes Y, Baum M (2012) The yield correlations of selectable physiological traits in a population of advanced spring wheat lines grown in warm and drought environments. Field Crop Res 128:129–136
Lorenz AJ, Chao S, Asoro FG, Heffner EL, Hayashi T, Iwata H, Smith KP, Sorrells ME, Jannink JL (2011) Genomic selection in plant breeding: knowledge and prospects. Adv Agron 110:77–123
Monasterio I, Graham RD (2000) Breeding for trace minerals in wheat. Food Nutr Bull 21:392–396
Murphy KM, Reeves PG, Jones SS (2008) Relationship between yield and mineral nutrient concentrations in historical and modern spring wheat cultivars. Euphytica 163:381–390
Paltridge NG, Milham PJ, Ortiz-Monasterio JI, Velu G, Yasmin Z, Palmer LJ, Guild GE, Stangoulis JCR (2012) Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat. Plant Soil 361:261–269
Peterson C, Johnson V, Mattern P (1983) Evaluation of variation in mineral element concentrations in wheat flour and bran of different cultivars. Cereal Chem 60:450–455
Pfeiffer WH, McClafferty B (2007) HarvestPlus: breeding crops for better nutrition. Crop Sci 47:S88–S105
Randhawa HS, Mutti JS, Kidwell K, Morris CF, Chen X, Gill KS (2009) Rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection. PLoS ONE 4:e5752
Rink L (2011) Zinc in human health Amsterdam. IOS Press, Netherlands
Singh R, Huerta-Espino J, Sharma R, Joshi A, Trethowan R (2007) High yielding spring bread wheat germplasm for global irrigated and rainfed production systems. Euphytica 157:351–363
Singh S, Singh RP, Bhavani S, Huerta-Espino J, Eugenio L-VE (2013) QTL mapping of slow-rusting, adult plant resistance to race Ug99 of stem rust fungus in PBW343/Muu RIL population. Theor Appl Genet 126:1367–1375
Velu G, Ortiz-Monasterio I, Singh RP, Payne T (2011) Variation for grain micronutrients concentration in wheat core-collection accessions of diverse origin. Asian J Crop Sci 3:43–48
Velu G, Singh R, Huerta-Espino J, Peña R, Arun B, Mahendru-Singh A, Mujahid MY, Sohu V, Mavi G, Crossa J (2012) Performance of biofortified spring wheat genotypes in target environments for grain zinc and iron concentrations. Field Crop Res 137:261–267
Velu G, Ortiz-Monasterio I, Cakmak I, Hao Y, Singh RP (2013) Biofortification strategies tþo increase grain zinc and iron concentrations in wheat. J Cereal Sci. doi:10.1016/j.jcs2013.09.001
Wang S, Basten CJ, Zeng ZB (2012) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm
Wessells KR, Brown KH (2012) Estimating the global prevalence of zinc deficiency: results based on zinc availability in national food supplies and the prevalence of stunting. PLoS ONE 7:e50568
WHO (2012) The world health report Geneva. World Health Organization Press, Switzerland
Xu Y, An D, Li H, Xu H (2011) Breeding wheat for enhanced micronutrients. Can J Plant Sci 91:231–237
Zanetti S, Winzeler M, Feuillet C, Keller B, Messmer M (2001) Genetic analysis of bread-making quality in wheat and spelt. Plant Breed 120:13–19
Acknowledgments
The authors acknowledge financial support from the HarvestPlus Challenge Program under Agreement No. 5212 to CIMMYT. Editing assistance from Emma Quilligan (CIMMYT) is appreciated.
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The authors declare that there are no conflicts of interest.
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Yuanfeng Hao and Govindan Velu have contributed equally to this work.
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Hao, Y., Velu, G., Peña, R.J. et al. Genetic loci associated with high grain zinc concentration and pleiotropic effect on kernel weight in wheat (Triticum aestivum L.). Mol Breeding 34, 1893–1902 (2014). https://doi.org/10.1007/s11032-014-0147-7
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DOI: https://doi.org/10.1007/s11032-014-0147-7