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Development of SCAR Markers for Germplasm Characterisation in Olive Tree (Olea europea L.)

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An Erratum to this article was published on 01 April 2006

Abstract

A set of 14 SCAR markers were developed starting from RAPD, AFLP and SAMPL analysis of several olive germplasm accessions. Eight RAPD, two AFLP and four SAMPL fragments were converted into dominant and codominant SCARs by cloning and sequencing the selected fragments. The markers obtained were evaluated on forty different olive cultivars from different Italian production areas (mainly from Liguria). The combined use of these SCARs made possible to univocally identify 26 cultivars while the remaining 14 will require the development of further markers since most of them are placed in a main group containing six genetically similar cultivars (among which Frantoio and Taggiasca) and four minor groups containing two cultivars each. A total of 31 different haplotypes were identified and the analysis of several individual plants indicated no intra-cultivar variability. Considering the SCAR polymorphism two alleles were scored for each markers with the only exception of markers IGPS3 and IGPS4 showing 4 alleles with 7 recognised groups and 5 alleles with 4 groups, respectively. Though less polymorphic in comparison with other markers like SSRs, the developed SCARs proved useful in genotype identification. In addition, they could potentially be used for breeding applications and forensic analysis.

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Authors and Affiliations

  1. Instituto di Botanica e, Genetica Vegetale, Università Cattolica Sacro Cuore, via Emilia Parmense, 84, 29100, Piacenza, Italy

    Matteo Busconi, Luca Sebastiani & Corrado Fogher

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  1. Matteo Busconi
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  2. Luca Sebastiani
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  3. Corrado Fogher
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Correspondence to Matteo Busconi.

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An erratum to this article is available at http://dx.doi.org/10.1007/s11032-006-8120-8.

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Busconi, M., Sebastiani, L. & Fogher, C. Development of SCAR Markers for Germplasm Characterisation in Olive Tree (Olea europea L.). Mol Breeding 17, 59–68 (2006). https://doi.org/10.1007/s11032-005-1395-3

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  • DOI: https://doi.org/10.1007/s11032-005-1395-3

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