Abstract
Resveratrol has attracted considerable public and scientific attention because of its beneficial effects on human health. In this study, resveratrol was encapsulated into chitosan microspheres for improving the stability and evaluating the ability of controlled release. Chitosan microspheres were prepared by using an emulsification-crosslinking method, and specifically, a natural extraction genipin was used as crosslinking agent for pursuit better physiological compatibility. Factors which influence on the shape and dispersity of microspheres were investigated to optimize the preparing process of resveratrol loaded genipin-chitosanmicrospheres. The optimum condition was obtained and the prepared resveratrol loaded genipin-chitosanmicrospheres were characterized by many technical instruments. Entrapment efficiency of resveratrol in genipin-chitosanmicrospheres is up to 87.6 %, and loading capacity is 7.9 %. The in vitro release rate in pH 3.6 buffer solution reached to 33.8 % within 72 h, but was lower in pH 7.4 buffer solution.
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References
Robards K (2003) J Chromatogr A 1000:657–691
Lucio M, Roberto MP, Tesei I (2006) J Chromatogr A 1114:263–268
Claudine M, Gary W, Christine M (2005) J Clin Nutr 81:230S–242S
Frémont L (2000) Life Sci 66:663–673
Gusman J, Malonne H, Atassi G (2001) Carcinogenesis 22:1111–1117
Cai LS, Koziel JA, Dharmadhikari M (2009) J Chromatogr A 1216:281–287
Juan ME, Alfaras I, Planas JM (2012) Pharmacol Res 65:584–591
Siemann EH, Creasy LL (1992) Am J Enol Vitic 43:49–52
Soleas GJ, Diamandis EP, Goldberg DM (1997) Clin Biochem 30:91–113
Luan TG, Li GK, Zhang ZX (2000) Anal Chim Acta 424:19–25
Lucio M, Roberto P (2008) J Chromatogr A 1185:23–30
Athar M, Back JH, Tang X (2007) Toxicol Appl Pharmacol 224:274
Stocco B, Toledo K, Salvador M (2012) Maturitas 72:72–78
Peng HL, Xiong H, Li JH (2010) Food Chem 121:23–28
Kim YS, Sull JW, Sung HJ (2012) Mol Biol Rep 39:8709–8716
Piotrowska H, Kucinska M, Murias M (2012) Mutat Res 750:60–82
Timothy HS, Robert WM, Jr, Keith WH (2000) J Agric Food Chem 48:1243–1246
Clark D, Tuor UI, Thompson R (2012) PLoS One 7:e47792
Goldberg DM (1995) Clin Chem 41:14–16
Zhang Y, Zhang YY, Yu CJ (2012) Adv Mater Res 479–481:833–836
Signorelli P, Ghidoni R (2005) J Nutr Biochem 16:449–466
Lu Z, Cheng B, Hu YL (2009) Food Chem 113:17–20
Juan ME, Buenafuente J, Casals I (2002) Food Res Int 35:195–199
Babazadeh M, Edjlali L, Rashidian L (2007) J Polym Res 14:207–213
Oh A, Yun JM, Kim H (2011) J Polym Res 18:2441–2447
Vidgren P, Vidgren M, Arppe J (1992) Drug Dev Ind Pharm 18:581–597
Anal AK, Stevens WF, Remuňán-López C (2006) Int J Pharm 312:166–173
Gao JG, Zhang Y, Yu YF (2011) J Polym Res 18:1501–1508
Liu ZY, Ge XJ, Lu Y (2012) Food Hydrocoll 26:311–317
Chiu FC, Lai SM, Hsieh IC (2012) J Polym Res 19:9781–9785
Casettari L, Vllasaliu D, Lam JKW (2012) Biomaterials 33:7565–7583
Xu JH, Li SW, Tostado C (2009) Biomed Microdevices 11:243–249
Ma LH, Liu CS (2010) Colloids Surf B Biointerfaces 75:448–453
Zhang YL, Wei W, Lv PP (2011) Eur J Pharm Biopharm 77:11–19
Zhang C, Cheng Y, Qu GW (2008) Carbohydr Polym 72:390–397
Gupta KC, Jabrail FH (2006) Carbohydr Polym 66:43–54
Kawadkar J, Chauhan MK (2012) Eur J Pharm Biopharm 81:563–572
Harris R, Lecumberri E, Heras A (2010) Mar Drugs 8:1750–1762
Karnchanajindanun J, Srisa-ard M, Baimark Y (2011) Carbohydr Polym 85:674–680
Aldana AA, González A, Strumia MC (2012) Mater Chem Phys 134:317–324
Chinese Pharmacopoeia (2010) The guiding principles for stability test of drugs and drug preparations: appendix XIX C
Chinese Pharmacopoeia (2010) The guiding principles for sustained release, controlled release and delayed released of Pharmaceutical preparations: appendix XIX D
Chen XL, Zhao GW, Liao ZG (2010) Proceedings of academic development BBS of Chinese medicine in Jiangxi Province: 273–279
Agnihotri SA, Mallikarjuna NN, Aminabhavi TM (2004) J Control Release 100:5–28
Acknowledgments
The authors would like to thank the mutual funds of Hebei Natural Science Foundation and CSPC Pharmaceutical Co., Ltd. (C2011208114). Supports for Hebei Research Center of Pharmaceutical and Chemical Engineering, Hebei Province Key Laboratory of Molecular Chemistry for Drug, and Innovation Team Funding of Hebei University of Science and Technology are also appreciated.
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Zhang, Y., Yu, Yf., Shi, Xx. et al. Study on the preparation of genipin crosslinked chitosan microspheres of resveratrol and in vitro release. J Polym Res 20, 175 (2013). https://doi.org/10.1007/s10965-013-0175-8
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DOI: https://doi.org/10.1007/s10965-013-0175-8