Abstract
We performed a comprehensive metaproteomic analysis of the dissolved organic matter (DOM) in Japanese coastal waters using liquid chromatography–tandem mass spectrometry and demonstrated that these proteomes were characterized by proteins with various functions, including metabolic enzymes, membranes, and photosynthetic proteins. The protein sources included cyanobacteria, heterotrophic bacteria, and eukaryotic phytoplankton. Most of the components were similar among samples and also similar to pelagic components. We also observed differences in the compositions of the microbial communities of origin among the different dissolved protein samples and differences in the relative abundance of specific dissolved protein types (e.g., cytoskeletal proteins), possibly indicating potential dynamics in the coastal DOM pool.
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Acknowledgments
This study was performed under the “Global COE Program in Ehime University” of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan and was partially supported by the Sasagawa Scientific Research Grant (no. 23-728) from the Japan Science Society and a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS).
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Yoshida, M., Yamamoto, K. & Suzuki, S. Metaproteomic characterization of dissolved organic matter in coastal seawater. J Oceanogr 70, 105–113 (2014). https://doi.org/10.1007/s10872-013-0212-6
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DOI: https://doi.org/10.1007/s10872-013-0212-6