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Triple-FRET Technique for Energy Transfer Between Conjugated Polymer and TAMRA Dye with Possible Applications in Medical Diagnostics

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Abstract

Three-component Förster resonance energy transfer (FRET) has been used to obtain efficient FRET between the cationic conjugated polymer (CCP) as donor and 5-carboxy tetramethylrhodamine (TAMRA) dye as acceptor, by using an intermediate donor, fluorescein. In spite of the fact that there is enough overlap between the emission spectra of CCP and absorption spectra of TAMRA, the efficiency of FRET between CCP and TAMRA is poor. The reason for this is that while the Förster critical distance is not very sensitive to the overlap, the FRET efficiency is extremely sensitive to it. However, it is observed that the FRET efficiency between CCP and TAMRA improves considerably when fluorescein is introduced in the solution. The triple FRET so obtained can be used for deoxyribonucleic acid sequence detection in medical diagnostics because the fluorescence emission from TAMRA is pH-insensitive.

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References

  1. Gaylord, B.S., Heeger, A.J., Bazan, G.C.: DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes. Proc. Natl. Acad. Sci. USA 99(17), 10954–10957 (2002)

    Article  ADS  Google Scholar 

  2. Bhatnagar, P.K., Mathur, P.C., Mathur, N., Aneja, A.: Low cost portable sensors for medical diagnostics using Förster resonance energy transfer between water soluble cationic conjugated polymers and PNAC* probes. Paper Presented at International Conference on Materials for Advanced Technologies (ICMAT), Singapore 1–6 July (2007)

  3. Behlke, M.A., Huang, L., Bogh, L., Rose, S., Devor, E.J.: Fluorescence and fluorescence applications. Integrated DNA Technologies. http://www.idtdna.com/support/technical/TechnicalBulletinPDF/fluorescence_and_fluorescence_applications.pdf (2005)

  4. Cardullo, R.A., Agrawal, S., Flores, C., Zamechnik, P.C., Wolf, D.E.: Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer. Proc. Natl. Acad. Sci. USA 85, 8790–8794 (1988)

    Article  ADS  Google Scholar 

  5. Mocz, G.: Fluorescence resonance energy transfer (FRET) as a probe of proximity in proteins. http://dwb.unl.edu/Teacher/NSF/C08/C08Links/pps99.cryst.bbk.ac.uk/projects/gmocz/fret.htm (1999)

  6. Herman, B., Centonze, F.V.E., Lakowicz, J.R., Fellers, T.J., Davidson, M.W.: Applications in confocal microscopy, fluorescence resonance energy transfer (FRET) microscopy. Olympus (FRET literature sources). http://www.olympusfluoview.com/applications/images/fretintrofigure2.jpg&imgrefurl (2004)

  7. Cambridge Research Biochemicals: Cambridge Research Biochemicals expands dye offering. Cambridge Research Biochemicals. http://www.crbdiscovery.com/news/article_sep07.php (2007)

  8. Invitrogen: The Handbook—A Guide to Fluorescent Probes and Labeling Technologies, Section 1.5 – Fluorescein, Oregon Green and Rhodamine Green Dyes, http://probes.invitrogen.com/handbook/sections/0105.html (2005)

  9. Sano, S., Tomizaki, K.-Y., Usui, K., Mihara, H.: A PNA–DNA hybridization chip approach for the detection of β-secretase activity. Bioorg. Med. Chem. Lett. 16(3), 503–506 (2006)

    Article  Google Scholar 

  10. Haustein, E., Jahnz, M., Schwille, P.: Triple FRET: a tool for studying long-range molecular interactions. ChemPhysChem 4, 745–747 (2003)

    Article  Google Scholar 

  11. Song, X., Shi, J., Swanson, B.: Detection of multivalent interactions through two-tiered energy transfer. Anal. Biochem. 291, 133–141 (2001)

    Article  Google Scholar 

  12. Semrock: Fluorescence resonance energy transfer (FRET). http://www.semrock.com/Catalog/BrightLineFRET.htm (2007)

  13. Powell, J.: FRET lecture. Reed College, Portland, OR. http://people.reed.edu/~dna/1-fret-07dec03%20copy.pdf (2003)

  14. Heather, M.W., Chia-Pin, P., Mary, D.B.: Two-step FRET as a structural tool. J. Am. Chem. Soc. 125, 7336–7343 (2003)

    Article  Google Scholar 

  15. Dogariu, A., Gupta, R., Heeger, A.J., Wang, H.: Time-resolved Förster energy transfer in polymer blends. Synth. Metals 100, 95–100 (1999)

    Article  Google Scholar 

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Acknowledgements

Financial support from the Department of Biotechnology (DBT), India, (BT/PR6440/MED/14/812/2005) is gratefully acknowledged. Authors also wish to thank Dr. Rani Gupta and Prof. R.K Saxena (Dept. of Microbiology, University of Delhi South Campus, New Delhi) for their helpful suggestions.

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  1. Materials Laboratory, Department of Electronic Science, University of Delhi, South Campus, Benito Juarez Road, New Delhi, 110021, India

    Anamika Aneja, Nidhi Mathur, P. K. Bhatnagar & P. C. Mathur

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  1. Anamika Aneja
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  2. Nidhi Mathur
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  3. P. K. Bhatnagar
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  4. P. C. Mathur
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Correspondence to Anamika Aneja.

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Aneja, A., Mathur, N., Bhatnagar, P.K. et al. Triple-FRET Technique for Energy Transfer Between Conjugated Polymer and TAMRA Dye with Possible Applications in Medical Diagnostics. J Biol Phys 34, 487–493 (2008). https://doi.org/10.1007/s10867-008-9107-y

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  • DOI: https://doi.org/10.1007/s10867-008-9107-y

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