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Chondrogenic effect of cell-based scaffold of self-assembling peptides/PLGA-PLL loading the hTGFβ3 plasmid DNA

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

With the application of tissue engineering to tissue regeneration, additional new complexes have been made in response to the challenge of cartilage-injury repair. This study was performed to construct a rat precartilaginous stem cells-based scaffold of self-assembling peptides RADA16-I/PLGA-PLL (poly-l-lysine coated PLGA) as extracellular matrix loading the NLS-TAT as a peptide-based carrier for a plasmid DNA containing hTGFβ3. After composites were cultured for 1, 2, 3 and 4 weeks, respectively, the results showed that the levels of chondrogenic-related gene expression were higher in the experimental group with and hTGFβ3 gene by reverse transcription-polymerase chain reaction, and with higher histochemical and immunohistochemical expression. hTGFβ3 protein expression had increased at 4 weeks based on western blot analysis. The results of this study show that a complex may be a suitable scaffold for cartilage repair and offer a strategy for tissue regeneration through the use of tissue engineering.

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Acknowledgments

This work was supported by Grants from the Nature Science Foundation of Hubei Province (No. 2014060101010048) and National Natural Science Foundation of China (No. 81541020).

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Authors and Affiliations

  1. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China

    Qiyong Pan, Wenkai Li, Xuefeng Yuan, Yeltay Rakhmanov, Pengcheng Wang, Rui Lu, Zekai Mao, Xiaobin Shang & Hongbo You

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Pan, Q., Li, W., Yuan, X. et al. Chondrogenic effect of cell-based scaffold of self-assembling peptides/PLGA-PLL loading the hTGFβ3 plasmid DNA. J Mater Sci: Mater Med 27, 19 (2016). https://doi.org/10.1007/s10856-015-5631-z

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