Abstract
An attempt was made to induce rooting from single node cuttings of Camellia sinensis var. TV-20 under controlled conditions and study its biochemical changes during rooting. The nodal cuttings were pretreated with different concentrations of IAA, NAA and IBA and kept in a growth chamber (25 ±2 °C, 16 h photoperiod (55 μ mol m−2 s−1) with cool, white fluorescent lamps and 65% relative humidity) for 12 h. Among the three auxins used for pretreatment, IBA showed more positive response on rooting as compared to IAA and NAA within 2 weeks of transfer to potting medium. Among four concentrations of IBA tested, 75 ppm gave maximum percentage of rooting, number of roots and root length. Therefore, IBA was used further in experiments for biochemical investigation. The adventitious rooting was obtained in three distinct phases i.e. induction (0–12 days), initiation (12–14 days) and expression (14–18 days). IAA-oxidase activity of IBA-treated cuttings increased slightly as compared to control. The activity was found to decrease during induction and initiation phases and increase during expression phase. The peroxidase activity in IBA-treated cuttings increased up to initiation phase and declined at the expression phase. Polyphenoloxidase activity increased both in IBA-treated and control cuttings during induction and initiation phase but declined slowly during expression phase. Total phenolic content was higher in IBA-treated cuttings, particularly in initiation and expression phases and it also correlated with peroxidase activity. Phenolics might be playing key role for induction of adventitious rooting, and phenolic compounds can be used as rooting enhancer in tea plant.
Similar content being viewed by others
Abbreviations
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- NAA:
-
1-naphthaleneacetic acid
References
InstitutionalAuthorNameAnonymous (1976) The Wealth of India NumberInSeriesVol. II Council of Scientific Industrial Research New Delhi
R.A. Aeschbacher J.W. Schiefelbein P.N. Benfey (1994) ArticleTitleThe genetic and molecular basis of root development Annu. Rev. Plant Physiol. Plant Mol. Biol. 45 25–45 Occurrence Handle10.1146/annurev.pp.45.060194.000325 Occurrence Handle1:CAS:528:DyaK2cXlsFehsrw%3D
M. Banerjee B. Agarwal (1990) ArticleTitleIn vitro rooting teaCamellia sinensis (L.) O.Kuntze Ind. J. Expt. Biol. 28 IssueID10 936–939 Occurrence Handle1:CAS:528:DyaK3MXns12iug%3D%3D
M.M. Bradford (1976) ArticleTitleA rapid and sensitive method for the quantification of microgram quanties of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 248–254 Occurrence Handle10.1016/0003-2697(76)90527-3 Occurrence Handle1:CAS:528:DyaE28XksVehtrY%3D Occurrence Handle942051
R. Beffa H.V. Martin P.E. Pilet (1990) ArticleTitleIn vitro oxidation of indoleacetic acid by soluble auxin-oxidases and peroxidases from maize roots Plant Physiol. 94 485–491 Occurrence Handle1:CAS:528:DyaK3MXjvFamug%3D%3D
J.Y. Berthon M.J. Battraw T. Gaspar N. Boyer (1993) ArticleTitleEarly test using phenolic compounds and peroxidase activity to improve in vitro rooting of Sequoiadendron giganteum (Lindl.). Buchholz Saussurea 27 7
H.G. Bray W.V. Thorpe (1954) Analysis of phenolic compounds of interest in metabolism D. Glick (Eds) Methods of Biochemical Analysis NumberInSeriesVol. 1 Intersci Publ. New York 27–52
T.D. Davis B.E Haissig N. Sankhla (1988) Adventitious root formation in cuttings T.R. Dudley (Eds) Advances in Plant Sciences Series NumberInSeriesVol. II Dioscorides Press PortlandOregon 128–142
G.J. DeKlerk (1996) ArticleTitleMarker of adventitious root formation Agronomie 16 609–616
G.J. DeKlerk W.V.D. Krieken J.C. Jong (1999) ArticleTitleThe formation of adventitious roots: new concepts, new possibilities In Vitro Cell Dev. Biol.-Plant. 35 189–199
A.G. Fett-Neto S.L. Teixeira E.A.M. Dasilva R. Sant Annna (1992) ArticleTitleBiochemical and morphological changes during in vitro rhizogenesis in cuttings of Sequoia sempervirens (D. Don) Endl J. Plant Physiol. 140 720–728 Occurrence Handle1:CAS:528:DyaK3sXjs1Slsw%3D%3D
T. Gaspar C. Kevers J.F. Hausman V. Ripetti (1994) Peroxidase activity and endogenous free auxin during adventitious root formation P.J. Lumsden J.R. Nicholas W.J. Davis (Eds) Physiology, Growth and Development of Plants in Culture Kluwer Acad. Publ. Dordrecht, The Netherlands
T. Gaspar C. Kevers J.F. Hausman (1997) Indissociable chief factors in the inductive phase of adventitious rooting A. Altman M. Waisel (Eds) Biology of Root Formation and Development Plenum Press New York
M.T.K Gunasekare P.K. Evans (2000) ArticleTitleIn vitro rooting of microshoots of tea (Camellia sinensis L.) SriLanka J. Tea Sci. 66 IssueID1/2 5–15
B.E. Haissig (1974) ArticleTitleInfluence of auxin and auxin synergists on adventitious root primordium initiation and development N. Z. J. Sci. 4 299–310
Hartmann H.T., Kester D.E. and Davies F.T. 1993. Plant propagation-principles and practices. 5th ed. Prentice Hall of India Pvt. Ltd, pp. 199–255.
J.F. Hausman (1993) ArticleTitleChanges in peroxidase activity, auxin level and ethylene production during root formation by poplar shoots raised in vitro Plant Growth Regul. 13 263–268 Occurrence Handle10.1007/BF00024847 Occurrence Handle1:CAS:528:DyaK2cXislKhtw%3D%3D
J.F. Hausman D. Evers C. Kevers T. Gaspar (1997) ArticleTitleInternal controls of root induction in poplar shoots raised in vitro Angew. Bot. 71 104–107
Hess C.E. 1962. Characterization of the rooting cofactors extracted from Hedera helix L. and Hibiscus rosa-sinensis L. Proc. 16th Int. Hort. Cong., pp. 382–388.
M. Kar D. Mishra (1976) ArticleTitleCatalaseperoxidase and polyphenol oxidase activities during rice leaf senescence Plant Physiol. 57 315–319 Occurrence Handle1:CAS:528:DyaE28XhtFyms7Y%3D
C. Kevers J.F. Hausman O. Fairre-Rampant D. Evers T. Gaspar (1997) ArticleTitleHormonal control of adventitious rooting: progress and questions J. Appl. Bot. 71 71–79 Occurrence Handle1:CAS:528:DyaK2sXotFShs7o%3D
A.Y. Leung (1980) Encyclopedia of Common Natural Ingredients Used in Food, Drugs, and Cosmetics John Wiley and son New York
J.H. Liu D.M. Reid (1992) ArticleTitleAdventitious rooting in hypocotyls of sunflower (Helianthus annuus) seedlings. IV. The role of changes in endogenous free and conjugated indole-3-acetic acid Physiol. Plant. 86 285–292 Occurrence Handle10.1034/j.1399-3054.1992.860214.x Occurrence Handle1:CAS:528:DyaK3sXhvFeksA%3D%3D
Z.H. Liu I.C. Hsiao Y.W. Pan (1996) ArticleTitleEffect of naphthaleneacetic acid on endogenous indole-3-acetic acidPeroxidase and auxin oxidase in hypocotyls cuttings of soybean during root formation Bot. Bull. Acad. Sin. 37 IssueID4 247–253 Occurrence Handle1:CAS:528:DyaK28XmvFSmsrY%3D
M.C. Mato M.L. Rua E. Ferro (1988) ArticleTitleChanges in levels of peroxidases and phenolics during root formation in Vitis cultured in vitro Physiol. Plant. 72 84–88 Occurrence Handle1:CAS:528:DyaL1cXhs1Crt7Y%3D
C.H. Moncousin T. Gaspar (1983) ArticleTitlePeroxidase as a marker for rooting improvement in Cynara scolymus L. cultivated in vitro Biochem. Physiol. Pflanzen 178 263–271 Occurrence Handle1:CAS:528:DyaL3sXhsFOqsLw%3D
T. Murashige F. Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassay with tobacco tissue cultures Physiol. Plant. 15 473–497 Occurrence Handle1:CAS:528:DyaF3sXksFKm
S. Nag K. Saha M.A. Choudhuri (2001) ArticleTitleRole of auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rooting J. Plant Growth Regul. 20 182–194 Occurrence Handle10.1007/s003440010016 Occurrence Handle1:CAS:528:DC%2BD3MXlvF2hs7w%3D
A.C. Nordstrom J. Eliasson (1991) ArticleTitleLevels of endogenous indole-3-acetic acid and indole-3-acetylaspartic acid during adventitious root formation in pea cuttings Physiol. Plant. 82 599–605 Occurrence Handle10.1034/j.1399-3054.1991.820418.x
Y. Sato M. Sugiyama R.J. Gorecki H. Fukuda A. Komamine (1993) ArticleTitleInterrelationship between lignin deposition and the activities of peroxidase isozymes in differentiating tracheary elements of Zinnia Planta. 189 584–589 Occurrence Handle10.1007/BF00198223 Occurrence Handle1:CAS:528:DyaK3sXitlShtb8%3D
M. Sharma A. Sood P.K. Nagar O.M. Prakash P.S. Ahuja (1999) ArticleTitleDirect rooting and hardening of tea microshoots in the field Plant Cell. Tiss. Org. Cult. 58 IssueID2 111–118 Occurrence Handle10.1023/A:1006374526540
Z. Wiesmann J. Riov E. Epstein (1988) ArticleTitleComparison of movement and metabolism of indole-3-acetic acid and indole-3-butyric acid in mung bean cuttings Physiol. Plant. 74 556–560
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rout, G.R. Effect of Auxins on Adventitious Root Development from Single Node Cuttings of Camellia sinensis (L.) Kuntze and Associated Biochemical Changes. Plant Growth Regul 48, 111–117 (2006). https://doi.org/10.1007/s10725-005-5665-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10725-005-5665-1