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Molecular characterization, expression and methylation status analysis of BMP4 gene in skin tissue of Liaoning cashmere goat during hair follicle cycle

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Abstract

Bone morphogenetic protein 4 (BMP4) is a member of the bone morphogenetic protein family (BMPs). It is involved in the development and cycle of hair follicle, as well as, is thought to be a potential candidate gene for cashmere traits in goats. In the present study, we isolated and characterized a full-length open reading frame (ORF) of BMP4 cDNA from the skin tissue of Liaoning cashmere goat, and investigated the transcriptional pattern and methylation status of BMP4 gene in skin tissue of this breed during different stages of hair follicle cycle. The sequence analysis indicated that the isolated cDNA was 1264-bp in length containing a complete ORF of 1230-bp. It encoded a precursor peptide of 409 amino acids with a signal peptide of 19 amino acids. The structural analysis indicated that goat BMP4 contains typical TGF-β propeptide and TGF-β domains. In skin tissue, BMP4 is generally transcribed in an ascendant pattern from anagen to telogen. The methylation level of 5′ flanking regulatory region of BMP4 gene might be involved in its mRNA expression in skin tissue: a higher BMP4 methylation level in skin coincides with a lower expression of BMP4 mRNA. These results from the present work provided a foundation for further insight into the functional and regulatory characteristics of BMP4 in the development and cycle of hair follicle in Liaoning Cashmere goat.

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Acknowledgments

The work was supported financially by the Foundation of Natural Science Project of Liaoning Province, China (No. 2015020758), Scientific and Technology Project of Liaoning Province, China (No. 2014036), the Foundation of Education Department of Liaoning Province, China (No. L2010507), a Foundation for University Talents of Liaoning Province of China (LJQ2013070), and the Projects of Tianzhushan Person of Outstanding Ability of Shenyang Agricultural University (Shenyang, Liaoning, China).

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Authors and Affiliations

  1. Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

    Wen L. Bai, Yun L. Dang, Jiao J. Wang, Rong H. Yin, Ze Y. Wang, Yu B. Zhu, Yu Y. Cong, Hui L. Xue & Liang Deng

  2. Institute of Animal Husbandry Science of Liaoning Province, 130062, Liaoyang, China

    Dan Guo, Shi Q. Wang & Shu H. Yang

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  1. Wen L. Bai
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  4. Rong H. Yin
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Correspondence to Rong H. Yin.

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Bai, W.L., Dang, Y.L., Wang, J.J. et al. Molecular characterization, expression and methylation status analysis of BMP4 gene in skin tissue of Liaoning cashmere goat during hair follicle cycle. Genetica 144, 457–467 (2016). https://doi.org/10.1007/s10709-016-9914-1

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  • DOI: https://doi.org/10.1007/s10709-016-9914-1

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