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Genetic mapping provides evidence for the role of additive and non-additive QTLs in the response of inter-specific hybrids of Eucalyptus to Puccinia psidii rust infection

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Abstract

Eucalypts are susceptible to a wide range of diseases. One of the most important diseases that affect Eucalyptus plantations worldwide is caused by the rust fungus Puccinia psidii. Here, we provide evidence on the complex genetic control of rust resistance in Eucalyptus inter-specific hybrids, by analyzing a number of full-sib families that display different patterns of segregation for rust resistance. These families are totally unrelated to those previously used in other inheritance studies of rust resistance. By using a full genome scan with 114 genetic markers (microsatellites and expressed sequence tag derived microsatellites) we also corroborated the existence and segregation of a resistance locus, explaining 11.5% of the phenotypic variation, on linkage group 3, corresponding to Ppr1. This find represents an additional validation of this locus in totally unrelated pedigree. We have also detected significant additive × additive digenic interactions with LOD >10.0 on several linkage groups. The additive and epistatic QTLs identified explain between 29.8 and 44.8% of the phenotypic variability for rust resistance. The recognition that both additive and non-additive genetic variation (epistasis) are important contributors to rust resistance in eucalypts reveals the complexity of this host-pathogen interaction and helps explain the success that breeding has achieved by selecting rust-resistant clones, where all the additive and non-additive effects are readily captured. The positioning of epistatic QTLs also provides starting points to look for the underlying genes or genomic regions controlling this phenotype on the upcoming E. grandis genome sequence.

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Acknowledgments

This work was supported by FAPEMIG, with a research grant, and by the Brazilian National Research Council, CNPq, with a MSc fellowship to AAA, a post-doc fellowship to LMSG and a research fellowship to ACA, SHB and DG. We are in debt with Ricardo Martins, Guilherme Christo and Marisângela dos Santos for their assistance during the experiments. We also wish to thank the anonymous reviewers for the detailed revision of the manuscript and useful comments.

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Authors and Affiliations

  1. Embrapa Agroenergy, PqEB, Brasilia, 70770-901, DF, Brazil

    Alexandre Alonso Alves

  2. Graduate Program in Genetics and Breeding, Federal University of Viçosa, Viçosa, MG, 36571-000, Brazil

    Alexandre Alonso Alves, Carla Cristina Gonçalves Rosado, Sérgio Hermínio Brommonschenkel & Acelino Couto Alfenas

  3. Department of Plant Pathology, Federal University of Viçosa, Viçosa, MG, 36571-000, Brazil

    Carla Cristina Gonçalves Rosado, Lúcio Mauro da Silva Guimarães, Sérgio Hermínio Brommonschenkel & Acelino Couto Alfenas

  4. Embrapa Genetic Resources and Biotechnology, Brasilia, DF, 70770-970, Brazil

    Danielle Assis Faria & Dario Grattapaglia

  5. Graduate Program in Genomic Sciences, Catholic University of Brasilia, Brasilia, DF, 70790-160, Brazil

    Dario Grattapaglia

  6. Embrapa Wheat, Passo Fundo, RS, 99001-970, Brazil

    Douglas Lau

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  1. Alexandre Alonso Alves
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Correspondence to Acelino Couto Alfenas.

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Alves, A.A., Rosado, C.C.G., Faria, D.A. et al. Genetic mapping provides evidence for the role of additive and non-additive QTLs in the response of inter-specific hybrids of Eucalyptus to Puccinia psidii rust infection. Euphytica 183, 27–38 (2012). https://doi.org/10.1007/s10681-011-0455-5

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