Abstract
Fiber yield and yield components – including lint index (LI), seed index (SI), lint yield (LY), seed cotton yield (SCY) and number of seeds per boll (NSPB) – were investigated on the farm of Huazhong Agricultural University in a population of 69 F2 individuals and corresponding F2:3 families derived from a cross between high-fiber-yield Gossypium hirsutum CV Handan 208 and a low-fiber-yield Gossypium barbadense CV Pima 90. On the basis of the genetic map constructed previously from the same population by Lin et al. (Plant Breed., 2005), quantitative trait locus (QTL) analysis was performed with the software QTL Cartographer V2.0 using composite interval mapping method (LOD ≥ 3.0). A total of 21 QTLs were identified, which were located in 15 linkage groups. The number of QTLs per trait ranged from one to seven. Of these QTLs detected, one affecting LI explained 24.3% of phenotypic variation (PV), five influencing SI explained 16.15–39.21% of PV, seven controlling LY explained 13.01–28.35% of PV, and two controlling SCY explained 22.76 and 39.97% of PV, respectively. Simultaneously, the detected six QTLs for NSPB were located on five linkage groups, which individually explained 28.01–38.32% of the total phenotypic variation. The results would give breeders further insight into the genetic basis of fiber yield.
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Abdelrhman, S.H. & A.H. Abdalla, 1995. Investigations of character association in some upland cotton (Gossypium hirsutum L.) genotypes. I. Yield and yield components. Univ of Khartoum J Agric Sci 3: 1–12.
Adams, K.L., R. Cronn, R. Percifield & J.F. Wendel, 2003. Genes duplicated by polyploidy show unequal contributions to the transcriptome and organ-specific reciprocal silencing. Proc Natl Acad Sci USA 100: 4649–4654.
Basten, C.J., B.S. Weir & Z.B. Zeng, 1994. Zmap – a QTL Cartographer. In: Smith et al. (Eds.), Proc 5th World Congress on Genetics Applied to Livestock Production, Vol. 2, Computing Strategies and Software, pp. 65–66. University of Guelph, Guelph, Ontario, Canada.
Basten, C.J., B.S. Weir & Z.B. Zeng, 1997. QTL Cartographer: Reference manual and tutorial for QTL mapping. Department Of Statistics, North Carolina State University, Raleigh.
Beavis, W.D., 1998. QTL analyses: Power, precision and accuracy. In: A.H. Paterson (Ed.), Molecular Dissection of Complex Traits, pp. 145–162, CRC Press, Boca Raton.
Charmet, G., 2000. Power and accuracy of QTL detection: Simulation studies of one-QTL models. Agronomie 20: 309–323.
Cheatham, C.L., J.N. Jenkins, J.C. McCarty, Jr., C.E. Watson & J.X. Wu, 2003. Genetic variances and combining ability of crosses of American cultivars, Australian cultivars, and wild cottons. J Cotton Sci 7: 16–22.
Frey, K.J. & T. Homer, 1957. Heritability in standard units. Agron J 49: 59–62.
Jiang, C.X., R.J. Wright, K.M. El-Zik & A.H. Paterson, 1998. Polyploid formation created unique avenues for response to selection in Gossypium (cotton). Proc Natl Acad Sci USA 95: 4419–4424.
Kosambi, D.D., 1944. The estimation of map distances from recombination values. Ann Eugen 12: 172–175.
Lacape, J.M., T.B. Nguyen, S. Thibivilliers, B. Bojinov, B. Courtois, R.G. Cantrell, B. Burr & B. Hau, 2003. A combined RFLP–SSR–AFLP map of tetraploid cotton based on a Gossypium hirsutum × Gossypium barbadense backcross population. Genome 46: 612–626.
Lander, E.S. & D. Botstein, 1989. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121: 185–199.
Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & I. Newburg, 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181.
Li, G. & C.F. Quiros, 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theor Appl Genet 103: 455–461.
Lin, Z.X., D.H. He, X.L. Zhang, Y.C. Nie, X.P. Guo, C.D. Feng & J.McD. Stewart, 2005. Linkage map construction and mapping QTLs for cotton fiber quality using SRAP, SSR and RAPD. Plant Breed 124: 180–187.
Lincoln, S., M. Daly & E.S. Lander, 1992. Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report, 2nd Edition, Whitehead Institute, Cambridge, MA.
Mei, M., N.H. Syed, W. Gao, P.M. Thaxton, C.W. Smith, D.M. Stelly & Z.J. Chen, 2004. Genetic mapping and QTL analysis of fiber-related traits in cotton (Gossypium). Theor Appl Genet 108: 280–291.
Meredith, W.R., 2000. Cotton yield progress – why has it reached a plateau? Better Crops 84: 6–9.
Nguyen, T.B., M. Giband, P. Brottier, A.M. Risterucci & J.M. Lacape, 2004. Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theor Appl Genet 109: 167–175.
Paterson, A.H., Y. Saranga, M. Menz, C.X. Jiang & R.J. Wright, 2003. QTL analysis of genotype × environment interactions affecting cotton fiber quality. Theor Appl Genet 106: 384–396.
Reinisch, A.J., J.M. Dong, C.L. Brubaker, D.M. Stelly, J.F. Wendel & A.H. Paterson, 1994. A detailed RFLP map of cotton Gossypium hirsutum × Gossypium barbadense: Chromosome organization and evolution in a disomic polyploid genome. Genetics 138: 829–847.
Rong, J., C. Abbey, J.E. Bowers, C.L. Brubaker, C. Chang, P.W. Chee, T.A. Delmonte, X. Ding, J.J. Garza, B.S. Marler, C. Park, G.J. Pierce, K.M. Rainey, V.K. Rastogi, S.R. Schulze, N.L. Trolinder, J.F. Wendel, T.A. Wilkins, T.D. Williams-Coplin, R.A. Wing, R.J. Wright, X. Zhao, L. Zhu & A.H. Paterson, 2004. A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics 166: 389–417.
Saranga, Y., M. Menz, C.X. Jiang, R.J. Wright, D. Yakir & A.H. Paterson, 2001. Genomic dissection of genotype × environment interactions conferring adaptation of cotton to arid conditions. Genome Res 11: 1988–1995.
Saranga, Y., C.X. Jiang, R.J. Wright, D. Yakir & A.H. Paterson, 2004. Genetic dissection of cotton physiological responses to arid conditions and their inter-relationships with productivity. Plant Cell Environ 27: 263–277.
SAS Institute Inc., 1999. SAS user's Guide, Release 8.01 Edition. SAS Institute, Cary, NC.
Senchina, D.S., I. Alvarez, R.C. Cronn, B. Liu, J. Rong, R.D. Noyes, A.H. Paterson, R.A. Wing, T.A. Wilkins & J.F. Wendel, 2003. Rate variation among nuclear genes and the age of polyploidy in Gossypium. Mol Biol Evol 20: 633–643.
Shappley, Z.W., 1996a. Construction of RFLP linkage groups and mapping of quantitative trait loci in upland cotton (Gossypium hirsutum L.). Ph.D. Dissertation Mississippi State University (dissertation abstract AAG9711761).
Shappley, Z.W., J.N. Jenkins, C.E. Watson, Jr., A.L. Kahler & W.R. Meredith, Jr., 1996b. Establishment of molecular markers and linkage groups in two F2 populations of upland cotton. Theor Appl Genet 92: 915–919.
Shappley, Z.W., J.N. Jenkins, J. Zhu & J.C. McCarty, Jr., 1998a. Quantitative trait loci associated with agronomic and fiber traits of upland cotton. J Cotton Sci 2: 153–163.
Shappley, Z.W., J.N. Jenkins, W.R. Meredith & J.C. McCarty, Jr., 1998b. An RFLP linkage map of upland cotton (Gossypium hirsutum L.). Theor Appl Genet 97: 756–761.
Stuber, C.W., M.D. Edwards & J.F. Wendel, 1987. Molecular marker-facilitated investigations of quantitative trait loci in maize. II. Factors influencing yield and its component traits. Crop Sci 27: 639–648.
Tang, B., J.N. Jenkins, C.E. Watson, J.C. McCarty, Jr. & R.G. Creech, 1996. Evaluation of genetic variances, heritabilities, and correlations for yield and fiber traits among cotton F2 hybrid population. Euphytica 91: 315–322.
Ulloa, M. & W.R. Meredith, Jr., 2000. Genetic linkage map and QTL analysis of agronomic and fiber quality traits in an intraspecific population. J Cotton Sci 4: 161–170.
Wendel, J.F., 1989. New world cottons contain old world cytoplasm. Proc Natl Acad Sci USA 86: 4132–4136.
Wendel, J.F. & R.C. Cronn, 2003. Polyploidy and the evolutionary history of cotton. Adv Agron 78: 139–186.
Zeng, Z.B., 1993. Theoretical basis of separation of multiple linked gene effects on mapping quantitative trait loci. Proc Natl Acad Sci USA. 90: 10972–10976.
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He, DH., Lin, ZX., Zhang, XL. et al. Mapping QTLs of traits contributing to yield and analysis of genetic effects in tetraploid cotton. Euphytica 144, 141–149 (2005). https://doi.org/10.1007/s10681-005-5297-6
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DOI: https://doi.org/10.1007/s10681-005-5297-6