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Identification of Fusarium species associated with soybean root rot in Sichuan Province, China

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Abstract

Soybean root rot is a worldwide soil-borne fungal disease threatening soybean production, causing large loss in yield and quality of soybean. Fusarium species are well recognized as the important causal agent of Fusarium root rot, which are often distinct with respect to various factors in different soybean-producing regions around the world. Recently, Fusarium root rot has been frequently reported in Sichuan Province of China, where is unique in its climate and diverse cropping patterns, but it is still unclear about the predominant Fusarium species and their pathogenicity on soybean. In this study, diseased soybean roots were collected from three regions of Sichuan Province during 2014–2015. Based on morphological characteristics and phylogenetic analysis of nucleotide sequences of the ribosomal internal transcribed spacer region and the translation elongation factor 1-α gene, 78 isolates of Fusarium were identified as nine distinct species. Pathogenicity tests showed that seven species of Fusarium were able to infect soybean, but differed in pathogenicity. F. oxysporum, F. equiseti and F. graminearum were the most aggressive species to soybean, whereas F. fujikuroi and F. verticillioides were not pathogenic to soybean. There was a strong positive correlation of the pathogenicity of Fusarium species with seedling emergence and fresh root weight. In addition, the diversity of Fusarium species varied among soybean-growing regions. To our knowledge, this report on population and pathogenicity of Fusarium species, in particular, F. graminearum, associated with soybean root rot in Sichuan Province of southwest China, will be helpful to provide effective control strategies for the disease.

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Acknowledgments

This work was supported by the Key Science and Technology Support Project of Sichuan Province, China (Grant number: 2015NZ0040).

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Authors and Affiliations

  1. Institution of Ecological Agriculture, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

    Xiaoli Chang, Hao Dai, Duiping Wang, Huanhuan Zhou, Wanqin He, Yao Fu & Jizhi Yang

  2. Department of Plant Pathology, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

    You Zhou, Guoshu Gong & Jing Shang

  3. Sichuan Engineering Research Center for Crop Strip Intercropping system, Chengdu, 611130, People’s Republic of China

    Faisal Ibrahim, Xiaoling Wu, Taiwen Yong, Chun Song & Wenyu Yang

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  1. Xiaoli Chang
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  2. Hao Dai
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Correspondence to Xiaoli Chang.

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All forms of financial support are acknowledged in the contribution. This work does not involve any human participants or animals. All authors have offered the consent to the submission.

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Xiaoli Chang and Hao Dai contribute equally to this work.

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Table S1

Growth parameters and root rot severity (0–4 scale) of diseased soybean seedling inoculated with various Fusarium species for 7 days in a controlled environment (PDF 104 kb)

Fig. S1

Symptoms on soybean seedlings after 7 days inoculation with representative Fusarium isolates (PDF 326 kb)

Fig. S2

Growth situation of soybean seedlings after 20 days inoculation with representative Fusarium isolates (PDF 192 kb)

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Chang, X., Dai, H., Wang, D. et al. Identification of Fusarium species associated with soybean root rot in Sichuan Province, China. Eur J Plant Pathol 151, 563–577 (2018). https://doi.org/10.1007/s10658-017-1410-7

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