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MSCs transplantation with application of G-CSF reduces apoptosis or increases VEGF in rabbit model of myocardial infarction

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Abstract

The purpose of this study was to test whether mesenchymal stem cells (MSCs) transplantation with application of granulocyte colony-stimulating factor (G-CSF) would have beneficial effects on damaged heart in a rabbit model of myocardial infarction (MI). MI was created by ligation of the left anterior descending coronary artery. After induction of MI, 40 New Zealand white rabbits were randomly divided into 8 groups: (1) MSCs injection at 3 days after MI; (2) G-CSF injection at 3 days after MI; (3) MSCs + G-CSF (20 u/kg/day) injection at 3 days after MI; (4) PBS injection at 3 days after MI; (5) MSCs injection at 7 days after MI; (6) G-CSF injection at 7 days after MI; (7) MSCs + G-CSF (20 u/kg/day) injection 7 days after MI; and (8) PBS injection 7 days after MI. TUNEL analysis showed that the apoptotic cells were distributed in the marginal area of MI. In both 3 and 7 days after MI groups, there were less apoptotic cells in the MSCs and MSCs + G-CSF groups as compared with the PBS group (P < 0.05). However, no decrease in apoptosis was observed in the G-CSF only group (P > 0.05). Immunohistochemistry analysis demonstrated that the expression level of vascular endothelial growth factor was higher in the MSCs, MSCs + G-CSF and G-CSF groups as compared with the PBS group. The present study demonstrated a beneficial effect of MSCs transplantation with application of G-CSF in the treatment of rabbit MI.

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Authors and Affiliations

  1. Department of Radiology, Songjiang Branch Hospital of Shanghai Jiaotong University Affiliated First People’s Hospital, Shanghai, 201600, China

    Jia Yang, Jindong Xia & Yao He

  2. Department of Radiology, Shanghai Jiaotong University Affiliated Third People’s Hospital, No. 280 Mohe Road, Shanghai, 201900, China

    Jiangmin Zhao

  3. Department of Radiology, Shanghai Jiaotong University Affiliated First People’s Hospital, Shanghai, 200080, China

    Guixiang Zhang

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  1. Jia Yang
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  2. Jindong Xia
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  4. Jiangmin Zhao
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  5. Guixiang Zhang
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Correspondence to Jiangmin Zhao or Guixiang Zhang.

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Jia Yang and Jindong Xia are co-first authors.

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Yang, J., Xia, J., He, Y. et al. MSCs transplantation with application of G-CSF reduces apoptosis or increases VEGF in rabbit model of myocardial infarction. Cytotechnology 67, 27–37 (2015). https://doi.org/10.1007/s10616-013-9655-2

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