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Characterization of cell seeding and specific capture of B cells in microbubble well arrays

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Abstract

Development of micro-well array systems for use in high-throughput screening of rare cells requires a detailed understanding of the factors that impact the specific capture of cells in wells and the distribution statistics of the number of cells deposited into wells. In this study we investigate the development of microbubble (MB) well array technology for sorting antigen-specific B-cells. Using Poisson statistics we delineate the important role that the fractional area of MB well opening and the cell seeding density have on determining cell seeding distribution in wells. The unique architecture of the MB well hinders captured cells from escaping the well and provides a unique microenvironmental niche that enables media changes as needed for extended cell culture. Using cell lines and primary B and T cells isolated from human peripheral blood we demonstrate the use of affinity capture agents coated in the MB wells to enrich for the selective capture of B cells. Important differences were noted in the efficacy of bovine serum albumin to block the nonspecific adsorption of primary cells relative to cell lines as well as the efficacy of the capture coatings using mixed primary B and T cells samples. These results emphasize the importance of using primary cells in technology development and suggest the need to utilize B cell capture agents that are insensitive to cell activation.

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Acknowledgements

This work was supported in part by the NSF CBET-0827862, the University of Rochester Developmental Center for AIDS Research grant P30AI078498 (NIH/NIAID), the University of Rochester Autoimmunity Center of Excellence U19AI056390 and by a UL1 TR000038 grant from the National Center for Advancing Translational Sciences, National Institutes of Health. The authors would like to thank Bo Zheng for the preparation of primary lymphocytes. The authors confirm that there are no known conflicts of interest associated with this publication.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Rochester, Rochester, NY, USA

    Meghan C. Jones & Lisa A. DeLouise

  2. Department of Medicine, Infectious Diseases Division, University of Rochester Medical Center, Rochester, NY, USA

    James J. Kobie

  3. Department of Dermatology, University of Rochester Medical Center, Rochester, NY, USA

    Lisa A. DeLouise

  4. 601 Elmwood Ave., Box 697, Rochester, NY, 14642, USA

    Lisa A. DeLouise

Authors
  1. Meghan C. Jones
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  2. James J. Kobie
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  3. Lisa A. DeLouise
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Correspondence to Lisa A. DeLouise.

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Jones, M.C., Kobie, J.J. & DeLouise, L.A. Characterization of cell seeding and specific capture of B cells in microbubble well arrays. Biomed Microdevices 15, 453–463 (2013). https://doi.org/10.1007/s10544-013-9745-0

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