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Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force

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Abstract

When nerve growth factor (NGF) is interacted with PC12 cells derived from rat pheochromocytoma, they are partially differentiated into neuron-like cells with neurites. In this work, PC12 cells differentiated by NGF were selectively isolated using a localized impulsive force in a μm-scale area, which was generated by focusing an infrared femtosecond laser into a cell culture medium. In order to evaluate the ability of the isolation method, differentiated and undifferentiated cells were isolated and their morphological changes after the isolation were compared. In both cases, their neurites were once contracted and some of them gradually regenerated day by day. When differentiated cells were isolated, the percentage of differentiated cells with regenerated neurites, 6 h after the isolation, was about 3.3 times higher than that when undifferentiated ones were isolated. This result was compared with a control trypsin experiment. In the comparison, it was indicated that the same degree of cell function was maintained when the present isolation method was used.

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Acknowledgements

This work was partly supported by CREST from JST (Japan Science and Technology Agency) and by the Grant-in-Aid for Young Scientists (A) from JSPS (Japan Society for the Promotion of Science) of Japan to YH. The support by the Grant-Aid on Priority Area “Bio Manipulation,” from MEXT (Ministry of Education, Culture, Sports, Science and Technology) of Japan and by MOE-ATU project (National Chiao Tung University) from the Ministry of Education, Taiwan, and National Science Consul of Taiwan (0970027441) to HM is also acknowledged.

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Authors and Affiliations

  1. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, Japan

    Yasuyo Maezawa, Kazunori Okano, Mie Matsubara, Hiroshi Masuhara & Yoichiroh Hosokawa

  2. Kansei Fukushi Research Center, Tohoku Fukushi University, 6-149-1, Kunimigaoka, Aoba-ku, Sendai, Japan

    Kazunori Okano

  3. Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, 1001 Ta-Hsueh Road, Hsinchu, 30010, Taiwan

    Hiroshi Masuhara

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  1. Yasuyo Maezawa
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  2. Kazunori Okano
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  3. Mie Matsubara
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  4. Hiroshi Masuhara
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  5. Yoichiroh Hosokawa
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Correspondence to Yoichiroh Hosokawa.

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Maezawa, Y., Okano, K., Matsubara, M. et al. Morphological evaluation of cell differentiation after the isolation of single cells by a femtosecond laser-induced impulsive force. Biomed Microdevices 13, 117–122 (2011). https://doi.org/10.1007/s10544-010-9476-4

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  • DOI: https://doi.org/10.1007/s10544-010-9476-4

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