Abstract
The conversion of β-carotene to retinal by a recombinant β-carotene 15,15′-dioxygenase (Blh protein) from an unculturable marine bacterium was optimized in aqueous solution. Toluene was optimal solvent for the dissolution of β-carotene and the optimal solution for the conversion reaction contained 2.4% (w/v) Tween 20, 0.15 U enzyme/ml, and 350 mg β-carotene/l. Under these conditions, the enzyme produced 181 mg retinal/l after 20 h. This is the highest reported value for the retinal concentration from β-carotene.
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This study was supported by a grant (2009-0084490) from the Basic Research Program, Ministry of Education, Science and Technology.
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Kim, YS., Park, CS. & Oh, DK. Retinal production from β-carotene by β-carotene 15,15′-dioxygenase from an unculturable marine bacterium. Biotechnol Lett 32, 957–961 (2010). https://doi.org/10.1007/s10529-010-0239-3
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DOI: https://doi.org/10.1007/s10529-010-0239-3