Abstract
A novel actinomycete strain, designated as MG28T, was isolated from rhizosphere soil of Akebia trifoliate. The taxonomic position of the strain was investigated by using a polyphasic approach. BLAST search of the full-length 16S rRNA gene sequence of strain MG28T indicated it represented a member of the genus Streptomyces, and displayed 99.03%, 98.90%, 98.90%, 98.89%, 98.83% and less than 98.70% sequence similarities with S. phaeolivaceus GY16T, S. deccanensis KCTC 19241T, S. europaeiscabiei KACC 20186T, S. fructofermentans CGMCC 4.1593T, S. scabiei NRRL B-16523T and other species of the genus Streptomyces with validly published names, respectively. Phylogenomic analysis indicated that strain MG28T was closely related to Streptomyces deccanensis KCTC 19241T. However, the average nucleotide identity values and the digital DNA-DNA hybridization values between them indicated that strain MG28T represented a distinct species. Furthermore, strain MG28T was also distinctly differentiated from strain KCTC 19241T by morphological, physiological and biochemical characteristics. Therefore, strain MG28T (= MCCC 1K06895T = JCM 34922T) represents a novel species of the genus Streptomyces, for which the name Streptomyces akebiae sp. nov. is proposed.
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All data generated or analyzed during this study are included in this published article and its supplementary information files.
Abbreviations
- MCCC:
-
Marine Culture Collection of China
- ISP:
-
International Streptomyces Project
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Acknowledgements
The authors thank Shuang Wan and Wangjie Xu (Wuhan Benagen Technology Co., Ltd, Hubei, China), Yongpeng Yang (Marine Culture Collection of China, MCCC, Xiamen, China) and CICC (China Centre of Industrial Culture Collection, Beijing, China) for providing excellent technical assistance.
Funding
This work was supported by Education Department of Hunan Province (21C0515), Natural Science Foundation of Hunan Province (2021JJ50024), Key projects of Hunan Provincial Department of Education (19A340), the Doctoral Start-up project of Hunan University of Arts and Science (21BSQD09 and 21BSQD10) and Innovation Team of Microbial Technology in Hunan University of Arts and Science.
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WZ and JG conceived the idea of the study; PM, KL, JZ, FZ, YC, XL, XL, KH, WZ and JG analysed the data; WZ and JG interpreted the results. PM, KL, WZ and JG wrote the paper. All authors discussed the results and revised the manuscript.
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The GenBank/EMBL/DDBJ accession numbers for the full-length 16S rRNA gene sequence and genome sequence of strain MG28T and Streptomyces deccanensis KCTC 19241T are OM368590 and OM807210, and CP080647 and CP092431, respectively.
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Mo, P., Li, K., Zhou, J. et al. Streptomyces akebiae sp. nov., a novel actinomycete isolated from rhizosphere soil of Akebia trifoliate. Antonie van Leeuwenhoek 115, 1297–1305 (2022). https://doi.org/10.1007/s10482-022-01772-2
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DOI: https://doi.org/10.1007/s10482-022-01772-2