Abstract
Microbially synthesized nanoparticles has received increasing attentions owing to the broad applications in biology and medicine. In this study, we report a novel bacterium that biologically generates silver nanoparticles (AgNPs). This bacterium, designated strain F202Z8T, was isolated from a rusty iron plate found in the intertidal region of Taean, South Korea. The morphological, biochemical and molecular characteristics predicted that strain F202Z8T belongs to the family Flavobacteriaceae. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain F202Z8T forms a distinct lineage with closely related genera Maribacter, Pelagihabitans, Pseudozobellia, Zobellia, Pricia, and Costertonia and showed the highest similarity to Maribacter aestuarii GY20T (94.5%). The digital DNA–DNA hybridization and average nucleotide identity values calculated from the whole genome-sequence comparison between strain F202Z8T and other members of the family Flavobacteriaceae were in the ranges of 12.7%–16.9% and 70.3%–74.4%, respectively, suggesting that strain F202Z8T represented a novel genus. The complete genome sequence of strain F202Z8T is 4,723,614 bp, with 43.26% G + C content. Based on the COG, GO, KEGG, NR, and Swiss-Prot databases, the genomic analysis of F202Z8T revealed the presence of 17 putative genes responsible for the synthesis of AgNPs. Our polyphasic taxonomic results suggested that this strain represents a novel species of a novel genus in the family Flavobacteriaceae, for which the name Aggregatimonas sangjinii gen. nov., sp. nov. is proposed. The type strain of Aggregatimonas sangjinii is F202Z8T (= KCCM 43411T = LMG 31494T). Overall, our data provide fundamental information to potentially utilize this novel bacterium for synthesis of nanoparticles.
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Acknowledgements
We thank Professor Aharon Oren for his help with etymology. This work was supported by a grant from the National Marine Biodiversity Institute of Korea (2022M00300).
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This work was supported by a grant from the National Marine Biodiversity Institute of Korea (2022M00300).
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DC and YMK performed all experiments, data analysis, and manuscript finalization. JJ performed additional phylogenomic analysis and interpretation. JYHK and KWK participated in the review of the manuscript and isolation of this strain. All authors have read and approved the manuscript.
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Chung, D., Jung, J., Kim, J.Y.H. et al. Aggregatimonas sangjinii gen. nov., sp. nov., a novel silver nanoparticle synthesizing bacterium belonging to the family Flavobacteriaceae. Antonie van Leeuwenhoek 115, 325–335 (2022). https://doi.org/10.1007/s10482-021-01700-w
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DOI: https://doi.org/10.1007/s10482-021-01700-w