Abstract
Chemo-organotrophic iodide (I−)-oxidizing bacterial strains Hi-2T and Mie-1 were isolated from iodide-rich natural gas brine water in Chiba and surface seawater in Mie, Japan, respectively. Cells of strains Hi-2T and Mie-1 were aerobic, Gram-negative and rod-shaped (0.3–0.5 µm width and 1.2–4.4 µm in length). Two isolates grew optimally at 30 °C, pH 7.5 and with 3% NaCl (w/v). Iodide oxidation to form molecular iodine (I2) was a biochemically unique trait for strains Hi-2T and Mie-1. The major cellular fatty acids are C18:1ω7c, C16:1ω5c and C18:1 2-OH. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that strains Hi-2T and Mie-1 were located near Iodidimonas muriae C-3T with 99.2% sequence similarity. The calculated digital DNA–DNA hybridization (dDDH) value of 65.7–65.9% between the two isolates and I. muriae C-3T was lower than the threshold of 70%, which was used for prokaryotic species delineation. Strains Hi-2T and Mie-1 differed in the hydrolysis of aesculin, the hydrolysis of gelatin and the major cellular fatty acids composition from I. muriae C-3T. Considering these biochemical properties, the major cellular fatty acids composition and dDDH value, a novel species is proposed for strains Hi-2T (= JCM 17844T = LMG 28661T) and Mie-1 (= JCM 17845 = LMG 28662), to be named Iodidimonas gelatinilytica.
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Acknowledgements
We thank Prof. Aahron Oren (The Hebrew University of Jerusalem) for helpful corrections of the proposed name and its etymology.
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This work was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science (Grant No. 19H05689).
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TI carried out all morphological, phenotypic and chemotaxonomic characterisation, constructed the 16S rRNA gene phylogenetic trees, did genomic analyses and drafted the original manuscript. KO and MH determined genome sequence of strains C-3T, Hi-2T and Mie-1. MO supervised the research and provided the research funding. SA isolated strains Hi-2T and Mie-1 and carried out the primal characterisation. All authors proofread the manuscript.
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Iino, T., Oshima, K., Hattori, M. et al. Iodidimonas gelatinilytica sp. nov., aerobic iodide-oxidizing bacteria isolated from brine water and surface seawater. Antonie van Leeuwenhoek 114, 625–631 (2021). https://doi.org/10.1007/s10482-021-01546-2
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DOI: https://doi.org/10.1007/s10482-021-01546-2