Abstract
A novel extremely halophilic archaeon, strain N1521T, was isolated from a saline lake in Tibet, China. Cells of the strain were pleomorphic and Gram-stain-negative. It produced red pigments. Growth was observed at 4–42 °C (optimum, 37 °C), pH 7.0–10.5 (optimum, 8.0–9.5), NaCl 11%–25% (optimum, 15%) and in the presence of 0–0.1 M MgCl2 (optimum, 0.05 M) in aerobic conditions. The minimum NaCl concentration that prevented cell lysis was 2% (w/v). The major polar lipids of strain N1521T were phosphatidylglycerol sulfate, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol and an unidentified glycolipid. The DNA G + C content was 58.37 mol%. According to 16S rRNA gene sequence comparisons, strain N1521T revealed the highest sequence similarity to Haloprofundus halophilus NK23T (91.38%) and Halogranum amylolyticum TNN58T (91.00%), and low sequence similarities (< 91%) with other genera in the order Haloferacales. Phylogenetic analysis based on the 16S rRNA gene and rpoB′ gene sequence showed that strain N1521T was distinct from the members of the order Haloferacales. The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values calculated from whole genome-sequence comparison between strain N1521T and the members of the order Haloferacales were in the ranges of 15.1–18.2%, 68.8–73.0%, and 58.4–63.9%, respectively. Phylogenetic tree reconstructions based on the whole-genome sequences revealed that strain N1521T was closer to the members of the family Halorubraceae. Based on the data obtained, strain N1521T is thus considered to represent a novel species of a new genus within the family Halorubraceae, for which the name Halalkalirubrum salinum gen. nov., sp. nov. is proposed. The type strain is N1521T (= CGMCC 1.16693 = JCM 33785).
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Funding
This work was funded by the National Natural Science Foundation of China (Grant No. 91751201), the National Science and Technology Foundation Project of China (Grant No. 2015FY110100), the Science and Technology Basic Resources Investigation Project (Grant No. 2017FY100300) and the National Natural Science Foundation of China (Grant No. 31970031)
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Strains N1521T was isolated by ZZ. Data collection and analysis were performed by ZZ, JZ, DZ and JH. ZZ and HX conceived the project and prepared the manuscript. All authors read and approved the final manuscript.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and rpoB′ gene sequences of strain N1521T are MK228974 and MK241978, respectively. The genome accession number of strain N1521T is SRSG01000000.
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Zuo, Z., Zhao, D., Zhou, J. et al. Halalkalirubrum salinum gen. nov., sp. nov., a halophilic archaeon isolated from a saline lake. Antonie van Leeuwenhoek 114, 83–94 (2021). https://doi.org/10.1007/s10482-020-01502-6
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DOI: https://doi.org/10.1007/s10482-020-01502-6