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Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment

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Abstract

A Gram-stain negative, strictly aerobic, rod-shaped, non-motile bacterium, designated strain DY46T, was isolated from Atlantic Ocean sediment. The isolate was found to grow in medium containing 0–3.0 % (w/v) NaCl (optimally at 0–1.0 %), at 4–37 °C and pH 5.0–8.0. Chemotaxonomic analysis detected MK-6 as the sole isoprenoid quinone. The major fatty acids were identified iso-C15:0, iso-C17:0 3-OH, iso-C17:1 ω9c and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 ω7c). The DNA G + C content was determined to be 40.7 mol %. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain DY46T falls within the cluster comprising Chryseobacterium species. The levels of 16S rRNA gene sequence similarity between strain DY46T and the type strains of the Chryseobacterium species with validly published names ranged from 92.4 to 99.1 %, the high values (>97 %) being with Chryseobacterium takakiae A1-2T (99.1 %), C. taiwanense BCRC 17412T (98.0 %), C. taeanense PHA3-4T (97.3 %), C. hispalense DSM 25574T (97.3 %), C. camelliae THG C4-1T (97.2 %), C. gregarium DSM 19109T (97.1 %) and C. wanjuense R2A10-2T (97.0 %). The DNA–DNA relatedness values between strain DY46T and the type strains of the above closely related species were 47, 57, 24, 34, 6, 40 and 21 %, respectively. On the basis of phenotypic and genotypic characteristics, strain DY46T represents a novel member within the genus Chryseobacterium, for which the name Chryseobacterium profundimaris is proposed. The type strain is DY46T (=CGMCC 1.12663T = JCM 19801T).

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Acknowledgments

This work was supported by grants from China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-R-03 and DY125-14-E-02) and the National Natural Science Foundation of China (41276173).

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Authors and Affiliations

  1. Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, 36th North BaoChu Road, Hangzhou, 310012, People’s Republic of China

    Lin Xu, Ying-Yi Huo, Zheng-Yang Li, Chun-Sheng Wang & Xue-Wei Xu

  2. Department of Plant and Environmental Sciences, the Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, The Edmond J. Safra Campus, Givat Ram, 91904, Jerusalem, Israel

    Aharon Oren

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  1. Lin Xu
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Correspondence to Xue-Wei Xu.

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Xu, L., Huo, YY., Li, ZY. et al. Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment. Antonie van Leeuwenhoek 107, 979–989 (2015). https://doi.org/10.1007/s10482-015-0390-x

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